A1K3W9 (TGT_AZOSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Queuine tRNA-ribosyltransferase
EC=2.4.2.29

Alternative name(s):
Guanine insertion enzyme
tRNA-guanine transglycosylase

Gene names

Name:	tgt
Ordered Locus Names:	azo0907

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) By similarity. HAMAP MF_00168
Catalytic activity	[tRNA]-guanine + queuine = [tRNA]-queuine + guanine. HAMAP MF_00168 [tRNA]-guanine + 7-aminomethyl-7-carbaguanine = [tRNA]-7-aminomethyl-7-carbaguanine + guanine. HAMAP MF_00168
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00168
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00168
Sequence similarities	Belongs to the queuine tRNA-ribosyltransferase family.

Ontologies

Keywords
Biological process	Queuosine biosynthesis tRNA processing
Ligand	Metal-binding Zinc
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW queuine tRNA-ribosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	Queuine tRNA-ribosyltransferase HAMAP MF_00168		PRO_1000016762
Sites
	Active site	89	1	Nucleophile By similarity
	Metal binding	302	1	Zinc By similarity
	Metal binding	304	1	Zinc By similarity
	Metal binding	307	1	Zinc By similarity
	Metal binding	333	1	Zinc By similarity
	Binding site	90	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1K3W9 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: C2BD8C0FCB7EE874	FASTA	370	40,941
	10 20 30 40 50 60 MQFELLSTSA GARRGRLTLA HGAVETPVFM PVGTYGTVKA MTPAMLSDVG AQICLGNTFH 70 80 90 100 110 120 LWLRPGLDIV GAHGGLHRFM GWDKPILTDS GGFQVFSLGA LRKISEEGVK FASPIDGARL 130 140 150 160 170 180 FLTPEISMQI QTVLNSDVVM IFDECTPYPA TRDEAAKSMR LSRRWARRSR DEFDRLENAN 190 200 210 220 230 240 ALFGIVQGGM YEDLRDESLG ALQDIGFHGF AIGGLSVGEP KDDMARILAH TAPRLPADKP 250 260 270 280 290 300 RYLMGVGTPE DIVDGIANGI DMFDCVMPTR NARNGWLFTR YGDLKIKNAV HKADTRPLDP 310 320 330 340 350 360 SCSCYTCRNF SRSYLHHLHR AGEILGSMLN TVHNLHYYQT LTAELRDAIA ADRFADYVTR 370 FRSERATGAH

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BH72.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM406670 Genomic DNA. Translation: CAL93524.1.
RefSeq	YP_932411.1. NC_008702.1.
3D structure databases
ProteinModelPortal	A1K3W9.
SMR	A1K3W9. Positions 1-363.
ModBase	Search...
Protein-protein interaction databases
STRING	A1K3W9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4606629.
GenomeReviews	Gene locus azo0907 in contig AM406670_GR.
KEGG	azo:azo0907.
PATRIC	21010110. VBIAzoSp26047_0923.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0343.
HOGENOM	HBG629929.
OMA	FMPVGTV.
PhylomeDB	A1K3W9.
ProtClustDB	PRK00112.
Enzyme and pathway databases
BioCyc	ASP62928:AZO0907-MONOMER.
Family and domain databases
HAMAP	MF_00168. Q_tRNA_Tgt. [Tree]
InterPro	IPR004803. Queuine_tRNA-ribosylTrfase. IPR002616. tRNA_ribo_trans. [Graphical view]
Gene3D	G3DSA:3.20.20.105. tRNA_ribo_trans. 1 hit.
KO	K00773.
PANTHER	PTHR11962. tRNA_ribo_trans. 1 hit.
Pfam	PF01702. TGT. 1 hit. [Graphical view]
SUPFAM	SSF51713. tRNA_ribo_trans. 1 hit.
TIGRFAMs	TIGR00430. Q_tRNA_tgt. 1 hit. TIGR00449. Tgt_general. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TGT_AZOSB

Accession

Primary (citable) accession number: A1K3W9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program