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A1K710 (A1K710_AZOSB) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable 6-oxopurine nucleoside phosphorylase HAMAP-Rule MF_01963
EC=2.4.2.1 HAMAP-Rule MF_01963
Alternative name(s):
Purine nucleoside phosphorylase HAMAP-Rule MF_01963
Gene names
Ordered Locus Names:azo1998 EMBL CAL94615.1
OrganismAzoarcus sp. (strain BH72) [Complete proteome] [HAMAP] EMBL CAL94615.1
Taxonomic identifier62928 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length246 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Purine nucleoside phosphorylase which is highly specific for 6-oxopurine nucleosides. Cleaves guanosine or inosine to respective bases and sugar-1-phosphate molecules. Involved in purine salvage By similarity. HAMAP-Rule MF_01963

Catalytic activity

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate. HAMAP-Rule MF_01963

Pathway

Purine metabolism; purine nucleoside salvage. HAMAP-Rule MF_01963

Subunit structure

Homohexamer. Dimer of a homotrimer By similarity. HAMAP-Rule MF_01963

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines By similarity. HAMAP-Rule MF_01963

Sequence similarities

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily. HAMAP-Rule MF_01963

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region50 – 512Phosphate binding By similarity HAMAP-Rule MF_01963
Region207 – 2093Substrate binding By similarity HAMAP-Rule MF_01963

Sites

Binding site81Phosphate By similarity HAMAP-Rule MF_01963
Binding site1831Substrate; via amide nitrogen By similarity HAMAP-Rule MF_01963
Binding site1841Phosphate By similarity HAMAP-Rule MF_01963
Site1651Important for substrate specificity By similarity HAMAP-Rule MF_01963
Site2211Important for substrate specificity By similarity HAMAP-Rule MF_01963

Sequences

Sequence LengthMass (Da)Tools
A1K710 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 1868A9D5B1A59760

FASTA24626,490
        10         20         30         40         50         60 
MLAIIGGSGL TQLSTLEVVR REVCRTPYGE PSGALTFGTL AGKPVVFLAR HGYGHTIPPH 

        70         80         90        100        110        120 
LVNYRANIWA LHQARATAVV SVASVGGIRA DFAPGTLAVP DQIIDYTWGR KNTFFDGGEA 

       130        140        150        160        170        180 
PVRHIDFTHP YDEKLRRSLL AAATDAGEPI FDGGVYASMQ GPRLETAAEI NRLERDGADL 

       190        200        210        220        230        240 
VGMTGMPEAA LARELDLPYA ALNVVVNYAA GRSSSEHGIH FDSIELVLQE AMLRVRRVLE 


ALCRAG

« Hide

References

[1]"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72."
Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. expand/collapse author list , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A.
Nat. Biotechnol. 24:1385-1391(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BH72 EMBL CAL94615.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM406670 Genomic DNA. Translation: CAL94615.1.
RefSeqYP_933502.1. NC_008702.1.

3D structure databases

ProteinModelPortalA1K710.
ModBaseSearch...

Protein-protein interaction databases

STRING62928.azo1998.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL94615; CAL94615; azo1998.
GeneID4608374.
KEGGazo:azo1998.
PATRIC21012394. VBIAzoSp26047_2034.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0005.
HOGENOMHOG000228987.
KOK00772.
OMASTFCEEP.
ProtClustDBPRK09136.

Enzyme and pathway databases

UniPathwayUPA00606.

Family and domain databases

HAMAPMF_01963. MTAP.
InterProIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
[Graphical view]
PANTHERPTHR11904. PTHR11904. 1 hit.
PfamPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA1K710_AZOSB
AccessionPrimary (citable) accession number: A1K710
Entry history
Integrated into UniProtKB/TrEMBL: February 6, 2007
Last sequence update: February 6, 2007
Last modified: May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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