ID   MAO1_YERE8              Reviewed;         565 AA.
AC   A1JTY5;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   16-JUN-2009, entry version 23.
DE   RecName: Full=NAD-dependent malic enzyme;
DE            Short=NAD-ME;
DE            EC=1.1.1.38;
GN   Name=sfcA; OrderedLocusNames=YE2794;
OS   Yersinia enterocolitica serotype O:8 / biotype 1B (strain 8081).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Yersinia.
OX   NCBI_TaxID=393305;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17173484; DOI=10.1371/journal.pgen.0020206;
RA   Thomson N.R., Howard S., Wren B.W., Holden M.T.G., Crossman L.,
RA   Challis G.L., Churcher C., Mungall K., Brooks K., Chillingworth T.,
RA   Feltwell T., Abdellah Z., Hauser H., Jagels K., Maddison M., Moule S.,
RA   Sanders M., Whitehead S., Quail M.A., Dougan G., Parkhill J.,
RA   Prentice M.B.;
RT   "The complete genome sequence and comparative genome analysis of the
RT   high pathogenicity Yersinia enterocolitica strain 8081.";
RL   PLoS Genet. 2:2039-2051(2006).
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
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DR   EMBL; AM286415; CAL12827.1; -; Genomic_DNA.
DR   RefSeq; YP_001006984.1; -.
DR   GeneID; 4712450; -.
DR   GenomeReviews; AM286415_GR; YE2794.
DR   KEGG; yen:YE2794; -.
DR   NMPDR; fig|630.2.peg.2722; -.
DR   OMA; A1JTY5; IQDNNET.
DR   GO; GO:0016619; F:malate dehydrogenase (oxaloacetate-decarbox...; IEA:HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01619; -; 1.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Metal-binding; NAD; Oxidoreductase.
FT   CHAIN         1    565       NAD-dependent malic enzyme.
FT                                /FTId=PRO_1000069552.
FT   ACT_SITE    104    104       Proton donor (By similarity).
FT   ACT_SITE    175    175       Proton acceptor (By similarity).
FT   METAL       246    246       Divalent metal cation (By similarity).
FT   METAL       247    247       Divalent metal cation (By similarity).
FT   METAL       270    270       Divalent metal cation (By similarity).
FT   BINDING     157    157       NAD (By similarity).
FT   BINDING     270    270       NAD (By similarity).
FT   BINDING     418    418       NAD (By similarity).
FT   SITE        270    270       Important for activity (By similarity).
SQ   SEQUENCE   565 AA;  62672 MW;  433B11E44E441B40 CRC64;
     MELEYESKRP LYIPYAGPIL LEFPLLNKGS AFTNDERSHF NLHGLLPEAV ETIEEQAERA
     YRQYQDFKND DDKHIYLRNI QDTNETLFYR LLEAHLSEMM PIIYTPTVGE ACEHFSDIYR
     RARGLFISYP NREHIDDMLQ NATKQNVKVI VVTDGERILG LGDQGIGGMG IPIGKLSLYT
     ACGGISPAYT LPVVLDVGTN NPQRLNDPLY MGWRHPRISG DEYYAFVDEF IQAVKRRWPN
     VLLQFEDFAQ NNATPLLNRY RDELCCFNDD IQGTAAVTLG SLIAASHAAG SQLRDQTVTF
     LGAGSAGCGI AEQIIAQMIS EGLSEEQARA RVFMVDRFGL LTDKLPNLLD FQSKLVQKSD
     ALQSWNLTSD SISLQDVVRN AKPTVLIGVS GQPGLFTEEL IREMHKHCAR PIVMPLSNPT
     SRVEARPEDI INWTDGAALV ATGSPFSPVS YKEKLYPIAQ CNNSYIFPGI GLGVLASGAK
     RVTDGMLMAA SRALAQCSPL AQNGEGALLP NIDDIQAVSK TIAMQVGKAA QLQGVAIVTS
     EEALAKAIEH NYWQPQYRTY KRTSF
//