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Protein

tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC

Gene

mnmC

Organism
Azoarcus sp. (strain BH72)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the last two steps in the biosynthesis of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm5s2U34 to nm5s2U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm5s2U34, to form mnm5s2U34.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + tRNA containing 5-aminomethyl-2-thiouridine = S-adenosyl-L-homocysteine + tRNA containing 5-methylaminomethyl-2-thiouridylate.UniRule annotation

Cofactori

FADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Oxidoreductase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

FAD, Flavoprotein, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmCUniRule annotation
Short name:
tRNA mnm(5)s(2)U biosynthesis bifunctional proteinUniRule annotation
Including the following 2 domains:
tRNA (mnm(5)s(2)U34)-methyltransferaseUniRule annotation (EC:2.1.1.61UniRule annotation)
FAD-dependent cmnm(5)s(2)U34 oxidoreductaseUniRule annotation (EC:1.5.-.-UniRule annotation)
Gene namesi
Name:mnmCUniRule annotation
Ordered Locus Names:azo1482
OrganismiAzoarcus sp. (strain BH72)
Taxonomic identifieri62928 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAzoarcus
Proteomesi
  • UP000002588 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 635635tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmCPRO_0000347941Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi62928.azo1482.

Structurei

3D structure databases

ProteinModelPortaliA1K5J4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 231231tRNA (mnm(5)s(2)U34)-methyltransferaseAdd
BLAST
Regioni249 – 635387FAD-dependent cmnm(5)s(2)U34 oxidoreductaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the methyltransferase superfamily. tRNA (mnm(5)s(2)U34)-methyltransferase family.UniRule annotation
In the C-terminal section; belongs to the DAO family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CWG. Bacteria.
COG0665. LUCA.
COG4121. LUCA.
HOGENOMiHOG000218143.
KOiK15461.
OMAiKSVLCYD.
OrthoDBiPOG091H04VA.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
3.50.50.60. 3 hits.
HAMAPiMF_01102. MnmC. 1 hit.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR008471. MnmC-like_methylTransf.
IPR029063. SAM-dependent_MTases.
IPR023032. tRNA_MAMT_biosynth_bifunc_MnmC.
IPR017610. tRNA_S-uridine_synth_MnmC_C.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF05430. Methyltransf_30. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR03197. MnmC_Cterm. 1 hit.

Sequencei

Sequence statusi: Complete.

A1K5J4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPITPASLSF AEDGTPYSTA YGDVYHSSDG GLGQAHHVFL AGNGLPERWR
60 70 80 90 100
GRERFVIVET GFGLGLNFLA SWAAWRKDPQ RSERLHFVSC ELHPFRVEDL
110 120 130 140 150
ALLHARWPEF APLAAELQAN WPCLAPGVHR LHLDGGRVCL TLYFGDARDG
160 170 180 190 200
LAQLDARADA FLLDGFSPAK NPDLWSARIF HLLARLAAAD ATLATWSVAG
210 220 230 240 250
EVREGLRRAG FEVEKAPGFG GKRQMLRGRY LGRGHRPAPA AAERRALVIG
260 270 280 290 300
AGVAGTSIAE RLAARGWQVE LLDAASGPAQ GASGNHAGVL RPLPSLDDNR
310 320 330 340 350
MGRLTRAGTL YGWRHIQRLQ AAGLPLRAEA CGVLHLARDA AQEAKMRAVV
360 370 380 390 400
ERLALPPAHL RFVSAAEASE IGGWPVPLGG WWFGDSGWVQ PPSLCAANLS
410 420 430 440 450
AGGEGIRAHW NARVTLARAD TRWQARDAQG ALLAEAPVAI LAAGTGITGF
460 470 480 490 500
PLAAPLPVVS ARGQVSLLPA PAGSAPRVVM CRMGYVSPAV DGLRCAGATF
510 520 530 540 550
DVGDDDATLR ARDHHENLSK LEAMLPGYTA ALATQPAEGR VGFRPASPDR
560 570 580 590 600
LPMVGAVPAL TTLAAPCALA EIPRHTGLYA LSGFGARGLV WATLAAETLA
610 620 630
SQLDGEPLPL ERDLVDALDP ARFLLRPART LRGED
Length:635
Mass (Da):67,530
Last modified:February 6, 2007 - v1
Checksum:i4BDAD1F311BC22F5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM406670 Genomic DNA. Translation: CAL94099.1.
RefSeqiWP_011765215.1. NC_008702.1.

Genome annotation databases

EnsemblBacteriaiCAL94099; CAL94099; azo1482.
KEGGiazo:azo1482.
PATRICi21011330. VBIAzoSp26047_1509.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM406670 Genomic DNA. Translation: CAL94099.1.
RefSeqiWP_011765215.1. NC_008702.1.

3D structure databases

ProteinModelPortaliA1K5J4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi62928.azo1482.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL94099; CAL94099; azo1482.
KEGGiazo:azo1482.
PATRICi21011330. VBIAzoSp26047_1509.

Phylogenomic databases

eggNOGiENOG4105CWG. Bacteria.
COG0665. LUCA.
COG4121. LUCA.
HOGENOMiHOG000218143.
KOiK15461.
OMAiKSVLCYD.
OrthoDBiPOG091H04VA.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
3.50.50.60. 3 hits.
HAMAPiMF_01102. MnmC. 1 hit.
InterProiIPR006076. FAD-dep_OxRdtase.
IPR023753. FAD/NAD-binding_dom.
IPR008471. MnmC-like_methylTransf.
IPR029063. SAM-dependent_MTases.
IPR023032. tRNA_MAMT_biosynth_bifunc_MnmC.
IPR017610. tRNA_S-uridine_synth_MnmC_C.
[Graphical view]
PfamiPF01266. DAO. 1 hit.
PF05430. Methyltransf_30. 1 hit.
[Graphical view]
SUPFAMiSSF51905. SSF51905. 2 hits.
TIGRFAMsiTIGR03197. MnmC_Cterm. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMNMC_AZOSB
AccessioniPrimary (citable) accession number: A1K5J4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: February 6, 2007
Last modified: September 7, 2016
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.