A1K4S0 (A1K4S0_AZOSB) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase 1 HAMAP MF_01374
EC=3.1.2.6 HAMAP MF_01374

Alternative name(s):
Glyoxalase II 1 HAMAP MF_01374

Gene names

Name:	gloB1 HAMAP MF_01374 EMBL CAL93825.1
Ordered Locus Names:	azo1208

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374 SAAS SAAS017782
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374 SAAS SAAS017782
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374 SAAS SAAS017782
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374 SAAS SAAS017782
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family. HAMAP MF_01374

Ontologies

Keywords
Ligand	Metal-binding HAMAP MF_01374 SAAS SAAS017782 Zinc HAMAP MF_01374 SAAS SAAS017782
Molecular function	Hydrolase HAMAP MF_01374 SAAS SAAS017782 EMBL CAL93825.1
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	57	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	59	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	61	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	62	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	115	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	132	1	Zinc 1 By similarity HAMAP MF_01374
	Metal binding	132	1	Zinc 2 By similarity HAMAP MF_01374
	Metal binding	170	1	Zinc 2 By similarity HAMAP MF_01374

Sequences

	Sequence		Length	Mass (Da)
	A1K4S0 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 6C16034E2CEFFADF	FASTA	258	28,219
	10 20 30 40 50 60 MRPLPLRVRP IPAFQDNYIW LLTRGDEAVV VDPGTAGPVL AVLTAERLRL RAVLITHHHD 70 80 90 100 110 120 DHVGGVAEVL RHAAVPVYGP AGEDIAVLTH RVADGERVAV LDDHPGFEVL ALPGHTAGHI 130 140 150 160 170 180 GYFDGTHLFC GDVLFPCGCG RVFEGTPAQM HAALARLAAL PPATQVYCAH EYSLANARFA 190 200 210 220 230 240 LAAEPGNRLL QGRQRWMEAR RAAGEPTVPS SLRDELDTNP FLRCTEPALR RAAADWRGHP 250 IEDEAEVFAA LRDWKNHF

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM406670 Genomic DNA. Translation: CAL93825.1.
RefSeq	YP_932712.1. NC_008702.1.
3D structure databases
ProteinModelPortal	A1K4S0.
ModBase	Search...
Protein-protein interaction databases
STRING	A1K4S0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4606740.
GenomeReviews	Gene locus azo1208 in contig AM406670_GR.
KEGG	azo:azo1208.
PATRIC	21010756. VBIAzoSp26047_1231.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HBG753931.
OMA	RWILHTH.
PhylomeDB	A1K4S0.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A1K4S0_AZOSB

Accession

Primary (citable) accession number: A1K4S0

Entry history

Integrated into UniProtKB/TrEMBL:	February 6, 2007
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)