A1K9L5 (AMPA_AZOSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable cytosol aminopeptidase
EC=3.4.11.1

Alternative name(s):
Leucine aminopeptidase
Short name=LAP
EC=3.4.11.10
Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	azo2904

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181 Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 500	500	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_1000019879
Sites
	Active site	280	1	Potential
	Active site	354	1	Potential
	Metal binding	268	1	Manganese 2 By similarity
	Metal binding	273	1	Manganese 1 By similarity
	Metal binding	273	1	Manganese 2 By similarity
	Metal binding	291	1	Manganese 2 By similarity
	Metal binding	350	1	Manganese 1 By similarity
	Metal binding	352	1	Manganese 1 By similarity
	Metal binding	352	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1K9L5 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: A3418B04A52A721E	FASTA	500	52,409
	10 20 30 40 50 60 MEFTIKTATP EKVRTGVLVV GLFADGILPP AAHAVDKASK GKLAQLVKRG DLDEKAGAAV 70 80 90 100 110 120 MLHDLAGIAA ERVLVVSLGK SAEFSDKAYR DALNSVAKAL ASGVAKVAAV AIADAELDGR 130 140 150 160 170 180 TLAWRLQQAT RQIADGAYRF DAPKAGAKDA KKERGARKIT LLIGDKVSDE LEAAVRRGQA 190 200 210 220 230 240 VAEGMALAKD LGNLPGNVCT PAYLADTAEA LGKQFKFDVE VLERADMEKL GMGSLLSVAK 250 260 270 280 290 300 GSHIPPKFIV MHYKGGKSKA KPVVLVGKGI TFDTGGISLK PGAEMDEMKY DMCGAASVLG 310 320 330 340 350 360 TFKAIARMAL PLNVVGIVPA TENMPGGNAT RPGDVVTSMS GQTIEILNTD AEGRLILCDA 370 380 390 400 410 420 LTYAERFKPA CVIDIATLTG ACVVALGKIP SGLLANDDDL AAELLKRGTE SGDRAWQLPL 430 440 450 460 470 480 WDEYQDLLKS NFADMANIGG RYGGTITAAC FLARFTKAYK WAHLDIAGTA WVSGDAKGAT 490 500 GRPVPLLAEF LVGRAAAKAD

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BH72.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM406670 Genomic DNA. Translation: CAL95520.1.
RefSeq	YP_934407.1. NC_008702.1.
3D structure databases
ProteinModelPortal	A1K9L5.
ModBase	Search...
Protein-protein interaction databases
STRING	A1K9L5.
Protein family/group databases
MEROPS	M17.003.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4607676.
GenomeReviews	Gene locus azo2904 in contig AM406670_GR.
KEGG	azo:azo2904.
PATRIC	21014230. VBIAzoSp26047_2944.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0260.
HOGENOM	HBG742580.
OMA	KYDWAHL.
PhylomeDB	A1K9L5.
ProtClustDB	PRK00913.
Enzyme and pathway databases
BioCyc	ASP62928:AZO2904-MONOMER.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
KO	K01255.
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_AZOSB

Accession

Primary (citable) accession number: A1K9L5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program