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A1K5C1 (NUON_AZOSB) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit N

EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I subunit N
NDH-1 subunit N
Gene names
Name:nuoN
Ordered Locus Names:azo1409
OrganismAzoarcus sp. (strain BH72) [Complete proteome] [HAMAP]
Taxonomic identifier62928 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAzoarcus

Protein attributes

Sequence length491 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP-Rule MF_00445

Catalytic activity

NADH + quinone = NAD+ + quinol. HAMAP-Rule MF_00445

Subunit structure

NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.

Subcellular location

Cell inner membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00445.

Sequence similarities

Belongs to the complex I subunit 2 family.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandNAD
Ubiquinone
   Molecular functionOxidoreductase
   PTMQuinone
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processATP synthesis coupled electron transport

Inferred from electronic annotation. Source: InterPro

transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNADH dehydrogenase (ubiquinone) activity

Inferred from electronic annotation. Source: InterPro

quinone binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 491491NADH-quinone oxidoreductase subunit N HAMAP-Rule MF_00445
PRO_0000391101

Regions

Transmembrane11 – 3121Helical; Potential
Transmembrane38 – 5821Helical; Potential
Transmembrane74 – 9421Helical; Potential
Transmembrane106 – 12621Helical; Potential
Transmembrane128 – 14821Helical; Potential
Transmembrane163 – 18321Helical; Potential
Transmembrane206 – 22621Helical; Potential
Transmembrane243 – 26321Helical; Potential
Transmembrane272 – 29221Helical; Potential
Transmembrane301 – 32121Helical; Potential
Transmembrane336 – 35621Helical; Potential
Transmembrane379 – 39921Helical; Potential
Transmembrane410 – 43021Helical; Potential
Transmembrane465 – 48521Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
A1K5C1 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 5646C6FF251B4DA8

FASTA49153,128
        10         20         30         40         50         60 
MNFVVPDFYP ATAEIFVAVM ALVIMLATTF ARSVARGLAY GLTLVTLIGA AIITYNTANP 

        70         80         90        100        110        120 
RAVTTFSNMF IGDLLGDVLK LLIYFSMAVA LLYGRSYLAD RKLDKPEYYV LALLMTLGMM 

       130        140        150        160        170        180 
VMVTSNHLLS MYIGLELMSL SLYALVAFDR DSARGTEAAM KYFVLGALAS GLLLYGMSML 

       190        200        210        220        230        240 
YGATGTLEIS GIAKSVYNQA ANDTVLLFGL VFLMAGICFK LGVVPFHMWI PDVYHGANTA 

       250        260        270        280        290        300 
VTLIIATAPK LAAFAMAVRL LVWGLFDVAE HWQTMLMFVA VLSIVLGNLA AIAQTNLKRM 

       310        320        330        340        350        360 
LAYSGISHMG FMLLGLLSGV VDGDPHYALD AYSAAMFYAI SYVIMSLASF GMIILLSRAG 

       370        380        390        400        410        420 
FEAENIDDFK GLNKRSPWFA AMMMFVMFSM AGIPFFIGFF AKLAVLQAVV AAGYIWVAVV 

       430        440        450        460        470        480 
AVLMSVIGAF YYLRLVKVMY FDEPADATPI QAPAELRVFL SANGLAIAAI GLAPQGVMTL 

       490 
CTFVLLASTQ P 

« Hide

References

[1]"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72."
Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. expand/collapse author list , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A.
Nat. Biotechnol. 24:1385-1391(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: BH72.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM406670 Genomic DNA. Translation: CAL94026.1.
RefSeqYP_932913.1. NC_008702.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING62928.azo1409.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL94026; CAL94026; azo1409.
GeneID4609431.
KEGGazo:azo1409.
PATRIC21011180. VBIAzoSp26047_1434.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1007.
HOGENOMHOG000100795.
KOK00343.
OMAMVSSNHL.
OrthoDBEOG64JFNZ.

Family and domain databases

HAMAPMF_00445. NDH1_NuoN_1.
InterProIPR010096. NADH-Q_OxRdtase_suN/2.
IPR001750. NADH_UbQ/plastoQ_OxRdtase.
[Graphical view]
PfamPF00361. Oxidored_q1. 1 hit.
[Graphical view]
TIGRFAMsTIGR01770. NDH_I_N. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNUON_AZOSB
AccessionPrimary (citable) accession number: A1K5C1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 9, 2010
Last sequence update: February 6, 2007
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families