A1K991 (SYA_AZOSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	azo2780

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	885 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_B
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_B
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_B.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_B
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 885	885	Alanine--tRNA ligase HAMAP MF_00036_B		PRO_0000347495
Sites
	Metal binding	575	1	Zinc Potential
	Metal binding	579	1	Zinc Potential
	Metal binding	676	1	Zinc Potential
	Metal binding	680	1	Zinc Potential

Sequences

	Sequence		Length	Mass (Da)
	A1K991 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: A5615D9260C64B52	FASTA	885	95,745
	10 20 30 40 50 60 MKSAEIRAKF LKFFESKGHQ IVASSSLVPH EDPTLLFTNA GMNQFKDVFL GFDKRPYSRA 70 80 90 100 110 120 TTSQKCVRAG GKHNDLENVG YTARHHTFFE MLGNFSFGDY FKRDAITYAW ELLTEVFKLP 130 140 150 160 170 180 KDKLWVTVYA EDDEAYDIWT KEVGVPAERV IRIGDNKGAR YASDNFWMMG DTGPCGPCTE 190 200 210 220 230 240 IFYDHGPHIW GGPPGSPEED GDRYIEIWNN VFMQFNRDEQ GVMHPLPKPS VDTGMGLERI 250 260 270 280 290 300 SAVLQGVHAN YEIDLFKSLI SSAMRAVGTA EALVERARDS MTNTISPSYK VLADHIRACS 310 320 330 340 350 360 FLIADGVIPG NEGRGYVLRR IIRRAIRHGY KLGARAAFFH KMVPDLVAEM GEAYPELKAG 370 380 390 400 410 420 ERRVADVLRQ EEERFFATIE NGMAIVEGEL AAMATAGNTV FNGDTAFKLH DTYGFPLDLT 430 440 450 460 470 480 ADICREREVT VDAAAFDAAM ARQKEQARAA GKFKMAANLD YDGPATTFHG YDKLEAKGNI 490 500 510 520 530 540 LALYKDGVAV NELVEGDLGV VVLDHTPFYA ESGGQAGDRG ELKGAAGIFG VEDTLKIQAS 550 560 570 580 590 600 VFGHHGVVKT GKLAVGQGVA ARVDTAARAA TARNHSVTHL MHKALREVLG PHVQQKGSLV 610 620 630 640 650 660 DPDKTRFDFA HTAPMSAEEI REVEEIVNRE ILANAATEAA VMALDDAQKS GAMMLFGEKY 670 680 690 700 710 720 GDEVRVLSIG SSKELCGGTH VARTGDIGLF KIVVEGGVAA GVRRVEAVTG ENALRYTQEQ 730 740 750 760 770 780 ERRVQGVSAL LKVQPDEVAE RVAGILDNVR ALEKELARLK SKLAASQGED LVAQAVDVGG 790 800 810 820 830 840 AKLLAATLEG ADVPTLRETM DKLKDKLKSA AIVLASVADG KVSLIAGVTA DLTGKVKAGE 850 860 870 880 LVNFVAQQVG GKGGGRPDMA QAGGTEPDKL PAALAAVQAW VSAKL

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BH72.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM406670 Genomic DNA. Translation: CAL95396.1.
RefSeq	YP_934283.1. NC_008702.1.
3D structure databases
ProteinModelPortal	A1K991.
ModBase	Search...
Protein-protein interaction databases
STRING	A1K991.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4607616.
GenomeReviews	Gene locus azo2780 in contig AM406670_GR.
KEGG	azo:azo2780.
PATRIC	21013978. VBIAzoSp26047_2818.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0013.
HOGENOM	HBG354397.
OMA	MFTNSGM.
PhylomeDB	A1K991.
ProtClustDB	PRK00252.
Enzyme and pathway databases
BioCyc	ASP62928:AZO2780-MONOMER.
Family and domain databases
HAMAP	MF_00036_B. Ala_tRNA_synth_B. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR023033. Ala_tRNA_synth_euk/bac. IPR003156. Pesterase_DHHA1. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF02272. DHHA1. 1 hit. PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_AZOSB

Accession

Primary (citable) accession number: A1K991

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program