Reviewed, UniProtKB/Swiss-Prot A1K3T5 (PYRC_AZOSB)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	azo0873

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Dihydroorotase		PRO_0000325569
Sites
	Metal binding	13	1	Zinc 1 By similarity
	Metal binding	15	1	Zinc 1 By similarity
	Metal binding	99	1	Zinc 1; via carbamate group By similarity
	Metal binding	99	1	Zinc 2; via carbamate group By similarity
	Metal binding	136	1	Zinc 2 By similarity
	Metal binding	174	1	Zinc 2 By similarity
	Metal binding	247	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	99	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1K3T5-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: D7B70D3E29E5EDFA	FASTA	344	37,002
	10 20 30 40 50 60 MQSITFTRPD DWHLHVRDGA ALAAVVPHTA ERFGRALIMP NLRPPVTTTA QALAYRDRIR 70 80 90 100 110 120 AAVPAGLAFE PLMSLYLTDN TAPDEIERAR ASGAVIAAKL YPAGATTNSD AGVTAIDKIY 130 140 150 160 170 180 PVLERMEACG LVLCVHGEVT GGEVDVFDRE RVFMEKILSP LVRRFPALKV VFEHITTAEA 190 200 210 220 230 240 AQFVRAAGAN VAATVTAHHL LLNRNAIFAG GIRPHHYCLP VLKRETHRVA LVEAVTSGNP 250 260 270 280 290 300 RFFLGTDSAP HARSTKEAAC GCAGCYTAHA GIELYAEVFD AAGALERLEA FASLNGPAFY 310 320 330 340 GLAPNADKIT LVRESWSVPA GFPYLDDDPL VPLRAGESVG WRLA

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM406670 Genomic DNA. Translation: CAL93490.1.
RefSeq	YP_932377.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4606619.
GenomeReviews	Gene locus azo0873 in contig AM406670_GR.
KEGG	azo:azo0873.
Organism-specific databases
CMR	Search...
Family and domain databases
HAMAP	MF_00219. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. pyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
BLOCKS	Search...
ProtoNet	Search...

Entry information

Entry name

PYRC_AZOSB

Accession

Primary (citable) accession number: A1K3T5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	February 6, 2007
Last modified:	September 2, 2008
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)