Reviewed, UniProtKB/Swiss-Prot A1K4K7 (ACEK_AZOSB)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	azo1145

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 589	589	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000288281
Regions
	Nucleotide binding	322 – 328	7	ATP By similarity
Sites
	Active site	378	1	By similarity
	Binding site	343	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1K4K7-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: E52F3B1A6F4AE05E	FASTA	589	68,168
	10 20 30 40 50 60 MDAQVGDNPV AQAIAHALVE GFNKHYRIFR GTSRRAKEYF EAGDWRAQLD AVRDRVQFYD 70 80 90 100 110 120 DRVDETVRRL LEEFDADSLD DATWQQVKLH FIGALINHKQ PELAETFFNS VGCKILHRTY 130 140 150 160 170 180 FNNDYIFARP AISTEYIESY PPVYSSYYPQ DGGLRATIRR IIEDFDWQRP FEDLDRDIDC 190 200 210 220 230 240 IMRAALAHLG EWPAMEVNCQ LQVLYSAFYR NKTAYIIGKV INGWQDYPFT LAVRHGASGR 250 260 270 280 290 300 LVIDTILLDP WRISVLFSLS RAYFMVDMEV PSGYVQFLRS IMPNKPRSEL YTMLGLGKQG 310 320 330 340 350 360 KTMFFRDLVA HLRHSNDQFI IAPGIRGLVM LVFTLPSYPY VFKIIKDVFG SSKNMDRATV 370 380 390 400 410 420 KRKYLMVKQV DRVGRMADTL EFSYAALPLS RFHPELLEEL RTLAPSAFEI DGDALVLKHF 430 440 450 460 470 480 YIERRMTPLN IHLEKASDGE VEAAVHEYGN AIRELAIANI FPGDMLWKNF GVTRYGRVVF 490 500 510 520 530 540 YDYDEIEYMT SMNFRRIPPA PHEDMELSGE PWYSAGPMDV FPEEFATFLL GSPRVRKAFM 550 560 570 580 KYHRDLLEPA FWQAAQQGVR DGHVEDFFPY PAELRFSVMF PATPVAAQD

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM406670 Genomic DNA. Translation: CAL93762.1.
RefSeq	YP_932649.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1K4K7.
Genome annotation databases
GeneID	4609886.
GenomeReviews	Gene locus azo1145 in contig AM406670_GR.
KEGG	azo:azo1145.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	FYRDFLH.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_AZOSB

Accession

Primary (citable) accession number: A1K4K7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)