A1K3B9 (AROE_AZOSB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Shikimate dehydrogenase
EC=1.1.1.25

Gene names

Name:	aroE
Ordered Locus Names:	azo0707

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP MF_00222
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro shikimate 3-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	Shikimate dehydrogenase HAMAP MF_00222		PRO_1000021256
Regions
	Nucleotide binding	130 – 134	5	NADP By similarity
Sites
	Active site	65	1	Proton acceptor Potential

Sequences

	Sequence		Length	Mass (Da)
	A1K3B9 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: EF62BBB00FAA584A	FASTA	281	28,497
	10 20 30 40 50 60 MDRYAVVGNP IAHSKSPQIH AAFAHQTGEA LGYEAILAPL DGFVDTVLAF RAVGGRGMNV 70 80 90 100 110 120 TVPFKLEAHA LATRLTPRAA AAGAVNTLAF GGPETPDDIL GDNTDGAGLV RDLTVNLGCP 130 140 150 160 170 180 LHGRRVLLLG AGGAARGALL PLLEQAPAAL TIANRTAAKA VELAAGFAAG HPGVAIDGGG 190 200 210 220 230 240 FDALAGRRFD VVINATAASL ADQAPPLPAG IYAEGALAYD MMYGRGDTPF LAAARADGAG 250 260 270 280 RLADGLGMLV EQAAESFLLW RGVRPDTAPV LADLRARLAA A

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: BH72.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM406670 Genomic DNA. Translation: CAL93324.1.
RefSeq	YP_932211.1. NC_008702.1.
3D structure databases
ProteinModelPortal	A1K3B9.
SMR	A1K3B9. Positions 1-279.
ModBase	Search...
Protein-protein interaction databases
STRING	A1K3B9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4609282.
GenomeReviews	Gene locus azo0707 in contig AM406670_GR.
KEGG	azo:azo0707.
PATRIC	21009690. VBIAzoSp26047_0720.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0169.
HOGENOM	HBG553408.
OMA	VVFGNPI.
PhylomeDB	A1K3B9.
ProtClustDB	PRK00258.
Enzyme and pathway databases
BioCyc	ASP62928:AZO0707-MONOMER.
Family and domain databases
HAMAP	MF_00222. Shikimate_DH_AroE. [Tree]
InterPro	IPR016040. NAD(P)-bd_dom. IPR011342. Shikimate_DH. IPR013708. Shikimate_DH-bd_N. IPR022893. Shikimate_quinate_DH. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00014.
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. AroE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROE_AZOSB

Accession

Primary (citable) accession number: A1K3B9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	December 14, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program