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A1K4R7

- SYI_AZOSB

UniProt

A1K4R7 - SYI_AZOSB

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Protein

Isoleucine--tRNA ligase

Gene
ileS, azo1205
Organism
Azoarcus sp. (strain BH72)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei567 – 5671Aminoacyl-adenylate By similarity
Binding sitei611 – 6111ATP By similarity
Metal bindingi895 – 8951Zinc By similarity
Metal bindingi898 – 8981Zinc By similarity
Metal bindingi915 – 9151Zinc By similarity
Metal bindingi918 – 9181Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:azo1205
OrganismiAzoarcus sp. (strain BH72)
Taxonomic identifieri62928 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAzoarcus
ProteomesiUP000002588: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 932932Isoleucine--tRNA ligaseUniRule annotationPRO_1000022041Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi62928.azo1205.

Structurei

3D structure databases

ProteinModelPortaliA1K4R7.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi608 – 6125"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1K4R7-1 [UniParc]FASTAAdd to Basket

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MADYRKTLNL PDTAFPMRGD LPKREPGWIA AWQQQKLYQK IRKAAAGRPK    50
FVLHDGPPYA NGNLHLGHAL NKILKDIIVR SKTLAGFDAP YVPGWDCHGL 100
PIEHKVEVTH GKNLPADKVR ELCRAYAAEQ VEIQKKEFIR LGVLGDWDNP 150
YLTMNFANEA GEVRALAEMV KAGYVFKGLK PVNWCFDCGS ALAEAEVEYA 200
DKVSPAVDVG FPCAEPDRLA DAFGLAPLTK PALAVIWTTT PWTIPANQAL 250
NMHPEFDYAL VDTGERYLVL AHDLVGACLE RYGLNGRIVA TCKGAALDRV 300
AFRHPFYDRL SPVFLGDYVT LDAGTGVVHS APAYGLEDFQ SCRANGMQSD 350
DILTPVMGDG RYAPDLPFFG GMNIWKANPE ITAKLREVGC LLAEAKITHS 400
YMHCWRHKTP LIYRATAQWF VGMDKPVADG STLRERALRG VEATRFFPAW 450
GQSRLHAMIA NRPDWCISRQ RNWGVPIPFF LHKETGELHP RTVELMEEVA 500
KRIEQEGIEA WFKLDAAELL GAEAAQYEKI SDTLDVWFDS GTTHWHVLRG 550
SHNDGHVEGP RADLYLEGSD QHRGWFHSSL LTGCAIDGHP PYNALLTHGF 600
TVDQQGRKMS KSLGNTILPQ EVSEKMGAEI LRLWVASTDY SGELSISKEI 650
LDRVVEVYRR VRNTLRFLLA NTADFDIAKD AVPLEQWLDI DRYALAFTRQ 700
LAQQAEADYA RFEFHRIVQA LQVFCAEDLG AFYLDILKDR LYTTAAGSQA 750
RRAAQTALWH ITQTLLKLMA PILSFTAEEA WAVLNPGKED SVMLHTFHAL 800
PAQEGEAGLV ARWETIRAVR AEALKVIEAL RTEGKVGASL QAELELRLTA 850
DKYTAMQSLG EDLRFVTMTS RATLLEAASA EAEAIVATPS ARTKCERCWH 900
YTDDVGHNAE HPTLCARCAD NLYGAGETRT HA 932
Length:932
Mass (Da):103,961
Last modified:February 6, 2007 - v1
Checksum:i93D0B2C8BF654DA5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM406670 Genomic DNA. Translation: CAL93822.1.
RefSeqiWP_011764938.1. NC_008702.1.
YP_932709.1. NC_008702.1.

Genome annotation databases

EnsemblBacteriaiCAL93822; CAL93822; azo1205.
GeneIDi4609182.
KEGGiazo:azo1205.
PATRICi21010750. VBIAzoSp26047_1228.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM406670 Genomic DNA. Translation: CAL93822.1 .
RefSeqi WP_011764938.1. NC_008702.1.
YP_932709.1. NC_008702.1.

3D structure databases

ProteinModelPortali A1K4R7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 62928.azo1205.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL93822 ; CAL93822 ; azo1205 .
GeneIDi 4609182.
KEGGi azo:azo1205.
PATRICi 21010750. VBIAzoSp26047_1228.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BH72.

Entry informationi

Entry nameiSYI_AZOSB
AccessioniPrimary (citable) accession number: A1K4R7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: September 3, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi