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A1K6Y7 (MHPB_AZOSB) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
2,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase
EC=1.13.11.16
Gene names
Name:mhpB
Ordered Locus Names:azo1975
OrganismAzoarcus sp. (strain BH72) [Complete proteome] [HAMAP]
Taxonomic identifier62928 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively By similarity. HAMAP MF_01653

Catalytic activity

3-(2,3-dihydroxyphenyl)propanoate + O2 = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate. HAMAP MF_01653

(2E)-3-(2,3-dihydroxyphenyl)prop-2-enoate + O2 = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate. HAMAP MF_01653

Cofactor

Fe2+ ion By similarity. HAMAP MF_01653

Pathway

Aromatic compound metabolism; 3-phenylpropanoate degradation. HAMAP MF_01653

Subunit structure

Homotetramer By similarity. HAMAP MF_01653

Sequence similarities

Belongs to the ligB/mhpB extradiol dioxygenase family.

Ontologies

Keywords
   Biological processAromatic hydrocarbons catabolism
   LigandIron
   Molecular functionDioxygenase
Oxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processaromatic compound catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular function3-carboxyethylcatechol 2,3-dioxygenase activity

Inferred from electronic annotation. Source: EC

ferrous iron binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3173172,3-dihydroxyphenylpropionate/2,3-dihydroxicinnamic acid 1,2-dioxygenase HAMAP MF_01653
PRO_0000337644

Sites

Active site1151Proton donor By similarity
Active site1791Proton acceptor By similarity

Sequences

Sequence LengthMass (Da)Tools
A1K6Y7 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 8639B236B996ECCD

FASTA31733,945
        10         20         30         40         50         60 
MSAMLQCMSH TPLKGYFDPA ADVVEEVAAL AAAVREEVAH FDPEVVYLFA PDHYNGFFLD 

        70         80         90        100        110        120 
LMPQFCLGIE ATSVGDYQTP KGPLRVPRAL AEACAGAVIA RGIDLAFSYR MQVDHGFAQT 

       130        140        150        160        170        180 
LVEVLGGLAR YPVVPIMINA VAPPLASFQR ARLLGAAVGE FARGQGGRAL FIASGGMSHN 

       190        200        210        220        230        240 
PPIPQIATAT DPEVIERIVA GRNPTPEARA ARQQRTVAAA QAFAAGDSPL RPLNPDWDRA 

       250        260        270        280        290        300 
LMKRLCSRDW AAIDAYRNDE VSAAAGASAH ELKSWVAATA AMESATAGQW DARERYYRAI 

       310 
PEWLAGFGAM SGLGRHP

« Hide

References

[1]"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72."
Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. expand/collapse author list , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A.
Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BH72.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AM406670 Genomic DNA. Translation: CAL94592.1.
RefSeqYP_933479.1. NC_008702.1.

3D structure databases

ProteinModelPortalA1K6Y7.
ModBaseSearch...

Protein-protein interaction databases

STRINGA1K6Y7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID4609220.
GenomeReviewsGene locus azo1975 in contig AM406670_GR.
KEGGazo:azo1975.
PATRIC21012342. VBIAzoSp26047_2008.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGNOG42398.
HOGENOMHBG565586.
ProtClustDBPRK13370.

Enzyme and pathway databases

BioCycASP62928:AZO1975-MONOMER.

Family and domain databases

HAMAPMF_01653. MhpB.
[Tree]
InterProIPR023789. DHPP/DHXA_dioxygenase.
IPR004183. Xdiol_dOase_suB.
[Graphical view]
Gene3DG3DSA:3.40.830.10. Xdiol_dOase_3B. 1 hit.
KOK05713.
PfamPF02900. LigB. 1 hit.
[Graphical view]
SUPFAMSSF53213. Xdiol_dOase_3B. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMHPB_AZOSB
AccessionPrimary (citable) accession number: A1K6Y7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: February 6, 2007
Last modified: January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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