Reviewed, UniProtKB/Swiss-Prot A1K976 (SYP_AZOSB)

Names and origin

Protein names

Recommended name:
    Prolyl-tRNA synthetase
    EC=6.1.1.15
Alternative name(s):
    Proline--tRNA ligase
      Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	azo2765

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	581 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS By similarity.
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01569
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Domain	Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain By similarity.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP proline-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 581	581	Prolyl-tRNA synthetase HAMAP MF_01569		PRO_0000288311

Sequences

	Sequence		Length	Mass (Da)
	A1K976-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 1C429A108267C927	FASTA	581	64,025
	10 20 30 40 50 60 MRARQFFIST LKEAPADAEV VSQKLMLRAG MIRKVAAGIY NYLPMGLKSI RKVENIIREE 70 80 90 100 110 120 MNRAGALELT MPLVQPSELW QETGRWDKMG PEMLRFKDRH ERDFALQPTS EEVVTDIARQ 130 140 150 160 170 180 ELKSYRQLPK NFYQIQTKFR DERRPRFGVM RGREFTMKDA YSFDRSAEAA GESYDAMYAA 190 200 210 220 230 240 YSRIFDRLGL TYRAVAADTG AIGGDRSHEF QVIADTGEDA IVYCPDSSYA ANIELAEALS 250 260 270 280 290 300 LLPARAGASA PLAKTPTPGK STCEDVAALL GVPLASTVKS LVLATDDEDE SGKVVKSTVW 310 320 330 340 350 360 LLLVRGDHEL NEVKAGKIEG LKAGFRFATE AEILDHFGCK PGYLGPIGLA QPVKVIADRT 370 380 390 400 410 420 VANMADFVCG ANAEDFHYTG ANWGRDLPEP DLVADIRNVV EGDPSPDGKG KLAIQRGIEV 430 440 450 460 470 480 GHVFYLGTKY SKAMNATFLD VDGKPKHFEM GCYGIGVTRI LGAAIEQNHD ERGIIWPASI 490 500 510 520 530 540 APFEVVICPV GWSKSEAVRD EAQKLYDALV AAGVDVILDD RDERPGVMFA DWELIGVPHR 550 560 570 580 VTIGDRGLKE GVAEYQGRRD PEASRVAVAE IAPHLISRLR P

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM406670 Genomic DNA. Translation: CAL95381.1.
RefSeq	YP_934268.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1K976.
Genome annotation databases
GeneID	4607605.
GenomeReviews	Gene locus azo2765 in contig AM406670_GR.
KEGG	azo:azo2765.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VVSHQLM.
Family and domain databases
HAMAP	MF_01569. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR004500. Pro-tRNA-synth_IIa_bac. IPR002316. Pro-tRNA-synth_IIa_cons-reg. IPR007214. YbaK/aa-tRNA-synth-assoc-reg. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF04073. YbaK. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
TIGRFAMs	TIGR00409. proS_fam_II. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_AZOSB

Accession

Primary (citable) accession number: A1K976

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)