Reviewed, UniProtKB/Swiss-Prot A1KAD1 (PROB_AZOSB)

Names and origin

Protein names

Recommended name:
    Glutamate 5-kinase
    EC=2.7.2.11
Alternative name(s):
    Gamma-glutamyl kinase
      Short name=GK

Gene names

Name:	proB
Ordered Locus Names:	azo3170

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5-oxoproline By similarity.
Catalytic activity	ATP + L-glutamate = ADP + L-glutamate 5-phosphate. HAMAP MF_00456
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 1/2. HAMAP MF_00456
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the glutamate 5-kinase family. Contains 1 PUA domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA binding Inferred from electronic annotation. Source: InterPro glutamate 5-kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 371	371	Glutamate 5-kinase HAMAP MF_00456		PRO_1000081035
Regions
	Domain	280 – 357	78	PUA

Sequences

	Sequence		Length	Mass (Da)
	A1KAD1-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 0F3CD9C71E1B26BF	FASTA	371	39,996
	10 20 30 40 50 60 MRDKIKNARR LIVKVGSALV TNNGAGLDPA ALDDWARQIA ALRARGREIV LVSSGAIAAG 70 80 90 100 110 120 MQRLGWVKRP HEMHRLQAAA AVGQMGLVEA YEKAFSRHGL QTAQILLTHE DLADRTRYLN 130 140 150 160 170 180 ARSTLVTLLE LGVVPIINEN DTVVTDEIKF GDNDTLGALV ANLVEADTLI ILTDQRGLYT 190 200 210 220 230 240 ADPRRDPGAT LISEGRAEDR QYEAMAGGAG SGISKGGMIT KIRAAQRAAR SGAHTCIASG 250 260 270 280 290 300 RETDPLLRLA DGEAVGTLLY ADSTPLQARK QWLADHLQLA GSLIVDAGAA RALDDGRSLL 310 320 330 340 350 360 PVGVVEVQGE FKRGAAVACR DEHGTELARG LVNYSSAECR RILRRPTSDI EQLLGYIDEP 370 ELIHRDNLVI R

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM406670 Genomic DNA. Translation: CAL95787.1.
RefSeq	YP_934673.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1KAD1.
Genome annotation databases
GeneID	4607827.
GenomeReviews	Gene locus azo3170 in contig AM406670_GR.
KEGG	azo:azo3170.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	ILTDQRG.
Family and domain databases
HAMAP	MF_00456. [Tree]
InterPro	IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu_5kinase. IPR011529. Glu_5kinase_bact. IPR019797. Glutamate_5-kinase_CS. IPR005715. ProB. IPR002478. PUA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01472. PUA. 1 hit. [Graphical view]
PIRSF	PIRSF000729. GK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SMART	SM00359. PUA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01027. proB. 1 hit.
PROSITE	PS00902. GLUTAMATE_5_KINASE. 1 hit. PS50890. PUA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROB_AZOSB

Accession

Primary (citable) accession number: A1KAD1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)