Reviewed, UniProtKB/Swiss-Prot A1K9V0 (KATG_AZOSB)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase
      Short name=CP
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	azo2989

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	733 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Domain	Signal
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 733	710	Catalase-peroxidase HAMAP MF_01961		PRO_0000354722
Sites
	Active site	97	1	Proton acceptor By similarity
	Metal binding	265	1	Iron (heme axial ligand) By similarity
	Site	93	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	96 ↔ 224		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-250) By similarity
	Cross-link	224 ↔ 250		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-96) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1K9V0-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: E717CCC94DF1CE4E	FASTA	733	80,011
	10 20 30 40 50 60 MNNESKCPFA AAHGVRSPAT ARANRDWWPN QLNLNILHQH APASNPLGED FDYAAEFNTL 70 80 90 100 110 120 DLAALKQDLY ALMTMSQDWW PADWGHYGGL FIRMAWHSAG TYRTADGRGG GGTGNQRFAP 130 140 150 160 170 180 LNSWPDNGNL DKARRLLWPI KQKYGNKISW ADLMILAGNC ALESMGFKTF GFGGGRADIW 190 200 210 220 230 240 QPEEDIYWGA EKEWLATSDK PDSRYSGERQ LENPLAAVQM GLIYVNPEGP DGNPDPVASG 250 260 270 280 290 300 RDVRETFARM AMNDEETVAL VAGGHTFGKA HGAGDPKLVG PEPEGAPIEA QGLGWINSFG 310 320 330 340 350 360 TGHGVHTTTS GIEGAWKPNP TKWDNGYFDM LFGYEWALTR SPAGAHQWVA QNVKPEDMIP 370 380 390 400 410 420 DAHDPSKKHP PMMTTADLSL RFDPIYEPIA RRFHKNPADF ADAFARAWFK LTHRDMGPKS 430 440 450 460 470 480 RYLGPEVPAE DLIWQDPIPR LDHPLIDAAD IATLKAMVLA SGVSIAELVS TAWASASTFR 490 500 510 520 530 540 GSDMRGGANG ARIRLAPQKD WDVNQPAQLA KVLSVLGDIQ AGFNAAAPGG KKVSLADLIV 550 560 570 580 590 600 LGGCAAVEAA ARAAGHVVEV PFTPGRMDAS QEQTDVESFA VLEPIADGFR NYQKQVYAVS 610 620 630 640 650 660 AEELLVDKAQ LLTLSAPEMT VLVGGLRVLG ANAGGSPHGV FTRRPQTLSN DFFVNLLDMS 670 680 690 700 710 720 TAWKPAGDSF EGRDRKSGEL KWTATRVDLV FGSNSQLRAL AEVYAQDDAK EKFVRDFVAA 730 WNKVMNLDRF DVK

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM406670 Genomic DNA. Translation: CAL95605.1.
RefSeq	YP_934492.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1K9V0.
Genome annotation databases
GeneID	4608482.
GenomeReviews	Gene locus azo2989 in contig AM406670_GR.
KEGG	azo:azo2989.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	KTGEPRW.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_AZOSB

Accession

Primary (citable) accession number: A1K9V0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)