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A1K4R7

- SYI_AZOSB

UniProt

A1K4R7 - SYI_AZOSB

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Azoarcus sp. (strain BH72)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (06 Feb 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei567 – 5671Aminoacyl-adenylateUniRule annotation
    Binding sitei611 – 6111ATPUniRule annotation
    Metal bindingi895 – 8951ZincUniRule annotation
    Metal bindingi898 – 8981ZincUniRule annotation
    Metal bindingi915 – 9151ZincUniRule annotation
    Metal bindingi918 – 9181ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:azo1205
    OrganismiAzoarcus sp. (strain BH72)
    Taxonomic identifieri62928 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAzoarcus
    ProteomesiUP000002588: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 932932Isoleucine--tRNA ligasePRO_1000022041Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi62928.azo1205.

    Structurei

    3D structure databases

    ProteinModelPortaliA1K4R7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi58 – 6811"HIGH" regionAdd
    BLAST
    Motifi608 – 6125"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiERLMLHQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A1K4R7-1 [UniParc]FASTAAdd to Basket

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    MADYRKTLNL PDTAFPMRGD LPKREPGWIA AWQQQKLYQK IRKAAAGRPK    50
    FVLHDGPPYA NGNLHLGHAL NKILKDIIVR SKTLAGFDAP YVPGWDCHGL 100
    PIEHKVEVTH GKNLPADKVR ELCRAYAAEQ VEIQKKEFIR LGVLGDWDNP 150
    YLTMNFANEA GEVRALAEMV KAGYVFKGLK PVNWCFDCGS ALAEAEVEYA 200
    DKVSPAVDVG FPCAEPDRLA DAFGLAPLTK PALAVIWTTT PWTIPANQAL 250
    NMHPEFDYAL VDTGERYLVL AHDLVGACLE RYGLNGRIVA TCKGAALDRV 300
    AFRHPFYDRL SPVFLGDYVT LDAGTGVVHS APAYGLEDFQ SCRANGMQSD 350
    DILTPVMGDG RYAPDLPFFG GMNIWKANPE ITAKLREVGC LLAEAKITHS 400
    YMHCWRHKTP LIYRATAQWF VGMDKPVADG STLRERALRG VEATRFFPAW 450
    GQSRLHAMIA NRPDWCISRQ RNWGVPIPFF LHKETGELHP RTVELMEEVA 500
    KRIEQEGIEA WFKLDAAELL GAEAAQYEKI SDTLDVWFDS GTTHWHVLRG 550
    SHNDGHVEGP RADLYLEGSD QHRGWFHSSL LTGCAIDGHP PYNALLTHGF 600
    TVDQQGRKMS KSLGNTILPQ EVSEKMGAEI LRLWVASTDY SGELSISKEI 650
    LDRVVEVYRR VRNTLRFLLA NTADFDIAKD AVPLEQWLDI DRYALAFTRQ 700
    LAQQAEADYA RFEFHRIVQA LQVFCAEDLG AFYLDILKDR LYTTAAGSQA 750
    RRAAQTALWH ITQTLLKLMA PILSFTAEEA WAVLNPGKED SVMLHTFHAL 800
    PAQEGEAGLV ARWETIRAVR AEALKVIEAL RTEGKVGASL QAELELRLTA 850
    DKYTAMQSLG EDLRFVTMTS RATLLEAASA EAEAIVATPS ARTKCERCWH 900
    YTDDVGHNAE HPTLCARCAD NLYGAGETRT HA 932
    Length:932
    Mass (Da):103,961
    Last modified:February 6, 2007 - v1
    Checksum:i93D0B2C8BF654DA5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM406670 Genomic DNA. Translation: CAL93822.1.
    RefSeqiWP_011764938.1. NC_008702.1.
    YP_932709.1. NC_008702.1.

    Genome annotation databases

    EnsemblBacteriaiCAL93822; CAL93822; azo1205.
    GeneIDi4609182.
    KEGGiazo:azo1205.
    PATRICi21010750. VBIAzoSp26047_1228.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM406670 Genomic DNA. Translation: CAL93822.1 .
    RefSeqi WP_011764938.1. NC_008702.1.
    YP_932709.1. NC_008702.1.

    3D structure databases

    ProteinModelPortali A1K4R7.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 62928.azo1205.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAL93822 ; CAL93822 ; azo1205 .
    GeneIDi 4609182.
    KEGGi azo:azo1205.
    PATRICi 21010750. VBIAzoSp26047_1228.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi ERLMLHQ.
    OrthoDBi EOG644ZM1.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: BH72.

    Entry informationi

    Entry nameiSYI_AZOSB
    AccessioniPrimary (citable) accession number: A1K4R7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3