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Reviewed, UniProtKB/Swiss-Prot A1K962 (GLPK_AZOSB)

Last modified November 3, 2009. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Glycerol kinase
    EC=2.7.1.30
Alternative name(s):
    ATP:glycerol 3-phosphotransferase
    Glycerokinase
      Short name=GK
Gene names
Name: glpK
Ordered Locus Names: azo2751
OrganismAzoarcus sp. (strain BH72) [Complete proteome] [HAMAP]
Taxonomic identifier62928 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

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Protein attributes

Sequence length496 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Key enzyme in the regulation of glycerol uptake and metabolism By similarity.

Catalytic activity

ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186

Pathway

Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186

Sequence similarities

Belongs to the FGGY kinase family.

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Ontologies

Keywords
   Biological processGlycerol metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processglycerol-3-phosphate metabolic process

Inferred from electronic annotation. Source: HAMAP

   Molecular functionATP binding

Inferred from electronic annotation. Source: HAMAP

glycerol kinase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 496496Glycerol kinase HAMAP MF_00186
PRO_1000098713

Regions

Nucleotide binding409 – 4135ATP By similarity

Sites

Binding site111Substrate By similarity
Binding site151ATP By similarity
Binding site811Substrate By similarity
Binding site1341Substrate By similarity
Binding site2431Substrate By similarity
Binding site2651ATP By similarity
Binding site3081ATP; via carbonyl oxygen By similarity

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Sequences

Sequence LengthMass (Da)Tools
A1K962-1 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 1E93A8B434A8F7B7

FASTA49652,821
        10         20         30         40         50         60 
MSYVLALDQG TTSSRAMVFD AAGRVRSVAR RDFTQSFPQP GWVEHDPAEI LRSQFDCARD 

        70         80         90        100        110        120 
ALADAGLRAG QLAAIGIANQ RETTVLWERA SGRALAPAIV WQDRRTAAKC AELRARGVWS 

       130        140        150        160        170        180 
EALRERTGLE LDPYFSATKL AWLLDHVPDA RRRAEAGELA FGTVDSWLVW HLSGGRLHVT 

       190        200        210        220        230        240 
DHGNAARTLL LDIHRRDWDD ALLALFDIPR AVLPRLVDSI GVCGESDPAV LGAAVPIAGI 

       250        260        270        280        290        300 
AGDQQAATFG QHCFAPGMAK NTYGTGCFLL MNTGATPVKS SHRLLSTVGW TQGAAHTYAL 

       310        320        330        340        350        360 
EGSIFMGGAT VQWLRDGLGL IREAAEVEAL AARVPDSGGV VLVPAFTGLG APYWDAAARG 

       370        380        390        400        410        420 
ALFGLTRGSG PAHIARAALD AIALQTDDLV RAMTADGAGP LHELRVDGGA AANNLLMQLQ 

       430        440        450        460        470        480 
ADMLGVAVVR PRMLETTALG AACLAGIGAG VWRDTDALAP HWQIDRVFTP AWSEDQRGAV 

       490 
RARWADAVAR SRAWAA

« Hide

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References

[1]"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72."
Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. expand/collapse author list , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A.
Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

AM406670 Genomic DNA. Translation: CAL95367.1.
RefSeqYP_934254.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGA1K962.

Genome annotation databases

GeneID4608911.
GenomeReviewsGene locus azo2751 in contig AM406670_GR.
KEGGazo:azo2751.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAKMAGSAQ.

Family and domain databases

HAMAPMF_00186.
[Tree]
InterProIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PANTHERPTHR10196. FGGY_kin. 1 hit.
PTHR10196:SF9. Glycerol_kin. 1 hit.
PfamPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01311. glycerol_kin. 1 hit.
PROSITEPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameGLPK_AZOSB
AccessionPrimary (citable) accession number: A1K962
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: February 6, 2007
Last modified: November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents