Reviewed, UniProtKB/Swiss-Prot A1K8P4 (HEMH_AZOSB)

Names and origin

Protein names

Recommended name:
    Ferrochelatase
    EC=4.99.1.1
Alternative name(s):
    Protoheme ferro-lyase
    Heme synthetase

Gene names

Name:	hemH
Ordered Locus Names:	azo2582

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX By similarity. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity HAMAP MF_00323.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Ferrochelatase HAMAP MF_00323		PRO_1000079190
Sites
	Metal binding	209	1	Iron By similarity
	Metal binding	290	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1K8P4-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: A355EC9812CC480E	FASTA	363	40,511
	10 20 30 40 50 60 MARFWTEAPH RHGSTARTGI LLVNLGTPVA PTAAALRPYL QQFLSDPRVV EIPRAVWLPL 70 80 90 100 110 120 LNGVILNTRP RKSAAKYASI WTDEGSPLAV HTRRQAELIA ARFASRDDVR VDWAMRYGAP 130 140 150 160 170 180 AVADKLGALR AAGCTRILVV PMYPQYAAST TASVMDEVAR CLQHWRNLPE LRFVRSFHDD 190 200 210 220 230 240 PGYIGALAAS VREHWTRHGQ PDRLLMSFHG LPRYTLERGD PYHCECHRTA RLLAESLGLA 250 260 270 280 290 300 PERVIVSFQS RFGRTRWLEP YTQPTLEALA RDGVGRVDVM CPGFVADCLE TLEEIAMECR 310 320 330 340 350 360 AAFLGAGGRE FHYIPCLNER PDWIDALERR VRSEAGNWLT ATPPTDAERE AARGRALALG AAE

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM406670 Genomic DNA. Translation: CAL95199.1.
RefSeq	YP_934086.1.
3D structure databases
SMR	A1K8P4. Positions 15-339.
ModBase	Search...
Protein-protein interaction databases
STRING	A1K8P4.
Genome annotation databases
GeneID	4609028.
GenomeReviews	Gene locus azo2582 in contig AM406670_GR.
KEGG	azo:azo2582.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0276.
HOGENOM	HBG697135.
OMA	IRPAKSA.
PhylomeDB	A1K8P4.
Family and domain databases
HAMAP	MF_00323. Ferrochelatase. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. hemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_AZOSB

Accession

Primary (citable) accession number: A1K8P4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	February 6, 2007
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)