Reviewed, UniProtKB/Swiss-Prot A1KA97 (PUR5_AZOSB)

Names and origin

Protein names

Recommended name:
    Phosphoribosylformylglycinamidine cyclo-ligase
    EC=6.3.3.1
Alternative name(s):
    AIRS
    Phosphoribosyl-aminoimidazole synthetase
    AIR synthase

Gene names

Name:	purM
Ordered Locus Names:	azo3136

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole. HAMAP MF_00741
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2. HAMAP MF_00741
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Phosphoribosylformylglycinamidine cyclo-ligase HAMAP MF_00741		PRO_1000192991

Sequences

	Sequence		Length	Mass (Da)
	A1KA97-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 6C990EC755CBBAE7	FASTA	352	37,206
	10 20 30 40 50 60 MSVSNSASPS SLSYRDAGVD IEAGDALVDR IKPFAKRTMR PEVLGGIGGF GALFELSKKF 70 80 90 100 110 120 KEPVLVSGTD GVGTKLKLAF QLNKHDTVGQ DLVAMSVNDI LVQGAEPLFF LDYFACGKLD 130 140 150 160 170 180 VDTAADVVKG IAHGCELAGC ALIGGETAEM PSMYPEGEYD LAGFAVGAVE KADIIDGSKI 190 200 210 220 230 240 VPGDVVLGLA SSGAHSNGYS LVRKIIEVAK PDLDADFHGR TFRDVVMEPT RIYVKPLLAL 250 260 270 280 290 300 MQAMPGVVKG MAHITGGGIT ENVPRILRDE LTARIDAASW TLPPLFQWLQ QAGNVDTQEM 310 320 330 340 350 YRVFNCGIGM AVVVAADVAD AVAAQLTAAG ETVYRIGRID SRKDGEAQTI VA

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM406670 Genomic DNA. Translation: CAL95753.1.
RefSeq	YP_934639.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1KA97.
Genome annotation databases
GeneID	4607812.
GenomeReviews	Gene locus azo3136 in contig AM406670_GR.
KEGG	azo:azo3136.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VHGLAHI.
Family and domain databases
HAMAP	MF_00741. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR004733. PurM_cligase. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_AZOSB

Accession

Primary (citable) accession number: A1KA97

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)