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A1KAH5 (SYL_AZOSB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:azo3215
OrganismAzoarcus sp. (strain BH72) [Complete proteome] [HAMAP]
Taxonomic identifier62928 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAzoarcus

Protein attributes

Sequence length873 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 873873Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009290

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif628 – 6325"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6311ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A1KAH5 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 9516D7967993F4CB

FASTA87396,943
        10         20         30         40         50         60 
MQDKYQPAAV ETAAQQHWES TAAFRVTEDA TRPKYYCLSM FPYPSGKLHM GHVRNYTIGD 

        70         80         90        100        110        120 
VLARFHRMRG YNVLQPMGWD AFGMPAENAA IQNNVPPAKW TYANIDYMKG QLKRLGFAID 

       130        140        150        160        170        180 
WSREVATCTP EYYRWEQWLF TRLFEKGLIY KKLGTVNWDP VDETVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
ALVEKREIPM YYMKITAYAD ELLEALDGLP GWPEQVKLMQ KNWIGRSEGV EVHFPYDLST 

       250        260        270        280        290        300 
IGSSGVLKVF TTRGDTLMGA TYVAVAAEHP LATQAAAGNP ELAAFIDECK QGGVAEADLA 

       310        320        330        340        350        360 
TMEKKGMPTG LRVVHPITGE YLPVWVANYV LMGYGEGAVM AVPAHDERDF AFATKYKLPI 

       370        380        390        400        410        420 
KMVVRSTHDA YENVAAPWID AYAEHGKLVN SGKYDNLHFQ DAVDAIAADL AAKGLGNKRV 

       430        440        450        460        470        480 
QYRLRDWGIS RQRYWGCPIP MVRCDDCGDV PVPDEQLPVV LPENVEITGR GSPLAKMPEF 

       490        500        510        520        530        540 
YQCSCPKCGK PARRETDTMD TFVESSWYFL RYASPDSTTA MVDERVNYWA PVDQYIGGIE 

       550        560        570        580        590        600 
HAILHLLYSR FFTRAMRDCG LVNVSEPFTN LLTQGMVVAE TYYRELDGGK KQWLNPADVQ 

       610        620        630        640        650        660 
VERDERGRIT AAKLASDGLP VVIGGTEKMS KSKNNGVDPQ ALVDQYGADT ARLFIIFAAP 

       670        680        690        700        710        720 
PDQQLEWSDS GVEGASRFLR RVWNFGHAFA SEFRAALPAA RALAAGTLPA ALADVRREIH 

       730        740        750        760        770        780 
THLKQANYDF GKHQFNTVVS AAMKILNALE KAPRDDAAAH AEVAEEGFSI LVRLLSPITP 

       790        800        810        820        830        840 
HIAHALWQDC GFGGDIVQAA WPEPLEAALK QDEIELMLQV NGKLRGSVKV AADAGKSDIE 

       850        860        870 
ALALASEAAQ KFMEGKPPKK VVVVPGRLVN IVV 

« Hide

References

[1]"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72."
Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. expand/collapse author list , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A.
Nat. Biotechnol. 24:1385-1391(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BH72.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM406670 Genomic DNA. Translation: CAL95831.1.
RefSeqYP_934717.1. NC_008702.1.

3D structure databases

ProteinModelPortalA1KAH5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING62928.azo3215.

Proteomic databases

PRIDEA1KAH5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL95831; CAL95831; azo3215.
GeneID4607854.
KEGGazo:azo3215.
PATRIC21014884. VBIAzoSp26047_3264.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAVVHCDAC.
OrthoDBEOG63Z74X.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 2 hits.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_AZOSB
AccessionPrimary (citable) accession number: A1KAH5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: May 14, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries