A1K776 (LEXA_AZOSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
LexA repressor
EC=3.4.21.88

Gene names

Name:	lexA
Ordered Locus Names:	azo2064

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity. HAMAP MF_00015
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity. HAMAP MF_00015
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW SOS response Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 202	202	LexA repressor HAMAP MF_00015		PRO_1000001257
Regions
	DNA binding	32 – 52	21	H-T-H motif By similarity
Sites
	Active site	121	1	For autocatalytic cleavage activity By similarity
	Active site	158	1	For autocatalytic cleavage activity By similarity
	Site	86 – 87	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1K776 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: B13D7A43761CA120	FASTA	202	22,023
	10 20 30 40 50 60 MNARNEQLTS RQQEILDFIR QTVESEGRPP TRAEVCSAFG FKSPNAAETH LRALAAKGAI 70 80 90 100 110 120 LLEEGRARGI RLAEALGLPL VGRVAAGNPI LAAEHVEARI QFDPALFSPR ADYLLRVRGM 130 140 150 160 170 180 SMRDAGILDG DLIAVHRSHE ARNGQVVVAR IDDDVTVKTL RRNGPIVELL PANPDFDPIV 190 200 VDTRSAALEL EGIMVGLIRT DH

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BH72.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM406670 Genomic DNA. Translation: CAL94681.1.
RefSeq	YP_933568.1. NC_008702.1.
3D structure databases
ProteinModelPortal	A1K776.
SMR	A1K776. Positions 8-199.
ModBase	Search...
Protein-protein interaction databases
STRING	A1K776.
Protein family/group databases
MEROPS	S24.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4608379.
GenomeReviews	Gene locus azo2064 in contig AM406670_GR.
KEGG	azo:azo2064.
PATRIC	21012526. VBIAzoSp26047_2100.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1974.
HOGENOM	HBG679610.
OMA	IAENPDF.
PhylomeDB	A1K776.
ProtClustDB	PRK00215.
Enzyme and pathway databases
BioCyc	ASP62928:AZO2064-MONOMER.
Family and domain databases
HAMAP	MF_00015. LexA. [Tree]
InterPro	IPR006199. LexA_DNA-bd_dom. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. WHTH_trsnscrt_rep_DNA-bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
KO	K01356.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
SUPFAM	SSF51306. Pept_S24_S26_C. 1 hit.
TIGRFAMs	TIGR00498. LexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA_AZOSB

Accession

Primary (citable) accession number: A1K776

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program