Reviewed, UniProtKB/Swiss-Prot A1KAB7 (ILVC_AZOSB)

Names and origin

Protein names

Recommended name:
    Ketol-acid reductoisomerase
    EC=1.1.1.86
Alternative name(s):
    Acetohydroxy-acid isomeroreductase
    Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	azo3156

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_1000050478
Sites
	Active site	107	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	A1KAB7-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: D72BF43BDC7C4FB4	FASTA	338	36,909
	10 20 30 40 50 60 MKVYYDKDAD LSLIKGKKVT IVGYGSQGHA HAQNLNDSGV KVTVAVRKGG ASWDKAKAAG 70 80 90 100 110 120 LKVEEIAKAV KTADLVMILL PDENIPQVYK EEVEPNIKKG ATLAFAHGFN VHYNQVVPRE 130 140 150 160 170 180 DLDVIMVAPK GPGHTVRSEY LRGGGVPSLI AVYQDKSGKA KDIALSYAAA NGGTKGGVIE 190 200 210 220 230 240 TNFKEETETD LFGEQAVLCG GAVELVKMGF ETLTEAGYAP EMAYFECLHE LKLIVDLMYE 250 260 270 280 290 300 GGIANMNYSI SNNAEYGEYV TGPEVINAQS REAMRNALKR IQTGEYAKMF IQEGKTNYPS 310 320 330 MTARRRLNAE HPIEQVGGQL RDMMPWIKAK ALVDKSKN

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM406670 Genomic DNA. Translation: CAL95773.1.
RefSeq	YP_934659.1.
3D structure databases
SMR	A1KAB7. Positions 1-327.
ModBase	Search...
Genome annotation databases
GeneID	4609074.
GenomeReviews	Gene locus azo3156 in contig AM406670_GR.
KEGG	azo:azo3156.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A1KAB7. AHGFNIR.
Family and domain databases
HAMAP	MF_00435. [Tree]
InterPro	IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013116. IlvN. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
TIGRFAMs	TIGR00465. ilvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_AZOSB

Accession

Primary (citable) accession number: A1KAB7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)