Reviewed, UniProtKB/Swiss-Prot A1K3U3 (MRAY_AZOSB)

Names and origin

Protein names

Recommended name:
    Phospho-N-acetylmuramoyl-pentapeptide-transferase
    EC=2.7.8.13
Alternative name(s):
    UDP-MurNAc-pentapeptide phosphotransferase

Gene names

Name:	mraY
Ordered Locus Names:	azo0881

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan By similarity. HAMAP MF_00038
Catalytic activity	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala) + undecaprenyl phosphate = UMP + Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol. HAMAP MF_00038
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00038
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP MF_00038.
Sequence similarities	Belongs to the glycosyltransferase 4 family. MraY subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	phospho-N-acetylmuramoyl-pentapeptide-transferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Phospho-N-acetylmuramoyl-pentapeptide-transferase HAMAP MF_00038		PRO_1000002936
Regions
	Transmembrane	18 – 38	21	Potential
	Transmembrane	73 – 93	21	Potential
	Transmembrane	97 – 117	21	Potential
	Transmembrane	134 – 154	21	Potential
	Transmembrane	160 – 180	21	Potential
	Transmembrane	200 – 220	21	Potential
	Transmembrane	237 – 257	21	Potential
	Transmembrane	264 – 284	21	Potential
	Transmembrane	289 – 309	21	Potential
	Transmembrane	339 – 359	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	A1K3U3-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 09D683B38BA056C7	FASTA	362	38,801
	10 20 30 40 50 60 MLLELALWLG QDIRGFNVFG YITLRTVLAA LTALAISLTA GPGVIRWLAA KKIGQAVRDD 70 80 90 100 110 120 GPKSHLTKAG TPTMGGALIL IAIGITILLW GDLRNAYVWV TLLVTLGFGA VGWVDDWRKV 130 140 150 160 170 180 VHRDPKGLAS RWKYFWTSAI ALGASIFLGL SATTPAETEL IVPFFKAVAY PLGVYGFIAL 190 200 210 220 230 240 TYFVINGTSH AVNLTDGLDG LAIMPTVMVA GALAIFAYVA GHAGFSKYLG VPYIAGAGEL 250 260 270 280 290 300 AVFCGAICGA GLGFLWFNAY PAEVFMGDVG ALALGAALGT IAVVVRQEIV LFIMGGLFVA 310 320 330 340 350 360 ETLSVMVQVL YFKASGGKRI FRMAPLHHHY ELSGWKETQV VVRFWIITLM LVLFGLSTLK LR

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM406670 Genomic DNA. Translation: CAL93498.1.
RefSeq	YP_932385.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1K3U3.
Genome annotation databases
GeneID	4609292.
GenomeReviews	Gene locus azo0881 in contig AM406670_GR.
KEGG	azo:azo0881.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0472.
HOGENOM	HBG708263.
OMA	HHHFEQK.
PhylomeDB	A1K3U3.
Family and domain databases
HAMAP	MF_00038. MraY. [Tree]
InterPro	IPR000715. Glycosyl_transferase_4. IPR018481. Glycosyl_Trfase_4_cons-reg. IPR003524. PNAcMuramoyl-5peptid_Trfase. IPR018480. PNAcMuramoyl-5peptid_Trfase_CS. [Graphical view]
PANTHER	PTHR22926. Glyco_trans_4. 1 hit. PTHR22926:SF3. PNAcPpept_trans. 1 hit.
Pfam	PF00953. Glycos_transf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00445. mraY. 1 hit.
PROSITE	PS01347. MRAY_1. 1 hit. PS01348. MRAY_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MRAY_AZOSB

Accession

Primary (citable) accession number: A1K3U3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	February 9, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)