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A1K9C8

- BIOB_AZOSB

UniProt

A1K9C8 - BIOB_AZOSB

Protein

Biotin synthase

Gene

bioB

Organism
Azoarcus sp. (strain BH72)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 1 (06 Feb 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi70 – 701Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi110 – 1101Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi141 – 1411Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi201 – 2011Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi273 – 2731Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:azo2817
    OrganismiAzoarcus sp. (strain BH72)
    Taxonomic identifieri62928 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAzoarcus
    ProteomesiUP000002588: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 326326Biotin synthasePRO_0000381212Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi62928.azo2817.

    Structurei

    3D structure databases

    ProteinModelPortaliA1K9C8.
    SMRiA1K9C8. Positions 17-323.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiDETQALC.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A1K9C8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTTLTAPTP AAAEAPRKKW TVAEVQALFD LPFLDLVFQA QQVHRAHFAA    50
    NEVQRSTLLS IKTGGCSEDC GYCSQSARYD TGLERERLLP LDEVLEHARA 100
    AKAKGSSRFC MGAAWRGPKD KDMAPVLEMV REVKKLGLET CVTLGMLKEG 150
    QAEQLAEAGL DYYNHNIDTA PEFYGQIVTT HTLQDRLDTL DKVRDAGINV 200
    CCGGIVGMGE TQTSRAGLIA QLANMTPPPD SVPINNLVAI PGTPLADGGT 250
    IDGFDFVRTI AAARITMPTS YVRLSAGRQQ MSDEMQALCF MAGANSIFYG 300
    ERLLTTDNPE SDRDDVLFAR LGLRSV 326
    Length:326
    Mass (Da):35,406
    Last modified:February 6, 2007 - v1
    Checksum:iCEDE2D30F6280D98
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM406670 Genomic DNA. Translation: CAL95433.1.
    RefSeqiWP_011766543.1. NC_008702.1.
    YP_934320.1. NC_008702.1.

    Genome annotation databases

    EnsemblBacteriaiCAL95433; CAL95433; azo2817.
    GeneIDi4608511.
    KEGGiazo:azo2817.
    PATRICi21014052. VBIAzoSp26047_2855.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM406670 Genomic DNA. Translation: CAL95433.1 .
    RefSeqi WP_011766543.1. NC_008702.1.
    YP_934320.1. NC_008702.1.

    3D structure databases

    ProteinModelPortali A1K9C8.
    SMRi A1K9C8. Positions 17-323.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 62928.azo2817.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAL95433 ; CAL95433 ; azo2817 .
    GeneIDi 4608511.
    KEGGi azo:azo2817.
    PATRICi 21014052. VBIAzoSp26047_2855.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi DETQALC.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: BH72.

    Entry informationi

    Entry nameiBIOB_AZOSB
    AccessioniPrimary (citable) accession number: A1K9C8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 54 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3