Dihydroorotase - Azoarcus sp. (strain BH72)

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Reviewed, UniProtKB/Swiss-Prot A1K3T5 (PYRC_AZOSB)

Last modified June 16, 2009. Version 20. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	azo0873

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H₂O = N-carbamoyl-L-aspartate. HAMAP MF_00219
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6. HAMAP MF_00219
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

344

Dihydroorotase HAMAP MF_00219

PRO_0000325569

Sites

Metal binding

1

Zinc 1 By similarity

Metal binding

1

Zinc 1 By similarity

Metal binding

1

Zinc 1; via carbamate group By similarity

Metal binding

1

Zinc 2; via carbamate group By similarity

Metal binding

1

Zinc 2 By similarity

Metal binding

1

Zinc 2 By similarity

Metal binding

1

Zinc 1 By similarity

Amino acid modifications

Modified residue

1

N6-carboxylysine By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

A1K3T5-1 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: D7B70D3E29E5EDFA
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344

37,002

        10         20         30         40         50         60 
MQSITFTRPD DWHLHVRDGA ALAAVVPHTA ERFGRALIMP NLRPPVTTTA QALAYRDRIR 

        70         80         90        100        110        120 
AAVPAGLAFE PLMSLYLTDN TAPDEIERAR ASGAVIAAKL YPAGATTNSD AGVTAIDKIY 

       130        140        150        160        170        180 
PVLERMEACG LVLCVHGEVT GGEVDVFDRE RVFMEKILSP LVRRFPALKV VFEHITTAEA 

       190        200        210        220        230        240 
AQFVRAAGAN VAATVTAHHL LLNRNAIFAG GIRPHHYCLP VLKRETHRVA LVEAVTSGNP 

       250        260        270        280        290        300 
RFFLGTDSAP HARSTKEAAC GCAGCYTAHA GIELYAEVFD AAGALERLEA FASLNGPAFY 

       310        320        330        340 
GLAPNADKIT LVRESWSVPA GFPYLDDDPL VPLRAGESVG WRLA

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72."
Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S.

expand/collapse author list

Goesmann A.
Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
	AM406670 Genomic DNA. Translation: CAL93490.1.
RefSeq	YP_932377.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4606619.
GenomeReviews	Gene locus azo0873 in contig AM406670_GR.
KEGG	azo:azo0873.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A1K3T5. IMPNLVP.
Family and domain databases
HAMAP	MF_00219. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. pyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_AZOSB

Accession

Primary (citable) accession number: A1K3T5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	February 6, 2007
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents