A1KAJ9 (SYD_AZOSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate--tRNA ligase
EC=6.1.1.12

Alternative name(s):
Aspartyl-tRNA synthetase
Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	azo3239

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	599 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044_B
Subunit structure	Homodimer By similarity. HAMAP MF_00044_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00044_B.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW aspartate-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 599	599	Aspartate--tRNA ligase HAMAP MF_00044_B		PRO_1000006634

Sequences

	Sequence		Length	Mass (Da)
	A1KAJ9 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 8E75C1BCABE80703	FASTA	599	67,094
	10 20 30 40 50 60 MRTQYCGQVT AAHLDQIVTL CGWVHRRRDH GGVIFIDLRD REGLVQVVCD PDRAEMFKTA 70 80 90 100 110 120 ESVRNEFVLK MTGKVRRRPA GTENANLTSG EIEVLCHEIE VLNTSATPPF QLDDENLSET 130 140 150 160 170 180 VRLTNRVIDL RRPQMQKNLM LRYKVAMAFR RFLDGAGFID VETPMLTKST PEGARDYLVP 190 200 210 220 230 240 SRVHPGQFFA LPQSPQLFKQ LLMVAGFDRY YQITKCFRDE DLRADRQPEF TQVDIETSFL 250 260 270 280 290 300 NETEITALME EMIRFVFKES LGVDLPSPFP RMTYAEAMRR YGSDKPDLRV TLELTEVTDL 310 320 330 340 350 360 MKDVAFKVFS GPANSGGRVA AMRVPGGATL TRGEIDEYTK FVGIYGAKGL AYIKVNDASQ 370 380 390 400 410 420 PNETGLQSPI VKNLSEAALR GVIERTGAQS GDLIFFGADK AKVVNDAIGA LRIKLGHEKG 430 440 450 460 470 480 YVTGDAWRPL WVVDFPMFEY DEDDKRWTAC HHPFTSPKDE HVELLKTNPG ECLAKAYDLA 490 500 510 520 530 540 LNGWEIGGGS VRIHRADVQA TVFEALNIGA EEQQVKFGFL LDALKFGAPP HGGLAFGLDR 550 560 570 580 590 VVTMMAGAES IRDVIAFPKT QRAQCLLTNA PGEVDEKQLR ELHIRLRQKV ETQVEVGKA

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BH72.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM406670 Genomic DNA. Translation: CAL95855.1.
RefSeq	YP_934741.1. NC_008702.1.
3D structure databases
ProteinModelPortal	A1KAJ9.
SMR	A1KAJ9. Positions 1-587.
ModBase	Search...
Protein-protein interaction databases
STRING	A1KAJ9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4607862.
GenomeReviews	Gene locus azo3239 in contig AM406670_GR.
KEGG	azo:azo3239.
PATRIC	21014932. VBIAzoSp26047_3288.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0173.
HOGENOM	HBG396032.
OMA	AFPKTQQ.
PhylomeDB	A1KAJ9.
ProtClustDB	PRK00476.
Enzyme and pathway databases
BioCyc	ASP62928:AZO3239-MONOMER.
Family and domain databases
HAMAP	MF_00044_B. Asp_tRNA_synth_B. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR004115. GAD_dom. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:3.30.1360.30. GAD_dom. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01876.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF55261. SSF55261. 1 hit.
TIGRFAMs	TIGR00459. AspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_AZOSB

Accession

Primary (citable) accession number: A1KAJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program