Reviewed, UniProtKB/Swiss-Prot A1KAG3 (SYM_AZOSB)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Ordered Locus Names:	azo3203

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	723 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily. Contains 1 tRNA-binding domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 723	723	Methionyl-tRNA synthetase HAMAP MF_00098		PRO_0000331779
Regions
	Domain	612 – 723	112	tRNA-binding
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00098
	Motif	345 – 349	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	143	1	Zinc By similarity
	Metal binding	146	1	Zinc By similarity
	Metal binding	156	1	Zinc By similarity
	Metal binding	159	1	Zinc By similarity
	Binding site	348	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1KAG3-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 1FDA65CAC43E41E7	FASTA	723	80,120
	10 20 30 40 50 60 MSRKILVTNA LPYANGDIHL GHLVGYIQGD IWVRFQRMRG NSVHYVCADD THGTPIMLRA 70 80 90 100 110 120 EKEGITPEQL IDRVHGEHLR DFTDFGVGFD HYHSTHSAEN RAYAEEIYGK LLGAGFIETR 130 140 150 160 170 180 SIEQFYDPVK EMFLPDRFIK GECPKCGAGD QYGDNCEVCG AAYAPTELKN PYSAVSGAKP 190 200 210 220 230 240 VLRTSEHYFF RLSDPRAVEF LRDWTRGTNA GGERRLQAEA ANKMKEWLGE AGENKLSDWD 250 260 270 280 290 300 ISRDAPYFGF EIPGAPGKYF YVWLDAPIGY LASFRHYAER RGDIAVADYT DAARAESHGT 310 320 330 340 350 360 ELVHFIGKDI LYFHALFWPA MLKFAGYRTP TQLCVNGFLT VDGAKMSKSR GTFITARSYI 370 380 390 400 410 420 VQGLNPEWLR YYFASKSNGT MEDVDLNLDD MIAKVNSDLV GKYVNIASRC AGFIAKRFDG 430 440 450 460 470 480 KLGASDPKAT ADFESAYESA QIAQAYDERD YGRALREIMR LADLANQYVN DHKPWELAKQ 490 500 510 520 530 540 DGQDAQLHVV CSTALTLFRD LTLYLKPVLP GLAAKVEAFL ALPPLQWNDS WKPLPAGHAI 550 560 570 580 590 600 TAYQHLMTRV ERKQIDALLE ANRESLAPAA ATAPAKDAKP AKEAGSQQRH AEKQQHAAGV 610 620 630 640 650 660 SETASPHISI DDFTKVDLRI AKIVDAQHVE GADKLIRLQL DIGETDEAGN PKPRQVFAGI 670 680 690 700 710 720 KSAYDPATLV GRLTVMVANL APRKMKFGMS EGMVLAASDP DGKTGGLYIL APDEGAQAGM RVK

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM406670 Genomic DNA. Translation: CAL95819.1.
RefSeq	YP_934705.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1KAG3.
Genome annotation databases
GeneID	4607848.
GenomeReviews	Gene locus azo3203 in contig AM406670_GR.
KEGG	azo:azo3203.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	CEAVPES.
Family and domain databases
HAMAP	MF_00098. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR004495. Met-tRNA-synth_Ia_bsu_C. IPR012340. NA-bd_OB-fold. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. IPR002547. tRNA_bd. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. PF01588. tRNA_bind. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit. TIGR00399. metG_C_term. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. PS50886. TRBD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_AZOSB

Accession

Primary (citable) accession number: A1KAG3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)