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Protein

3-methyl-2-oxobutanoate hydroxymethyltransferase

Gene

panB

Organism
Azoarcus sp. (strain BH72)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: (R)-pantothenate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB)
  2. 2-dehydropantoate 2-reductase (apbA1), 2-dehydropantoate 2-reductase (panE1)
This subpathway is part of the pathway (R)-pantothenate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate, the pathway (R)-pantothenate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi51MagnesiumUniRule annotation1
Metal bindingi90MagnesiumUniRule annotation1
Binding sitei90Alpha-ketoisovalerateUniRule annotation1
Binding sitei119Alpha-ketoisovalerateUniRule annotation1
Metal bindingi121MagnesiumUniRule annotation1
Active sitei188Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processPantothenate biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00028; UER00003.

Names & Taxonomyi

Protein namesi
Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferaseUniRule annotation (EC:2.1.2.11UniRule annotation)
Alternative name(s):
Ketopantoate hydroxymethyltransferaseUniRule annotation
Short name:
KPHMTUniRule annotation
Gene namesi
Name:panBUniRule annotation
Ordered Locus Names:azo3144
OrganismiAzoarcus sp. (strain BH72)
Taxonomic identifieri62928 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesZoogloeaceaeAzoarcus
Proteomesi
  • UP000002588 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002972181 – 2713-methyl-2-oxobutanoate hydroxymethyltransferaseAdd BLAST271

Interactioni

Subunit structurei

Homodecamer; pentamer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi62928.azo3144.

Structurei

3D structure databases

ProteinModelPortaliA1KAA5.
SMRiA1KAA5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni51 – 52Alpha-ketoisovalerate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the PanB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCG. Bacteria.
COG0413. LUCA.
HOGENOMiHOG000078427.
KOiK00606.
OMAiGHIGLMP.
OrthoDBiPOG091H02K8.

Family and domain databases

CDDicd06557. KPHMT-like. 1 hit.
HAMAPiMF_00156. PanB. 1 hit.
InterProiView protein in InterPro
IPR003700. Pantoate_hydroxy_MeTrfase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
PANTHERiPTHR20881. PTHR20881. 1 hit.
PfamiView protein in Pfam
PF02548. Pantoate_transf. 1 hit.
PIRSFiPIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR00222. panB. 1 hit.

Sequencei

Sequence statusi: Complete.

A1KAA5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYLQDDKPV TLFELGKMRA EGRKIAMLTC YDASFAALLE RNGVDVLLVG
60 70 80 90 100
DSLGNVLQGQ KSTLPVTLEQ MAYHTECVVR GSSRPFVVTD MPFGSYQESP
110 120 130 140 150
EQAMRNAARL MAAGAEMVKL EGGAFMAETV RFLVERGVPV CAHIGLTPQS
160 170 180 190 200
VHQLGGYRVQ GRSEEGAARL KADALALEQA GAALVVLEMV PAAVASDITR
210 220 230 240 250
SLSTMATIGI GAGADCSGQV LVLHDMIGVY PGKKARFVKN FMEGAASIDA
260 270
AVAAYVAAVK DGSFPAAEHC Y
Length:271
Mass (Da):28,665
Last modified:February 6, 2007 - v1
Checksum:i3512F64C9877D563
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM406670 Genomic DNA. Translation: CAL95761.1.
RefSeqiWP_011766869.1. NC_008702.1.

Genome annotation databases

EnsemblBacteriaiCAL95761; CAL95761; azo3144.
KEGGiazo:azo3144.

Similar proteinsi

Entry informationi

Entry nameiPANB_AZOSB
AccessioniPrimary (citable) accession number: A1KAA5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: February 6, 2007
Last modified: August 30, 2017
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families