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Protein

Malate dehydrogenase

Gene

mdh

Organism
Azoarcus sp. (strain BH72)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei93 – 931SubstrateUniRule annotation
Binding sitei99 – 991SubstrateUniRule annotation
Binding sitei106 – 1061NADUniRule annotation
Binding sitei113 – 1131NADUniRule annotation
Binding sitei132 – 1321SubstrateUniRule annotation
Binding sitei166 – 1661SubstrateUniRule annotation
Active sitei191 – 1911Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 187NADUniRule annotation
Nucleotide bindingi130 – 1323NADUniRule annotation

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:mdhUniRule annotation
Ordered Locus Names:azo1547
OrganismiAzoarcus sp. (strain BH72)
Taxonomic identifieri62928 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAzoarcus
ProteomesiUP000002588: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330Malate dehydrogenasePRO_0000294374Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi62928.azo1547.

Structurei

3D structure databases

ProteinModelPortaliA1K5Q9.
SMRiA1K5Q9. Positions 3-330.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000220953.
KOiK00024.
OMAiFVTTVQQ.
OrthoDBiEOG6PP9Q2.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.

Sequencei

Sequence statusi: Complete.

A1K5Q9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKAPVRVAV TGAAGQIGYS LLFRIASGEM LGKDQPVILQ LLDLPQAQKA
60 70 80 90 100
VKGVMMELED CAFPLLAGMI ATDDPNVAFK DAKVALLVGA RPRGPGMERK
110 120 130 140 150
DLLTENAKIF TVQGAAIGQY ADPDCKVLVV GNPCNTNAYI AKEVAQKYGR
160 170 180 190 200
VPAKNFTGML RLDHNRALSQ LAGKSGREVS SLKNLVVWGN HSPTMYADYR
210 220 230 240 250
FVKSNGDSVK DLINDAAWNK DVFLPTVGKR GAAIIEARGL SSAASAANAA
260 270 280 290 300
IDHIRDWVLG SNGEWVTMGI PSDGSYGIPE GVIYGFPVTT ENGEYKIVQG
310 320 330
LEIDEFSRER MTVTLNELLE EREGVKDLLA
Length:330
Mass (Da):35,527
Last modified:February 6, 2007 - v1
Checksum:iDEC4AA51D738E299
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM406670 Genomic DNA. Translation: CAL94164.1.
RefSeqiWP_011765280.1. NC_008702.1.
YP_933051.1. NC_008702.1.

Genome annotation databases

EnsemblBacteriaiCAL94164; CAL94164; azo1547.
GeneIDi4608485.
KEGGiazo:azo1547.
PATRICi21011466. VBIAzoSp26047_1577.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM406670 Genomic DNA. Translation: CAL94164.1.
RefSeqiWP_011765280.1. NC_008702.1.
YP_933051.1. NC_008702.1.

3D structure databases

ProteinModelPortaliA1K5Q9.
SMRiA1K5Q9. Positions 3-330.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi62928.azo1547.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL94164; CAL94164; azo1547.
GeneIDi4608485.
KEGGiazo:azo1547.
PATRICi21011466. VBIAzoSp26047_1577.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000220953.
KOiK00024.
OMAiFVTTVQQ.
OrthoDBiEOG6PP9Q2.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_01517. Malate_dehydrog_2.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BH72.

Entry informationi

Entry nameiMDH_AZOSB
AccessioniPrimary (citable) accession number: A1K5Q9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: February 6, 2007
Last modified: January 7, 2015
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.