Reviewed, UniProtKB/Swiss-Prot A1K2E2 (SYR_AZOSB)

Names and origin

Protein names

Recommended name:
    Arginyl-tRNA synthetase
    EC=6.1.1.19
Alternative name(s):
    Arginine--tRNA ligase
      Short name=ArgRS

Gene names

Name:	argS
Ordered Locus Names:	azo0380

Organism

Azoarcus sp. (strain BH72) [Complete proteome] [HAMAP]

Taxonomic identifier

62928 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Rhodocyclales › Rhodocyclaceae › Azoarcus

Protein attributes

Sequence length	587 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-tRNA(Arg). HAMAP MF_00123
Subunit structure	Monomer By similarity. HAMAP MF_00123
Subcellular location	Cytoplasm HAMAP MF_00123.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP arginine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 587	587	Arginyl-tRNA synthetase HAMAP MF_00123		PRO_1000017986
Regions
	Motif	126 – 136	11	"HIGH" region HAMAP MF_00123

Sequences

	Sequence		Length	Mass (Da)
	A1K2E2-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 3363BA7A8BF9C2A0	FASTA	587	64,580
	10 20 30 40 50 60 MSADPKVLLT DLIKTALKSV APEHADTAIL LERPKQASHG DFATNVALQL AKPLKRNPRE 70 80 90 100 110 120 LAALLLAELP ASNLVAKTEV AGAGFINFTL AAAAKTAVVG EVLAKGADFG RGAKKNVKVQ 130 140 150 160 170 180 VEFVSANPTG PLHVGHGRGA AYGASLSDVL CFAGYDVTRE YYVNDAGRQM DILALSTWLR 190 200 210 220 230 240 YLALFGIDVP FPPNAYQGDY VIDMARGLRD AHQGRYAGVT LAQVLEGTPG LPVAERKDDE 250 260 270 280 290 300 AKQQRELHLD GLIANAKRLL GEDYPFVHGF ALNEQLGDGR DDLQEFGVHF DKWFSEKSLF 310 320 330 340 350 360 DTGLVERAVA ELEKRGHIYV QDGAKWFRST DFGDEKDRVV QRENGLYTYF ASDIAYHLNK 370 380 390 400 410 420 YERGFDRIID IWGADHHGYI PRVKGAIAAL GLPPEKLEVA LVQFAVLYRD GQKTSMSTRS 430 440 450 460 470 480 GEFVTLRELR REVGNDACRF FYVLRKSDQH LDFDLDLAKS QSNENPVYYI QYAHARVCSV 490 500 510 520 530 540 LNQWGGEPAE LQAADLGKLE NERELALCAR LAGFPEVVQG AAADYAPHQI AFYLKDLAAD 550 560 570 580 FHSWYNAERM LVDDEAVKLA RLALAAAVRS VLRGGLAVLG VSAPESM

References

[1]

"Complete genome of the mutualistic, N2-fixing grass endophyte Azoarcus sp. strain BH72." Krause A., Ramakumar A., Bartels D., Battistoni F., Bekel T., Boch J., Boehm M., Friedrich F., Hurek T., Krause L., Linke B., McHardy A.C., Sarkar A., Schneiker S., Syed A.A., Thauer R., Vorhoelter F.-J., Weidner S. , Puehler A., Reinhold-Hurek B., Kaiser O., Goesmann A. Nat. Biotechnol. 24:1385-1391(2006) [PubMed: 17057704] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM406670 Genomic DNA. Translation: CAL92997.1.
RefSeq	YP_931884.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A1K2E2.
Genome annotation databases
GeneID	4606369.
GenomeReviews	Gene locus azo0380 in contig AM406670_GR.
KEGG	azo:azo0380.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0018.
HOGENOM	HBG695395.
OMA	FYLDNAY.
Family and domain databases
HAMAP	MF_00123. Arg_tRNA_synth. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR001278. Arg-tRNA-synth_Ic. IPR015945. Arg-tRNA-synth_Ic_core. IPR005148. Arg-tRNA-synth_Ic_N. IPR008909. DALR_anticod_bd. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.30.1360.70. Arg-tRNA-synth_Ic_N. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11956. Arg_tRNA-synt_1c. 1 hit.
Pfam	PF03485. Arg_tRNA_synt_N. 1 hit. PF05746. DALR_1. 1 hit. PF00750. tRNA-synt_1d. 2 hits. [Graphical view]
PRINTS	PR01038. TRNASYNTHARG.
SMART	SM00836. DALR_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR00456. argS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYR_AZOSB

Accession

Primary (citable) accession number: A1K2E2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	February 9, 2010
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)