A1K4E5 (SYFA_AZOSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	azo1083

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 343	343	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_1000006798
Sites
	Metal binding	264	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1K4E5 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 076AB3B1D6E7EF91	FASTA	343	38,161
	10 20 30 40 50 60 MDNLDQLVQQ AQADFAGVSD SAQLEQAKAR YLGKSGALTE QLKGLGKLPP EEKREAGAAI 70 80 90 100 110 120 NRAKTAIEAA LEARRNALRE AALLAQLAAE ALDVTLPGRG AQGGGLHPVS RTLERIEMLF 130 140 150 160 170 180 RSIGFIVADG PEIETDWHNF TALNTPENHP ARSMHDTFYL EGRDDVLLRT HTSPVQIRTM 190 200 210 220 230 240 LAHVKRHAEA AAMPEIRVII PGRVYRVDSD ATHSPMFHQV EGLWVGEKVS FADLKGVIAD 250 260 270 280 290 300 FLRKFFETED LQVRFRPSFF PFTEPSAEID VAFMSGPLKG RWLEIAGCGM VHPNVLRFGG 310 320 330 340 VDPERYTGFA FGMGPDRLTM LRYGVNDLRL FFEGDLRFLS QFR

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BH72.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM406670 Genomic DNA. Translation: CAL93700.1.
RefSeq	YP_932587.1. NC_008702.1.
3D structure databases
ProteinModelPortal	A1K4E5.
ModBase	Search...
Protein-protein interaction databases
STRING	A1K4E5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4609779.
GenomeReviews	Gene locus azo1083 in contig AM406670_GR.
KEGG	azo:azo1083.
PATRIC	21010494. VBIAzoSp26047_1103.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0016.
HOGENOM	HBG284353.
OMA	FRASYFP.
PhylomeDB	A1K4E5.
ProtClustDB	PRK00488.
Enzyme and pathway databases
BioCyc	ASP62928:AZO1083-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_AZOSB

Accession

Primary (citable) accession number: A1K4E5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program