A1K9F0 (PGK_AZOSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoglycerate kinase
EC=2.7.2.3

Gene names

Name:	pgk
Ordered Locus Names:	azo2839

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP MF_00145
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP MF_00145
Subunit structure	Monomer By similarity. HAMAP MF_00145
Subcellular location	Cytoplasm Potential HAMAP MF_00145.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	Phosphoglycerate kinase HAMAP MF_00145		PRO_1000057961
Regions
	Nucleotide binding	347 – 350	4	ATP By similarity
	Region	21 – 23	3	Substrate binding By similarity
	Region	60 – 63	4	Substrate binding By similarity
Sites
	Binding site	37	1	Substrate By similarity
	Binding site	115	1	Substrate By similarity
	Binding site	148	1	Substrate By similarity
	Binding site	199	1	ATP By similarity
	Binding site	321	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1K9F0 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 35854A5292CEB804	FASTA	394	41,548
	10 20 30 40 50 60 MQVKKLTDLD VRGKKVFIRA DLNVPQDEAG NITEDTRIRA SIPSIQYCLD NGAAVMVTSH 70 80 90 100 110 120 LGRPTEGEVG PDDTLAPVAV RLGQLLHKPV KLIANWVEGG FEVKPGEVVL LENCRCNKGE 130 140 150 160 170 180 KKDNEELAKK MAALCDVYVN DAFGTAHRAE ATTHGIARFA PVSCAGILMG AEIDALSKAL 190 200 210 220 230 240 HQPARPLVAI VGGAKVSTKL TILKTLADKV DQLIVGGGIA NTFLLAAGKR IGESLAEPEM 250 260 270 280 290 300 VREAQQVMDI MKSRGAEVPL PIDVVVADEV SALARANRIS VDEVGPHDRI LDFGPKTSAK 310 320 330 340 350 360 LADIIAHAGT IVWNGPVGVF EHAQFAGGTK MMASAIAHSE AFSIAGGGDT LAAIAKFHIA 370 380 390 DDVGYISTGG GAFLEFLEGK KLPAIAALEA RYEG

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BH72.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM406670 Genomic DNA. Translation: CAL95455.1.
RefSeq	YP_934342.1. NC_008702.1.
3D structure databases
ProteinModelPortal	A1K9F0.
SMR	A1K9F0. Positions 3-393.
ModBase	Search...
Protein-protein interaction databases
STRING	A1K9F0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4607640.
GenomeReviews	Gene locus azo2839 in contig AM406670_GR.
KEGG	azo:azo2839.
PATRIC	21014098. VBIAzoSp26047_2878.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0126.
HOGENOM	HBG453500.
OMA	DCIGDEV.
PhylomeDB	A1K9F0.
ProtClustDB	PRK00073.
Enzyme and pathway databases
BioCyc	ASP62928:AZO2839-MONOMER.
Family and domain databases
HAMAP	MF_00145. Phosphoglyc_kinase. [Tree]
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
KO	K00927.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PIRSF	PIRSF000724. Pgk. 1 hit.
PRINTS	PR00477. PHGLYCKINASE.
SUPFAM	SSF53748. PGK. 1 hit.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGK_AZOSB

Accession

Primary (citable) accession number: A1K9F0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program