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A1JTC4 (BGAL_YERE8) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-galactosidase

Short name=Beta-gal
EC=3.2.1.23
Alternative name(s):
Lactase
Gene names
Name:lacZ
Ordered Locus Names:YE2592
OrganismYersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081) [Complete proteome] [HAMAP]
Taxonomic identifier393305 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia

Protein attributes

Sequence length1050 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. HAMAP-Rule MF_01687

Cofactor

Binds 2 magnesium ions per monomer By similarity. HAMAP-Rule MF_01687

Binds 1 sodium ion per monomer By similarity. HAMAP-Rule MF_01687

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_01687

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10501050Beta-galactosidase HAMAP-Rule MF_01687
PRO_0000367014

Regions

Region553 – 5564Substrate binding By similarity

Sites

Active site4771Proton donor By similarity
Active site5531Nucleophile By similarity
Metal binding2091Sodium By similarity
Metal binding4321Magnesium 1 By similarity
Metal binding4341Magnesium 1 By similarity
Metal binding4771Magnesium 1 By similarity
Metal binding6131Magnesium 2 By similarity
Metal binding6171Sodium; via carbonyl oxygen By similarity
Metal binding6201Sodium By similarity
Binding site1101Substrate By similarity
Binding site2091Substrate By similarity
Binding site4771Substrate By similarity
Binding site6201Substrate By similarity
Binding site10231Substrate By similarity
Site3731Transition state stabilizer By similarity
Site4071Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
A1JTC4 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: 86889BDB17379594

FASTA1,050121,234
        10         20         30         40         50         60 
MTAQQEVKPQ ATPALSQILF RRDWENPQIT QYNRLEAHPP FYSWRHLDAA QNDTPSPQRQ 

        70         80         90        100        110        120 
LLNGQWSFSY FTQPESVPDE WVEHDLPEAI SMPVPSNWQL HGYDIPIYTN VQYPIPVDPP 

       130        140        150        160        170        180 
RVPQNNPTGC YSYNFTLEPD WILSGQTRII FDGVNSAFYL WCNGRWVGYS QDSRLPAEFD 

       190        200        210        220        230        240 
LTPYLKAGNN RIAVLVLRWS DGSYLEDQDM WRMSGIFRDV SLLHKPDIHL RDIHISTHLS 

       250        260        270        280        290        300 
PEFSSAHLEV MAAVNIPLLD INNSQVTKAY QIQVQLWLAD SLVASLRQPL GTQPIDERGH 

       310        320        330        340        350        360 
YTDRTHLSLR VEHPLLWSAE QPALYRTVVS LLDSQQKLIE AEAYDVGFRQ VAIHQGLLKI 

       370        380        390        400        410        420 
NGKAVLIRGV NRHEHHPQTG QAIDEESMLQ DIILMKQHNF NAVRCSHYPN HPLWYRLCDR 

       430        440        450        460        470        480 
YGLYVVDEAN IETHGMQPMR RLADDPQWFS AFSERVTRMV QRDRNHPCII IWSLGNESGH 

       490        500        510        520        530        540 
GATHDALYRW IKTNDPTRPV QYEGGGANTQ ATDIVCPMYA RVDEDQPFPA VPKWAIKKWI 

       550        560        570        580        590        600 
GLPNESRPLI LCEYAHAMGN SFGGFARYWQ AFRQYPRLQG GFVWDWVDQS LTRNDENGQP 

       610        620        630        640        650        660 
YWAYGGDFGD SPNDRQFCMN GLVFPDRTPH PCLYEAQCAQ QFFQFSLVST SPLIIKVTSE 

       670        680        690        700        710        720 
YLFRNSDNEH LYWRIELAGK SVLEGSFPLD LLPESTQQFS LTERLPAICG PGDLWLNVEV 

       730        740        750        760        770        780 
RQVEETPWSP SHHRCAWFQW RLPHSLAVLS RGLSDSATSN NLKLHQDIQH ITVTHQQQHW 

       790        800        810        820        830        840 
QFNRQTGLLE QWCVGGENRL LTPLRDQFVR APLDNDIGIS ETTRIDPNAW VERWKKAGIY 

       850        860        870        880        890        900 
QLEQRCLSLH ADTLSQAIQI SAEYIYEFAQ EQLLHTHWLY RFDQQGHMTI DVRVQIATSL 

       910        920        930        940        950        960 
PSLARVGMCC QLSDIYENVE WLGLGPHENY PDRQLSAQHS HWSQPLDQMH TPYIFPSENG 

       970        980        990       1000       1010       1020 
LRCNTSMLSY GNWQLTGQFH FGISRYSTQQ LMAASHQHLL RSEAGTWLNI DGFHMGVGGD 

      1030       1040       1050 
DSWSPSVHAD NLLTNEIYQY QVCWQYKDSI 

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References

[1]"The complete genome sequence and comparative genome analysis of the high pathogenicity Yersinia enterocolitica strain 8081."
Thomson N.R., Howard S., Wren B.W., Holden M.T.G., Crossman L., Challis G.L., Churcher C., Mungall K., Brooks K., Chillingworth T., Feltwell T., Abdellah Z., Hauser H., Jagels K., Maddison M., Moule S., Sanders M., Whitehead S. expand/collapse author list , Quail M.A., Dougan G., Parkhill J., Prentice M.B.
PLoS Genet. 2:2039-2051(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCTC 13174 / 8081.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM286415 Genomic DNA. Translation: CAL12630.1.
RefSeqYP_001006792.1. NC_008800.1.

3D structure databases

ProteinModelPortalA1JTC4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING393305.YE2592.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL12630; CAL12630; YE2592.
GeneID4714238.
KEGGyen:YE2592.
PATRIC18564786. VBIYerEnt11519_2750.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHOG000252443.
KOK01190.
OMADPKKPYI.
OrthoDBEOG6XWV0T.

Family and domain databases

Gene3D2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_01687. Beta_gal.
InterProIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSPR00132. GLHYDRLASE2.
SMARTSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameBGAL_YERE8
AccessionPrimary (citable) accession number: A1JTC4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: February 6, 2007
Last modified: May 14, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries