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Protein

2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase

Gene

mhpB

Organism
Azoarcus sp. (strain BH72)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively.UniRule annotation

Catalytic activityi

3-(2,3-dihydroxyphenyl)propanoate + O2 = (2Z,4E)-2-hydroxy-6-oxonona-2,4-diene-1,9-dioate.UniRule annotation
(2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + O2 = (2Z,4E,7E)-2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: 3-phenylpropanoate degradation

This protein is involved in the pathway 3-phenylpropanoate degradation, which is part of Aromatic compound metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway 3-phenylpropanoate degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei115 – 1151Proton donorUniRule annotation
Active sitei179 – 1791Proton acceptorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Iron

Enzyme and pathway databases

UniPathwayiUPA00714.

Names & Taxonomyi

Protein namesi
Recommended name:
2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenaseUniRule annotation (EC:1.13.11.16UniRule annotation)
Alternative name(s):
3-carboxyethylcatechol 2,3-dioxygenaseUniRule annotation
Gene namesi
Name:mhpBUniRule annotation
Ordered Locus Names:azo1975
OrganismiAzoarcus sp. (strain BH72)
Taxonomic identifieri62928 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAzoarcus
Proteomesi
  • UP000002588 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3173172,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenasePRO_0000337644Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi62928.azo1975.

Structurei

3D structure databases

ProteinModelPortaliA1K6Y7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LigB/MhpB extradiol dioxygenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105MI0. Bacteria.
ENOG410XPPS. LUCA.
HOGENOMiHOG000069851.
KOiK05713.
OMAiHCLSHTP.
OrthoDBiEOG62ZHSQ.

Family and domain databases

Gene3Di3.40.830.10. 2 hits.
HAMAPiMF_01653. MhpB.
InterProiIPR023789. DHPP/DHXA_dioxygenase.
IPR004183. Xdiol_dOase_suB.
[Graphical view]
PfamiPF02900. LigB. 1 hit.
[Graphical view]
SUPFAMiSSF53213. SSF53213. 2 hits.

Sequencei

Sequence statusi: Complete.

A1K6Y7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAMLQCMSH TPLKGYFDPA ADVVEEVAAL AAAVREEVAH FDPEVVYLFA
60 70 80 90 100
PDHYNGFFLD LMPQFCLGIE ATSVGDYQTP KGPLRVPRAL AEACAGAVIA
110 120 130 140 150
RGIDLAFSYR MQVDHGFAQT LVEVLGGLAR YPVVPIMINA VAPPLASFQR
160 170 180 190 200
ARLLGAAVGE FARGQGGRAL FIASGGMSHN PPIPQIATAT DPEVIERIVA
210 220 230 240 250
GRNPTPEARA ARQQRTVAAA QAFAAGDSPL RPLNPDWDRA LMKRLCSRDW
260 270 280 290 300
AAIDAYRNDE VSAAAGASAH ELKSWVAATA AMESATAGQW DARERYYRAI
310
PEWLAGFGAM SGLGRHP
Length:317
Mass (Da):33,945
Last modified:February 6, 2007 - v1
Checksum:i8639B236B996ECCD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM406670 Genomic DNA. Translation: CAL94592.1.
RefSeqiWP_011765708.1. NC_008702.1.

Genome annotation databases

EnsemblBacteriaiCAL94592; CAL94592; azo1975.
KEGGiazo:azo1975.
PATRICi21012342. VBIAzoSp26047_2008.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM406670 Genomic DNA. Translation: CAL94592.1.
RefSeqiWP_011765708.1. NC_008702.1.

3D structure databases

ProteinModelPortaliA1K6Y7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi62928.azo1975.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL94592; CAL94592; azo1975.
KEGGiazo:azo1975.
PATRICi21012342. VBIAzoSp26047_2008.

Phylogenomic databases

eggNOGiENOG4105MI0. Bacteria.
ENOG410XPPS. LUCA.
HOGENOMiHOG000069851.
KOiK05713.
OMAiHCLSHTP.
OrthoDBiEOG62ZHSQ.

Enzyme and pathway databases

UniPathwayiUPA00714.

Family and domain databases

Gene3Di3.40.830.10. 2 hits.
HAMAPiMF_01653. MhpB.
InterProiIPR023789. DHPP/DHXA_dioxygenase.
IPR004183. Xdiol_dOase_suB.
[Graphical view]
PfamiPF02900. LigB. 1 hit.
[Graphical view]
SUPFAMiSSF53213. SSF53213. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BH72.

Entry informationi

Entry nameiMHPB_AZOSB
AccessioniPrimary (citable) accession number: A1K6Y7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 6, 2007
Last modified: December 9, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.