A1KAK4 (ARGB_AZOSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylglutamate kinase
EC=2.7.2.8

Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferase
NAG kinase
Short name=AGK

Gene names

Name:	argB
Ordered Locus Names:	azo3244

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082_B
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00082_B.
Sequence similarities	Belongs to the acetylglutamate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetylglutamate kinase activity Inferred from electronic annotation. Source: EC glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Acetylglutamate kinase HAMAP MF_00082_B		PRO_1000010482
Regions
	Region	72 – 73	2	Substrate binding By similarity
Sites
	Binding site	94	1	Substrate By similarity
	Binding site	197	1	Substrate By similarity
	Site	37	1	Transition state stabilizer By similarity
	Site	257	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1KAK4 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: E55BC4BD6F930FE4	FASTA	300	31,522
	10 20 30 40 50 60 MSAASSTESV TPALKAEILA EALPYIKRFF DKTIVIKYGG NAMTDPHLKD CFARDVVLLK 70 80 90 100 110 120 LVGLNPVVVH GGGPQIETLL AKVGKKGEFV QGMRVTDAET MEVVEMVLGG QVNKEIVNLI 130 140 150 160 170 180 NQAGGKAVGL TGKDASFIRA KKLLMQKLDA PAGDVIDVGQ VGEITTIDPS LISFLDQGDF 190 200 210 220 230 240 IPVIAPIGVG DNGETYNINA DVVAGKLAEI LKAEKLVLLT NTPGVLDKAG NLLTGLTPRQ 250 260 270 280 290 300 IDDLVADGTL SGGMLPKIGS ALDAARNGVK SVHIIDGRVE HCLLLEILTD HGVGTMIKSK

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BH72.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM406670 Genomic DNA. Translation: CAL95860.1.
RefSeq	YP_934746.1. NC_008702.1.
3D structure databases
ProteinModelPortal	A1KAK4.
SMR	A1KAK4. Positions 16-300.
ModBase	Search...
Protein-protein interaction databases
STRING	A1KAK4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4609038.
GenomeReviews	Gene locus azo3244 in contig AM406670_GR.
KEGG	azo:azo3244.
PATRIC	21014942. VBIAzoSp26047_3293.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0548.
HOGENOM	HBG497643.
OMA	GGNAMID.
PhylomeDB	A1KAK4.
ProtClustDB	PRK00942.
Enzyme and pathway databases
BioCyc	ASP62928:AZO3244-MONOMER.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00930.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00761. ArgB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_AZOSB

Accession

Primary (citable) accession number: A1KAK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program