A1K4R0 (DXS_AZOSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	azo1198

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	619 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 619	619	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_1000019007

Sequences

	Sequence		Length	Mass (Da)
	A1K4R0 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 5100A8DD1EFFE81F	FASTA	619	66,358
	10 20 30 40 50 60 MSPYPHLERI GSPADLRATE RRELAQVASE LRAFLIESVS KTGGHLSSNL GTVELTIALH 70 80 90 100 110 120 YVFNTPDDRI VWDVGHQTYG HKILTGRREA MSGLRHWGGI SGFPRRCESE YDTFGTAHSS 130 140 150 160 170 180 TSISAALGMA VAARDRGEDR RAIAVIGDGA MSAGMAFEAL NNAGDMDANL LVILNDNEMS 190 200 210 220 230 240 ISPPVGALTK ILARLMSGST YNAARRVGEK VLGTVPPMAE LARKVEEYAK GMIAPGTLFE 250 260 270 280 290 300 EFGFHYYGPI DGHDLDALIP TLQNIRKLKG PQFLHVITKK GQGYKLAEAD PILYHGVSKF 310 320 330 340 350 360 DHTAGIQTGK SGGKLTYTQV FSDWLCDIAA ADPRIVGITP AMREGSGLVE FAQRFPDRYY 370 380 390 400 410 420 DVGIAEQHAL TFAAGLACEG LKPVVAIYST FLQRAYDQLI HDIALQNLPV VLAIDRGGLV 430 440 450 460 470 480 GADGATHHGA FDLSFLACVP NLVVMAPADE NECRQMLYTA VCHDGPTAVR YPRGGGSGVV 490 500 510 520 530 540 PLEPMTALPI GKGEIRRHGT RIAVLAFGSM LGVALEVGEA LDASVANMRF VKPLDEALIA 550 560 570 580 590 600 ELAANHALLV TVEENAVIGG AGSEVARFVD TLPQRPRVLR LGLPDRFIDH GDQAQLLASV 610 GLDKTGILAA IEAVYPHNS

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BH72.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM406670 Genomic DNA. Translation: CAL93815.1.
RefSeq	YP_932702.1. NC_008702.1.
3D structure databases
ProteinModelPortal	A1K4R0.
SMR	A1K4R0. Positions 1-614.
ModBase	Search...
Protein-protein interaction databases
STRING	A1K4R0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4606738.
GenomeReviews	Gene locus azo1198 in contig AM406670_GR.
KEGG	azo:azo1198.
PATRIC	21010736. VBIAzoSp26047_1221.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1154.
HOGENOM	HBG571647.
OMA	DPILYHG.
PhylomeDB	A1K4R0.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	ASP62928:AZO1198-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR001017. DH_E1. IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF00676. E1_dh. 1 hit. PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_AZOSB

Accession

Primary (citable) accession number: A1K4R0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program