Reviewed, UniProtKB/Swiss-Prot A1K7J8 (ASSY_AZOSB)

Names and origin

Protein names

Recommended name:
    Argininosuccinate synthase
    EC=6.3.4.5
Alternative name(s):
    Citrulline--aspartate ligase

Gene names

Name:	argG
Ordered Locus Names:	azo2186

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	409 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP MF_00005
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP MF_00005
Subunit structure	Homotetramer By similarity. HAMAP MF_00005
Subcellular location	Cytoplasm Probable HAMAP MF_00005.
Sequence similarities	Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP argininosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 409	409	Argininosuccinate synthase HAMAP MF_00005		PRO_1000000381
Regions
	Nucleotide binding	10 – 18	9	ATP By similarity
Sites
	Binding site	37	1	ATP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	90	1	Citrulline By similarity
	Binding site	95	1	Citrulline By similarity
	Binding site	120	1	ATP; via amide nitrogen By similarity
	Binding site	122	1	Aspartate By similarity
	Binding site	126	1	Aspartate By similarity
	Binding site	126	1	Citrulline By similarity
	Binding site	127	1	Aspartate By similarity
	Binding site	130	1	Citrulline By similarity
	Binding site	182	1	Citrulline By similarity
	Binding site	191	1	Citrulline By similarity
	Binding site	267	1	Citrulline By similarity
	Binding site	279	1	Citrulline By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1K7J8-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 06CFBAE9C0F1A32B	FASTA	409	46,055
	10 20 30 40 50 60 MSDVKKVVLA YSGGLDTSVI LKWLQDTYQC EVVTFTADLG QGEELEPARQ KALKFGIKPE 70 80 90 100 110 120 NIFIDDLREE FVRDFVFPMF RANTIYEGEY LLGTSIARPL IAKRQIEIAR ATGADAVSHG 130 140 150 160 170 180 ATGKGNDQVR FELGYYALMP GVKVIAPWRE WDLLSREKLL AYAEKHGIPI EMKHKQGGSP 190 200 210 220 230 240 YSMDANLLHI SFEGRHLENP AAEAEESMWR WTVSPEAAPD AAEYVDLEFE KGDLVAINGQ 250 260 270 280 290 300 RLKAHELLAK LNELGGKHGI GRLDLVENRY VGMKSRGCYE TPGGTILLRA HRAIESITLD 310 320 330 340 350 360 REVAHLKDDL MPRYASMIYN GYWWSPERQA LQALVDHTQQ TVNGWVRIKL YKGNVIVVAR 370 380 390 400 DSKTDSLFDP TIATFEDDQG AYNQKDAHGF IRLNALRMRI AANAKTKRG

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM406670 Genomic DNA. Translation: CAL94803.1.
RefSeq	YP_933690.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1K7J8.
Genome annotation databases
GeneID	4607280.
GenomeReviews	Gene locus azo2186 in contig AM406670_GR.
KEGG	azo:azo2186.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0137.
HOGENOM	HBG335267.
OMA	PLIAKRQ.
PhylomeDB	A1K7J8.
Family and domain databases
HAMAP	MF_00005. Arg_succ_synth_type1. [Tree]
InterPro	IPR001518. Arginosuc_synth. IPR018223. Arginosuc_synth_CS. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11587. Arginosuc_synth. 1 hit.
Pfam	PF00764. Arginosuc_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00032. argG. 1 hit.
PROSITE	PS00564. ARGININOSUCCIN_SYN_1. 1 hit. PS00565. ARGININOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASSY_AZOSB

Accession

Primary (citable) accession number: A1K7J8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	February 9, 2010
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)