UniProtKB - A1K306 (DAPF_AZOSB)
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Protein
Diaminopimelate epimerase
Gene
dapF
Organism
Azoarcus sp. (strain BH72)
Status
Functioni
Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation
Catalytic activityi
LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation
: L-lysine biosynthesis via DAP pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate.UniRule annotationProteins known to be involved in this subpathway in this organism are:
- Diaminopimelate epimerase (dapF)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes DL-2,6-diaminopimelate from LL-2,6-diaminopimelate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 13 | SubstrateUniRule annotation | 1 | |
Binding sitei | 46 | SubstrateUniRule annotation | 1 | |
Binding sitei | 66 | SubstrateUniRule annotation | 1 | |
Active sitei | 75 | Proton donorUniRule annotation | 1 | |
Binding sitei | 159 | SubstrateUniRule annotation | 1 | |
Sitei | 161 | Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation | 1 | |
Binding sitei | 192 | SubstrateUniRule annotation | 1 | |
Sitei | 210 | Could be important to modulate the pK values of the two catalytic cysteine residuesUniRule annotation | 1 | |
Active sitei | 219 | Proton acceptorUniRule annotation | 1 |
GO - Molecular functioni
- diaminopimelate epimerase activity Source: UniProtKB-EC
GO - Biological processi
- lysine biosynthetic process via diaminopimelate Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Isomerase |
Biological process | Amino-acid biosynthesis, Lysine biosynthesis |
Enzyme and pathway databases
BioCyci | ASP62928:G1GJH-595-MONOMER. |
UniPathwayi | UPA00034; UER00025. |
Names & Taxonomyi
Protein namesi | Recommended name: Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)Short name: DAP epimeraseUniRule annotation Alternative name(s): PLP-independent amino acid racemaseUniRule annotation |
Gene namesi | Name:dapFUniRule annotation Ordered Locus Names:azo0594 |
Organismi | Azoarcus sp. (strain BH72) |
Taxonomic identifieri | 62928 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Zoogloeaceae › Azoarcus |
Proteomesi |
|
Subcellular locationi
- Cytoplasm UniRule annotation
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_1000011833 | 1 – 277 | Diaminopimelate epimeraseAdd BLAST | 277 |
Interactioni
Subunit structurei
Homodimer.UniRule annotation
Protein-protein interaction databases
STRINGi | 62928.azo0594. |
Structurei
3D structure databases
ProteinModelPortali | A1K306. |
SMRi | A1K306. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 76 – 77 | Substrate bindingUniRule annotation | 2 | |
Regioni | 210 – 211 | Substrate bindingUniRule annotation | 2 | |
Regioni | 220 – 221 | Substrate bindingUniRule annotation | 2 |
Sequence similaritiesi
Belongs to the diaminopimelate epimerase family.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG4105E4Z. Bacteria. COG0253. LUCA. |
HOGENOMi | HOG000220467. |
KOi | K01778. |
OMAi | SMCGNGG. |
OrthoDBi | POG091H01QC. |
Family and domain databases
HAMAPi | MF_00197. DAP_epimerase. 1 hit. |
InterProi | View protein in InterPro IPR018510. DAP_epimerase_AS. IPR001653. DAP_epimerase_DapF. |
PANTHERi | PTHR31689. PTHR31689. 1 hit. |
Pfami | View protein in Pfam PF01678. DAP_epimerase. 2 hits. |
TIGRFAMsi | TIGR00652. DapF. 1 hit. |
PROSITEi | View protein in PROSITE PS01326. DAP_EPIMERASE. 1 hit. |
i Sequence
Sequence statusi: Complete.
A1K306-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MHIRFTKMHG LGNDFVVIDA TRTPVDLTPA RVKAIADRHF GVGCDQLLVV
60 70 80 90 100
EPPGSDEVDF RYRIFNADGG EVEQCGNGAR CFVRFVHDKG LTDKREIRVE
110 120 130 140 150
TRAGVIAPAL RPDGLVSVDM GVPELSPARI PFVSDSDAWV QPLQLAEDTV
160 170 180 190 200
AITAVSMGNP HAVQVVADVD TAPVARQGAE IECHPRFPAR VNAGFMQVVD
210 220 230 240 250
AGHIRLRVFE RGAGETLACG TGACAAVVAG IGRGLLVSPV RVETRGGELE
260 270
IAWAGPGTPV VMTGPAVTVF EGELELP
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AM406670 Genomic DNA. Translation: CAL93211.1. |
RefSeqi | WP_011764329.1. NC_008702.1. |
Genome annotation databases
EnsemblBacteriai | CAL93211; CAL93211; azo0594. |
KEGGi | azo:azo0594. |
Similar proteinsi
Entry informationi
Entry namei | DAPF_AZOSB | |
Accessioni | A1K306Primary (citable) accession number: A1K306 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | February 6, 2007 | |
Last modified: | March 28, 2018 | |
This is version 69 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |