A1KAA5 (PANB_AZOSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferase
EC=2.1.2.11

Alternative name(s):
Ketopantoate hydroxymethyltransferase
Short name=KPHMT

Gene names

Name:	panB
Ordered Locus Names:	azo3144

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	271 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity. HAMAP MF_00156
Catalytic activity	5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP MF_00156
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00156
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP MF_00156
Subunit structure	Homodecamer; pentamer of dimers By similarity. HAMAP MF_00156
Subcellular location	Cytoplasm Potential HAMAP MF_00156.
Sequence similarities	Belongs to the PanB family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-methyl-2-oxobutanoate hydroxymethyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 271	271	3-methyl-2-oxobutanoate hydroxymethyltransferase HAMAP MF_00156		PRO_0000297218
Regions
	Region	51 – 52	2	Alpha-ketoisovalerate binding By similarity
Sites
	Active site	188	1	Proton acceptor By similarity
	Metal binding	51	1	Magnesium By similarity
	Metal binding	90	1	Magnesium By similarity
	Metal binding	121	1	Magnesium By similarity
	Binding site	90	1	Alpha-ketoisovalerate By similarity
	Binding site	119	1	Alpha-ketoisovalerate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1KAA5 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 3512F64C9877D563	FASTA	271	28,665
	10 20 30 40 50 60 MSYLQDDKPV TLFELGKMRA EGRKIAMLTC YDASFAALLE RNGVDVLLVG DSLGNVLQGQ 70 80 90 100 110 120 KSTLPVTLEQ MAYHTECVVR GSSRPFVVTD MPFGSYQESP EQAMRNAARL MAAGAEMVKL 130 140 150 160 170 180 EGGAFMAETV RFLVERGVPV CAHIGLTPQS VHQLGGYRVQ GRSEEGAARL KADALALEQA 190 200 210 220 230 240 GAALVVLEMV PAAVASDITR SLSTMATIGI GAGADCSGQV LVLHDMIGVY PGKKARFVKN 250 260 270 FMEGAASIDA AVAAYVAAVK DGSFPAAEHC Y

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BH72.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM406670 Genomic DNA. Translation: CAL95761.1.
RefSeq	YP_934647.1. NC_008702.1.
3D structure databases
ProteinModelPortal	A1KAA5.
SMR	A1KAA5. Positions 10-271.
ModBase	Search...
Protein-protein interaction databases
STRING	A1KAA5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4607817.
GenomeReviews	Gene locus azo3144 in contig AM406670_GR.
KEGG	azo:azo3144.
PATRIC	21014724. VBIAzoSp26047_3190.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0413.
HOGENOM	HBG299908.
OMA	YDATFAH.
PhylomeDB	A1KAA5.
ProtClustDB	PRK00311.
Enzyme and pathway databases
BioCyc	ASP62928:AZO3144-MONOMER.
Family and domain databases
HAMAP	MF_00156. PanB. [Tree]
InterPro	IPR003700. Pantoate_hydroxy_MeTrfase. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
KO	K00606.
PANTHER	PTHR20881. Pantoate_transf. 1 hit.
Pfam	PF02548. Pantoate_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR00222. PanB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANB_AZOSB

Accession

Primary (citable) accession number: A1KAA5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program