A1KAI4 (GCP_AZOSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp

Alternative name(s):
t(6)A37 threonylcarbamoyladenosine biosynthesis protein

Gene names

Name:	gcp
Ordered Locus Names:	azo3224

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine By similarity. HAMAP MF_01445
Subcellular location	Cytoplasm Potential HAMAP MF_01445.
Sequence similarities	Belongs to the KAE1 / YgjD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Probable tRNA threonylcarbamoyladenosine biosynthesis protein Gcp HAMAP MF_01445		PRO_0000303260

Sequences

	Sequence		Length	Mass (Da)
	A1KAI4 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 417F277A48CAF1B4	FASTA	343	36,173
	10 20 30 40 50 60 MKVLGIETSC DETGVAIYDG SVRRLLGHCL HSQIDLHAAY GGVVPELASR DHIRRLPLLL 70 80 90 100 110 120 KQLFGETGLS RRDIDAVAYT AGPGLAGALL TGASFAEAFA LAAGVPALPI HHLEGHLLSP 130 140 150 160 170 180 LLADDAPRFP FVALLVSGGH TQLMHVAGVG RYELLGESLD DAAGEAFDKT AKLLGLGYPG 190 200 210 220 230 240 GPQLSALAAQ GCAGRFKLPR PMLHSGDFDF SFSGLKTAVL NRVSSDDWEP GLAADLAADF 250 260 270 280 290 300 QEAVVEVLCA KALAALKHTG LDTLVVAGGV GANRRLRERL DNLAARRRYR VCYPEPALCT 310 320 330 340 DNGAMIAFAG ALRLEAGERR AAAPAVRVRP RWPLVELDAP GCP

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BH72.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM406670 Genomic DNA. Translation: CAL95840.1.
RefSeq	YP_934726.1. NC_008702.1.
3D structure databases
ProteinModelPortal	A1KAI4.
ModBase	Search...
Protein-protein interaction databases
STRING	A1KAI4.
Protein family/group databases
MEROPS	M22.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4607856.
GenomeReviews	Gene locus azo3224 in contig AM406670_GR.
KEGG	azo:azo3224.
PATRIC	21014902. VBIAzoSp26047_3273.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0533.
HOGENOM	HBG304663.
OMA	PAVGVHH.
PhylomeDB	A1KAI4.
ProtClustDB	PRK09604.
Enzyme and pathway databases
BioCyc	ASP62928:AZO3224-MONOMER.
Family and domain databases
HAMAP	MF_01445. gcp. [Tree]
InterPro	IPR022450. Pept_M22_O-sialoglycopept. IPR000905. Peptidase_M22. IPR017861. Peptidase_M22_subgr. [Graphical view]
KO	K01409.
Pfam	PF00814. Peptidase_M22. 1 hit. [Graphical view]
PRINTS	PR00789. OSIALOPTASE.
TIGRFAMs	TIGR03723. Bact_gcp. 1 hit. TIGR00329. Gcp_kae1. 1 hit.
PROSITE	PS01016. GLYCOPROTEASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCP_AZOSB

Accession

Primary (citable) accession number: A1KAI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program