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A1K4R7

- SYI_AZOSB

UniProt

A1K4R7 - SYI_AZOSB

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Azoarcus sp. (strain BH72)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei567 – 5671Aminoacyl-adenylateUniRule annotation
Binding sitei611 – 6111ATPUniRule annotation
Metal bindingi895 – 8951ZincUniRule annotation
Metal bindingi898 – 8981ZincUniRule annotation
Metal bindingi915 – 9151ZincUniRule annotation
Metal bindingi918 – 9181ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:azo1205
OrganismiAzoarcus sp. (strain BH72)
Taxonomic identifieri62928 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaRhodocyclalesRhodocyclaceaeAzoarcus
ProteomesiUP000002588: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 932932Isoleucine--tRNA ligasePRO_1000022041Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi62928.azo1205.

Structurei

3D structure databases

ProteinModelPortaliA1K4R7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi608 – 6125"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1K4R7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADYRKTLNL PDTAFPMRGD LPKREPGWIA AWQQQKLYQK IRKAAAGRPK
60 70 80 90 100
FVLHDGPPYA NGNLHLGHAL NKILKDIIVR SKTLAGFDAP YVPGWDCHGL
110 120 130 140 150
PIEHKVEVTH GKNLPADKVR ELCRAYAAEQ VEIQKKEFIR LGVLGDWDNP
160 170 180 190 200
YLTMNFANEA GEVRALAEMV KAGYVFKGLK PVNWCFDCGS ALAEAEVEYA
210 220 230 240 250
DKVSPAVDVG FPCAEPDRLA DAFGLAPLTK PALAVIWTTT PWTIPANQAL
260 270 280 290 300
NMHPEFDYAL VDTGERYLVL AHDLVGACLE RYGLNGRIVA TCKGAALDRV
310 320 330 340 350
AFRHPFYDRL SPVFLGDYVT LDAGTGVVHS APAYGLEDFQ SCRANGMQSD
360 370 380 390 400
DILTPVMGDG RYAPDLPFFG GMNIWKANPE ITAKLREVGC LLAEAKITHS
410 420 430 440 450
YMHCWRHKTP LIYRATAQWF VGMDKPVADG STLRERALRG VEATRFFPAW
460 470 480 490 500
GQSRLHAMIA NRPDWCISRQ RNWGVPIPFF LHKETGELHP RTVELMEEVA
510 520 530 540 550
KRIEQEGIEA WFKLDAAELL GAEAAQYEKI SDTLDVWFDS GTTHWHVLRG
560 570 580 590 600
SHNDGHVEGP RADLYLEGSD QHRGWFHSSL LTGCAIDGHP PYNALLTHGF
610 620 630 640 650
TVDQQGRKMS KSLGNTILPQ EVSEKMGAEI LRLWVASTDY SGELSISKEI
660 670 680 690 700
LDRVVEVYRR VRNTLRFLLA NTADFDIAKD AVPLEQWLDI DRYALAFTRQ
710 720 730 740 750
LAQQAEADYA RFEFHRIVQA LQVFCAEDLG AFYLDILKDR LYTTAAGSQA
760 770 780 790 800
RRAAQTALWH ITQTLLKLMA PILSFTAEEA WAVLNPGKED SVMLHTFHAL
810 820 830 840 850
PAQEGEAGLV ARWETIRAVR AEALKVIEAL RTEGKVGASL QAELELRLTA
860 870 880 890 900
DKYTAMQSLG EDLRFVTMTS RATLLEAASA EAEAIVATPS ARTKCERCWH
910 920 930
YTDDVGHNAE HPTLCARCAD NLYGAGETRT HA
Length:932
Mass (Da):103,961
Last modified:February 6, 2007 - v1
Checksum:i93D0B2C8BF654DA5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM406670 Genomic DNA. Translation: CAL93822.1.
RefSeqiWP_011764938.1. NC_008702.1.
YP_932709.1. NC_008702.1.

Genome annotation databases

EnsemblBacteriaiCAL93822; CAL93822; azo1205.
GeneIDi4609182.
KEGGiazo:azo1205.
PATRICi21010750. VBIAzoSp26047_1228.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM406670 Genomic DNA. Translation: CAL93822.1 .
RefSeqi WP_011764938.1. NC_008702.1.
YP_932709.1. NC_008702.1.

3D structure databases

ProteinModelPortali A1K4R7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 62928.azo1205.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL93822 ; CAL93822 ; azo1205 .
GeneIDi 4609182.
KEGGi azo:azo1205.
PATRICi 21010750. VBIAzoSp26047_1228.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: BH72.

Entry informationi

Entry nameiSYI_AZOSB
AccessioniPrimary (citable) accession number: A1K4R7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: October 29, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3