Reviewed, UniProtKB/Swiss-Prot A1K4E1 (SYT_AZOSB)

Names and origin

Protein names

Recommended name:
    Threonyl-tRNA synthetase
    EC=6.1.1.3
Alternative name(s):
    Threonine--tRNA ligase
      Short name=ThrRS

Gene names

Name:	thrS
Ordered Locus Names:	azo1079

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	638 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr). HAMAP MF_00184
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00184
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	threonyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP threonine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 638	638	Threonyl-tRNA synthetase HAMAP MF_00184		PRO_1000020340
Regions
	Region	242 – 533	292	Catalytic HAMAP MF_00184
Sites
	Metal binding	333	1	Zinc; catalytic By similarity
	Metal binding	384	1	Zinc; catalytic By similarity
	Metal binding	510	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1K4E1-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 211CC29537E1982F	FASTA	638	72,143
	10 20 30 40 50 60 MPNIKLPDGS VRSFDHPVTV AEVAASIGSG LAKAALAGRV DGKAVDLSYC IERDSELAIL 70 80 90 100 110 120 TDKSAEGVDV IRHSTAHLLA HAVKELFPEA QVTIGPVIEN GFYYDFSYKR PFTPEDLEAI 130 140 150 160 170 180 EKRMAELVKR ELPVEREVWP RDKAVAFFKS IGEHYKAEII ASIPANEDVS LYRQGEFVDL 190 200 210 220 230 240 CRGPHVPSTG RLKVFKLTKL AGAYWRGDAK NEMLQRVYGT AWAKKEDLES YLHMLEEAEK 250 260 270 280 290 300 RDHRKLGRQL DLFHIQEEAP GMVFWHAKGW TLWQQVEQYL RGTISRHGYQ EVRTPQIVDR 310 320 330 340 350 360 SLWEKSGHWG MYSDLMFTTQ SEKHDYAVKP MNCPCHIQIF NQGLKSYRDL PLRMAEFGSC 370 380 390 400 410 420 HRNEPSGSLH GIMRVRNFVQ DDAHIFCTDE QVQEEAAAFI ALLQKVYADF GFRDIQIKLS 430 440 450 460 470 480 TRPEKRVGAD EQWDAAEAAL AAALKAQGLE YELQPGEGAF YGPKIEFSLK DCLNRVWQCG 490 500 510 520 530 540 TLQLDFNLPV RLGAEYVAED NTKKFPVMLH RAIVGSLERF IGILIEHYAG AMPLWLAPVH 550 560 570 580 590 600 AVVMNISEGQ ADYATEVVNR LREAGFRVEA DLRNEKINYK IREHSVHKLP YQLVVGEKEK 610 620 630 AAGVVAVRVR GGQDLGQLSL DKLIERWNGE LASRSGPV

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM406670 Genomic DNA. Translation: CAL93696.1.
RefSeq	YP_932583.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1K4E1.
Genome annotation databases
GeneID	4610025.
GenomeReviews	Gene locus azo1079 in contig AM406670_GR.
KEGG	azo:azo1079.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	QDEAPGM.
Family and domain databases
HAMAP	MF_00184. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR012675. b-grasp_ferredoxin-like. IPR004095. TGS. IPR002320. Thr-tRNA-synth_IIa. IPR018158. Thr-tRNA-synth_IIa_cons-reg. IPR012947. tRNA_SAD. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF02824. TGS. 1 hit. PF00587. tRNA-synt_2b. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR01047. TRNASYNTHTHR.
TIGRFAMs	TIGR00418. thrS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYT_AZOSB

Accession

Primary (citable) accession number: A1K4E1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	November 3, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)