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Protein

Biotin synthase

Gene

bioB

Organism
Azoarcus sp. (strain BH72)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.
  • [2Fe-2S] clusterNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi66Iron-sulfur 1 (4Fe-4S-S-AdoMet)1
Metal bindingi70Iron-sulfur 1 (4Fe-4S-S-AdoMet)1
Metal bindingi73Iron-sulfur 1 (4Fe-4S-S-AdoMet)1
Metal bindingi110Iron-sulfur 2 (2Fe-2S)1
Metal bindingi141Iron-sulfur 2 (2Fe-2S)1
Metal bindingi201Iron-sulfur 2 (2Fe-2S)1
Metal bindingi273Iron-sulfur 2 (2Fe-2S)1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:azo2817
OrganismiAzoarcus sp. (strain BH72)
Taxonomic identifieri62928 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesZoogloeaceaeAzoarcus
Proteomesi
  • UP000002588 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003812121 – 326Biotin synthaseAdd BLAST326

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi62928.azo2817.

Structurei

3D structure databases

ProteinModelPortaliA1K9C8.
SMRiA1K9C8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.

Phylogenomic databases

eggNOGiENOG4105CZF. Bacteria.
COG0502. LUCA.
HOGENOMiHOG000239957.
KOiK01012.
OMAiADRFCMG.
OrthoDBiPOG091H01DF.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR034416. BATS_domain_containing.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
PANTHERiPTHR22976. PTHR22976. 1 hit.
PfamiView protein in Pfam
PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SFLDiSFLDF00272. biotin_synthase. 1 hit.
SFLDG01060. BATS_domain_containing. 1 hit.
SFLDG01278. biotin_synthase_like. 1 hit.
SFLDS00029. Radical_SAM. 1 hit.
SMARTiView protein in SMART
SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A1K9C8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTTLTAPTP AAAEAPRKKW TVAEVQALFD LPFLDLVFQA QQVHRAHFAA
60 70 80 90 100
NEVQRSTLLS IKTGGCSEDC GYCSQSARYD TGLERERLLP LDEVLEHARA
110 120 130 140 150
AKAKGSSRFC MGAAWRGPKD KDMAPVLEMV REVKKLGLET CVTLGMLKEG
160 170 180 190 200
QAEQLAEAGL DYYNHNIDTA PEFYGQIVTT HTLQDRLDTL DKVRDAGINV
210 220 230 240 250
CCGGIVGMGE TQTSRAGLIA QLANMTPPPD SVPINNLVAI PGTPLADGGT
260 270 280 290 300
IDGFDFVRTI AAARITMPTS YVRLSAGRQQ MSDEMQALCF MAGANSIFYG
310 320
ERLLTTDNPE SDRDDVLFAR LGLRSV
Length:326
Mass (Da):35,406
Last modified:February 6, 2007 - v1
Checksum:iCEDE2D30F6280D98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM406670 Genomic DNA. Translation: CAL95433.1.

Genome annotation databases

EnsemblBacteriaiCAL95433; CAL95433; azo2817.
KEGGiazo:azo2817.

Similar proteinsi

Entry informationi

Entry nameiBIOB_AZOSB
AccessioniPrimary (citable) accession number: A1K9C8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: February 6, 2007
Last modified: November 22, 2017
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families