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A1K7M2 (PYRD_AZOSB) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydroorotate dehydrogenase (quinone)
EC=1.3.5.2
Alternative name(s):
DHOdehase
Short name=DHOD
Short name=DHODase
Dihydroorotate oxidase
Gene names
Name:pyrD
Ordered Locus Names:azo2210
OrganismAzoarcus sp. (strain BH72) [Complete proteome] [HAMAP]
Taxonomic identifier62928 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length338 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor By similarity. HAMAP MF_00225

Catalytic activity

(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP MF_00225

Cofactor

Binds 1 FMN per subunit By similarity. HAMAP MF_00225

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP MF_00225

Subunit structure

Monomer By similarity. HAMAP MF_00225

Subcellular location

Cell membrane; Peripheral membrane protein By similarity HAMAP MF_00225.

Sequence similarities

Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
   Biological processPyrimidine biosynthesis
   Cellular componentCell membrane
Membrane
   LigandFMN
Flavoprotein
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological process'de novo' pyrimidine base biosynthetic process

Inferred from electronic annotation. Source: InterPro

UMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydroorotate oxidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 338338Dihydroorotate dehydrogenase (quinone) HAMAP MF_00225
PRO_1000024152

Regions

Nucleotide binding59 – 635FMN By similarity
Nucleotide binding315 – 3162FMN By similarity
Region108 – 1125Substrate binding By similarity
Region243 – 2442Substrate binding By similarity

Sites

Active site1721Nucleophile By similarity
Binding site631Substrate By similarity
Binding site831FMN; via amide nitrogen By similarity
Binding site1361FMN By similarity
Binding site1691FMN By similarity
Binding site1691Substrate By similarity
Binding site1741Substrate By similarity
Binding site2141FMN By similarity
Binding site2421FMN; via carbonyl oxygen By similarity
Binding site2651FMN; via amide nitrogen By similarity
Binding site2941FMN; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
A1K7M2 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: B3028C831DC3CA8B

FASTA33836,234
        10         20         30         40         50         60 
MLYDIARPFL FSLDAETAHE FTLAALNLAG RTLPAGKPEA ADAVRVMGID FPNRIGLAAG 

        70         80         90        100        110        120 
LDKNGEAIDG LARLGFGFIE IGTITPRPQP GNPRPRLFRL PEVRGIINRM GFNNHGVDTL 

       130        140        150        160        170        180 
VANVKAARFK GVLGINIGKN FDTPIENAAD DYLACLDKVY PLASYVTVNI SSPNTKNLRQ 

       190        200        210        220        230        240 
LQGESELDDL LGRLKSAQQR LADQHGRYVP LTLKIAPDLE AAQITNIADA LRRHRIDAVI 

       250        260        270        280        290        300 
ATNTTISRDK VQGVRFAEQQ GGLSGAPVFE ASTAVVAQLA TALGGELPII AAGGVMDTRS 

       310        320        330 
ARAKLEAGAS LVQLYSGLIY RGPCLVRECV RATADFPR

« Hide

References

[1]"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72."
Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. expand/collapse author list , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A.
Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BH72.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM406670 Genomic DNA. Translation: CAL94827.1.
RefSeqYP_933714.1. NC_008702.1.

3D structure databases

ProteinModelPortalA1K7M2.
SMRA1K7M2. Positions 1-332.
ModBaseSearch...

Protein-protein interaction databases

STRINGA1K7M2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4608363.
GenomeReviewsGene locus azo2210 in contig AM406670_GR.
KEGGazo:azo2210.
PATRIC21012830. VBIAzoSp26047_2246.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0167.
HOGENOMHBG351027.
OMASYVTVNI.
PhylomeDBA1K7M2.
ProtClustDBPRK05286.

Enzyme and pathway databases

BioCycASP62928:AZO2210-MONOMER.

Family and domain databases

HAMAPMF_00225. DHO_dh_type2.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR012135. Dihydroorotate_DH_1_2.
IPR005719. Dihydroorotate_DH_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK00226.
PfamPF01180. DHO_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMsTIGR01036. PyrD_sub2. 1 hit.
PROSITEPS00911. DHODEHASE_1. 1 hit.
PS00912. DHODEHASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePYRD_AZOSB
AccessionPrimary (citable) accession number: A1K7M2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: December 14, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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