A1K4K1 (DUT_AZOSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	azo1139

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP-Rule MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP-Rule MF_00116
Cofactor	Magnesium By similarity. HAMAP-Rule MF_00116
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP-Rule MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dUMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	dUTP diphosphatase activity Inferred from electronic annotation. Source: UniProtKB-HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 149	149	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP-Rule MF_00116		PRO_1000015443
Regions
	Region	68 – 70	3	Substrate binding By similarity
	Region	85 – 87	3	Substrate binding By similarity
Sites
	Binding site	81	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1K4K1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: B7ED0FA1BEF6E66F	FASTA	149	15,758
	10 20 30 40 50 60 MHRIDVKLLD PRLKTHPPAY ASAGAAGLDL RACLDAPVLL HPGETTLVPS GLAIHLADPG 70 80 90 100 110 120 LAAMVLPRSG LGHKHGIVLG NLVGLIDSDY QGQVFVSVWN RGRDVFTIQP MERIAQLVVV 130 140 PVLQVGFNVV DDFAASERGE GGFGSTGKH

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BH72.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM406670 Genomic DNA. Translation: CAL93756.1.
RefSeq	YP_932643.1. NC_008702.1.
3D structure databases
ProteinModelPortal	A1K4K1.
SMR	A1K4K1. Positions 1-136.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	62928.azo1139.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAL93756; CAL93756; azo1139.
GeneID	4608619.
KEGG	azo:azo1139.
PATRIC	21010612. VBIAzoSp26047_1160.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0756.
HOGENOM	HOG000028968.
KO	K01520.
OMA	EPMERIA.
OrthoDB	EOG689HXK.
Enzyme and pathway databases
UniPathway	UPA00610; UER00666.
Family and domain databases
Gene3D	2.70.40.10. 1 hit.
HAMAP	MF_00116. dUTPase_bact.
InterPro	IPR029054. dUTPase-like. IPR008180. dUTPase/dCTP_deaminase. IPR008181. dUTPase_1. [Graphical view]
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
SUPFAM	SSF51283. SSF51283. 1 hit.
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_AZOSB

Accession

Primary (citable) accession number: A1K4K1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	June 11, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program