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Reviewed, UniProtKB/Swiss-Prot A1K3W9 (TGT_AZOSB)

Last modified June 16, 2009. Version 15. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Queuine tRNA-ribosyltransferase
    EC=2.4.2.29
Alternative name(s):
    tRNA-guanine transglycosylase
    Guanine insertion enzyme
Gene names
Name: tgt
Ordered Locus Names: azo0907
OrganismAzoarcus sp. (strain BH72) [Complete proteome] [HAMAP]
Taxonomic identifier62928 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

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Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity.

Catalytic activity

[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168

[tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168

Cofactor

Binds 1 zinc ion per subunit By similarity.

Pathway

tRNA modification; queuosine-tRNA biosynthesis. HAMAP MF_00168

Sequence similarities

Belongs to the queuine tRNA-ribosyltransferase family.

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Ontologies

Keywords
   Biological processQueuosine biosynthesis
tRNA processing
   LigandMetal-binding
Zinc
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processqueuosine biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Molecular functionqueuine tRNA-ribosyltransferase activity

Inferred from electronic annotation. Source: HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 370370Queuine tRNA-ribosyltransferase HAMAP MF_00168
PRO_1000016762

Sites

Active site891Nucleophile By similarity
Metal binding3021Zinc By similarity
Metal binding3041Zinc By similarity
Metal binding3071Zinc By similarity
Metal binding3331Zinc By similarity
Binding site901Substrate By similarity

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Sequences

Sequence LengthMass (Da)Tools
A1K3W9-1 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: C2BD8C0FCB7EE874

FASTA37040,941
        10         20         30         40         50         60 
MQFELLSTSA GARRGRLTLA HGAVETPVFM PVGTYGTVKA MTPAMLSDVG AQICLGNTFH 

        70         80         90        100        110        120 
LWLRPGLDIV GAHGGLHRFM GWDKPILTDS GGFQVFSLGA LRKISEEGVK FASPIDGARL 

       130        140        150        160        170        180 
FLTPEISMQI QTVLNSDVVM IFDECTPYPA TRDEAAKSMR LSRRWARRSR DEFDRLENAN 

       190        200        210        220        230        240 
ALFGIVQGGM YEDLRDESLG ALQDIGFHGF AIGGLSVGEP KDDMARILAH TAPRLPADKP 

       250        260        270        280        290        300 
RYLMGVGTPE DIVDGIANGI DMFDCVMPTR NARNGWLFTR YGDLKIKNAV HKADTRPLDP 

       310        320        330        340        350        360 
SCSCYTCRNF SRSYLHHLHR AGEILGSMLN TVHNLHYYQT LTAELRDAIA ADRFADYVTR 

       370 
FRSERATGAH

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References

[1]"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72."
Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. expand/collapse author list , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A.
Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

AM406670 Genomic DNA. Translation: CAL93524.1.
RefSeqYP_932411.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID4606629.
GenomeReviewsGene locus azo0907 in contig AM406670_GR.
KEGGazo:azo0907.

Organism-specific databases

CMRSearch...

Phylogenomic databases

OMAA1K3W9. VLNSDIV.

Family and domain databases

HAMAPMF_00168.
[Tree]
InterProIPR004803. QtRNA_ribo_trans.
IPR002616. tRNA_ribo_trans.
[Graphical view]
Gene3DG3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
PANTHERPTHR11962. tRNA_ribo_trans. 1 hit.
PfamPF01702. TGT. 1 hit.
[Graphical view]
TIGRFAMsTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameTGT_AZOSB
AccessionPrimary (citable) accession number: A1K3W9
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: June 16, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents