ID   A1K2K6_AZOSB            Unreviewed;       547 AA.
AC   A1K2K6;
DT   06-FEB-2007, integrated into UniProtKB/TrEMBL.
DT   06-FEB-2007, sequence version 1.
DT   27-MAR-2024, entry version 85.
DE   RecName: Full=DNA ligase (ATP) {ECO:0000256|ARBA:ARBA00012727};
DE            EC=6.5.1.1 {ECO:0000256|ARBA:ARBA00012727};
GN   Name=lig2 {ECO:0000313|EMBL:CAL93061.1};
GN   OrderedLocusNames=azo0444 {ECO:0000313|EMBL:CAL93061.1};
OS   Azoarcus sp. (strain BH72).
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Rhodocyclales; Zoogloeaceae;
OC   Azoarcus.
OX   NCBI_TaxID=418699 {ECO:0000313|EMBL:CAL93061.1, ECO:0000313|Proteomes:UP000002588};
RN   [1] {ECO:0000313|EMBL:CAL93061.1, ECO:0000313|Proteomes:UP000002588}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BH72 {ECO:0000313|EMBL:CAL93061.1,
RC   ECO:0000313|Proteomes:UP000002588};
RX   PubMed=17057704; DOI=10.1038/nbt1243;
RA   Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J.,
RA   Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C.,
RA   Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J.,
RA   Weidner S., Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A.;
RT   "Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp.
RT   strain BH72.";
RL   Nat. Biotechnol. 24:1385-1391(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC         (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP +
CC         diphosphate.; EC=6.5.1.1; Evidence={ECO:0000256|ARBA:ARBA00034003};
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DR   EMBL; AM406670; CAL93061.1; -; Genomic_DNA.
DR   RefSeq; WP_011764179.1; NC_008702.1.
DR   AlphaFoldDB; A1K2K6; -.
DR   STRING; 62928.azo0444; -.
DR   KEGG; azo:azo0444; -.
DR   eggNOG; COG1793; Bacteria.
DR   HOGENOM; CLU_005138_6_2_4; -.
DR   Proteomes; UP000002588; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:UniProtKB-EC.
DR   GO; GO:0006310; P:DNA recombination; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   CDD; cd07897; Adenylation_DNA_ligase_Bac1; 1.
DR   CDD; cd07972; OBF_DNA_ligase_Arch_LigB; 1.
DR   Gene3D; 1.10.3260.10; DNA ligase, ATP-dependent, N-terminal domain; 1.
DR   Gene3D; 3.30.470.30; DNA ligase/mRNA capping enzyme; 1.
DR   Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR   InterPro; IPR026333; ATP_dep_DNA_lig_pp_1105_fam.
DR   InterPro; IPR012309; DNA_ligase_ATP-dep_C.
DR   InterPro; IPR012310; DNA_ligase_ATP-dep_cent.
DR   InterPro; IPR016059; DNA_ligase_ATP-dep_CS.
DR   InterPro; IPR012308; DNA_ligase_ATP-dep_N.
DR   InterPro; IPR036599; DNA_ligase_N_sf.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   NCBIfam; TIGR04120; DNA_lig_bact; 1.
DR   PANTHER; PTHR45674; DNA LIGASE 1/3 FAMILY MEMBER; 1.
DR   PANTHER; PTHR45674:SF13; DNA LIGASE-RELATED; 1.
DR   Pfam; PF04679; DNA_ligase_A_C; 1.
DR   Pfam; PF01068; DNA_ligase_A_M; 1.
DR   Pfam; PF04675; DNA_ligase_A_N; 1.
DR   SUPFAM; SSF56091; DNA ligase/mRNA capping enzyme, catalytic domain; 1.
DR   SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR   PROSITE; PS00697; DNA_LIGASE_A1; 1.
DR   PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE   4: Predicted;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW   DNA replication {ECO:0000256|ARBA:ARBA00022705};
KW   Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:CAL93061.1};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002588}.
FT   DOMAIN          319..447
FT                   /note="ATP-dependent DNA ligase family profile"
FT                   /evidence="ECO:0000259|PROSITE:PS50160"
SQ   SEQUENCE   547 AA;  60601 MW;  5BC3D20EEE765BA1 CRC64;
     MKAFAALYAE LDATTSSNAK LAAMVAYFRA APAADAAWAA YFLAGGKPRQ LVPVRQLREF
     AMRIAGVPEW LFEESYQAVG DLAETLALLL PEGVAGDDAP LAEWVEARLL PLRGLPPDVL
     QPRLEMLFGA LSTLQRFVCA KLITGSFRVG VSRLLVTRAL GEAGGVDTRR VAERLIGYTD
     IGARPDAASY RALLAPPAPA EGAREDGLPY PFFLAHPLQA PVEALEDRLG PPENWLIEWK
     WDGIRAQLVR RGGRSWLWSR GEDLVTERFP ELAALGAELP EGTVIDGEIL VWKAGRVQPF
     AALQKRLGRK TVGTRLLAEA PVVLCAYDLL EWQGEDLRAQ PQHARRAALE RLAQHHPQRA
     LQLSPLLHGE DWAALARLRE EARERGVEGM MLKGRDAPYG VGRSKEGGVW WKWKIDPYSI
     DAVLIYAQRG HGRRASLYTD YTFAVWDAPP DAPQRRLVPF AKAYSGLTDA EIRQVDAIVR
     KTTQEKFGPV RSVTPEQVFE LGFEGIARSA RHKSGIAVRF PRILRWRHDK PAAEADTLQT
     LAELLPP
//