A1K3U7 (MURC_AZOSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramate--L-alanine ligase
EC=6.3.2.8

Alternative name(s):
UDP-N-acetylmuramoyl-L-alanine synthetase

Gene names

Name:	murC
Ordered Locus Names:	azo0885

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00046
Catalytic activity	ATP + UDP-N-acetylmuramate + L-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanine. HAMAP MF_00046
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00046
Subcellular location	Cytoplasm By similarity HAMAP MF_00046.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramate-L-alanine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	UDP-N-acetylmuramate--L-alanine ligase HAMAP MF_00046		PRO_1000004308
Regions
	Nucleotide binding	112 – 118	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A1K3U7 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: A1D11D20E0968108	FASTA	467	49,290
	10 20 30 40 50 60 MKHKVKRIHF VGIGGAGMSG IAEVLVNLGY SVSGSDLGDS AATRRLKAMG AKVVLGHDAA 70 80 90 100 110 120 NVEAADALVV STAVKNDNPE VIAARARHIP IVPRAQMLAE LMRLKSGIAI AGTHGKTTTT 130 140 150 160 170 180 SLVASILAEG AMDPTFVIGG RLNAAGANAR LGKGDFLVAE ADESDASFLM LSPVISVVTN 190 200 210 220 230 240 IDADHMDTYG HDFARLKQAF VDFLQRLPFY GVAVLCEDDP HVRSIMPLVS KQVVRYGLSE 250 260 270 280 290 300 TANIRAENIR AEGGRMIFDV LRVNGSTTRL ADVVLNLPGL HNVRNALAAI AVATEVQVPD 310 320 330 340 350 360 EAIVKALAEF SGVGRRFQRY GEVAAPSGGT FTLIDDYGHH PVEMEATLAA ARGAFPGRRL 370 380 390 400 410 420 VLAFQPHRYT RTRDCFEDFV KVLSTVDALL LAEVYAAGEA PIVAADGRAL SRALRVAGKV 430 440 450 460 EPVFVEDIGG MPQAVLEAVR DGDVVITMGA GSIGAVPGKL ASNEEQA

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BH72.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM406670 Genomic DNA. Translation: CAL93502.1.
RefSeq	YP_932389.1. NC_008702.1.
3D structure databases
ProteinModelPortal	A1K3U7.
SMR	A1K3U7. Positions 4-462.
ModBase	Search...
Protein-protein interaction databases
STRING	A1K3U7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4609293.
GenomeReviews	Gene locus azo0885 in contig AM406670_GR.
KEGG	azo:azo0885.
PATRIC	21010066. VBIAzoSp26047_0901.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0773.
HOGENOM	HBG476594.
OMA	YQPHRYS.
PhylomeDB	A1K3U7.
ProtClustDB	PRK00421.
Enzyme and pathway databases
BioCyc	ASP62928:AZO0885-MONOMER.
Family and domain databases
HAMAP	MF_00046. MurC. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR016040. NAD(P)-bd_dom. IPR005758. UDP-N-AcMur_Ala_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01924.
PANTHER	PTHR23135:SF5. PTHR23135:SF5. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01082. MurC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURC_AZOSB

Accession

Primary (citable) accession number: A1K3U7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program