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A1JTC4

- BGAL_YERE8

UniProt

A1JTC4 - BGAL_YERE8

Protein

Beta-galactosidase

Gene

lacZ

Organism
Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (06 Feb 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

    Cofactori

    Binds 2 magnesium ions per monomer.UniRule annotation
    Binds 1 sodium ion per monomer.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei110 – 1101SubstrateUniRule annotation
    Metal bindingi209 – 2091SodiumUniRule annotation
    Binding sitei209 – 2091SubstrateUniRule annotation
    Sitei373 – 3731Transition state stabilizerUniRule annotation
    Sitei407 – 4071Transition state stabilizerUniRule annotation
    Metal bindingi432 – 4321Magnesium 1UniRule annotation
    Metal bindingi434 – 4341Magnesium 1UniRule annotation
    Active sitei477 – 4771Proton donorUniRule annotation
    Metal bindingi477 – 4771Magnesium 1UniRule annotation
    Binding sitei477 – 4771SubstrateUniRule annotation
    Active sitei553 – 5531NucleophileUniRule annotation
    Metal bindingi613 – 6131Magnesium 2UniRule annotation
    Metal bindingi617 – 6171Sodium; via carbonyl oxygenUniRule annotation
    Metal bindingi620 – 6201SodiumUniRule annotation
    Binding sitei620 – 6201SubstrateUniRule annotation
    Binding sitei1023 – 10231SubstrateUniRule annotation

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB-EC
    2. carbohydrate binding Source: InterPro
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Sodium

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
    Short name:
    Beta-galUniRule annotation
    Alternative name(s):
    LactaseUniRule annotation
    Gene namesi
    Name:lacZUniRule annotation
    Ordered Locus Names:YE2592
    OrganismiYersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
    Taxonomic identifieri393305 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
    ProteomesiUP000000642: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. beta-galactosidase complex Source: InterPro

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10501050Beta-galactosidasePRO_0000367014Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Protein-protein interaction databases

    STRINGi393305.YE2592.

    Structurei

    3D structure databases

    ProteinModelPortaliA1JTC4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni553 – 5564Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000252443.
    KOiK01190.
    OMAiDPKKPYI.
    OrthoDBiEOG6XWV0T.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_01687. Beta_gal.
    InterProiIPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    PRINTSiPR00132. GLHYDRLASE2.
    SMARTiSM01038. Bgal_small_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A1JTC4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTAQQEVKPQ ATPALSQILF RRDWENPQIT QYNRLEAHPP FYSWRHLDAA     50
    QNDTPSPQRQ LLNGQWSFSY FTQPESVPDE WVEHDLPEAI SMPVPSNWQL 100
    HGYDIPIYTN VQYPIPVDPP RVPQNNPTGC YSYNFTLEPD WILSGQTRII 150
    FDGVNSAFYL WCNGRWVGYS QDSRLPAEFD LTPYLKAGNN RIAVLVLRWS 200
    DGSYLEDQDM WRMSGIFRDV SLLHKPDIHL RDIHISTHLS PEFSSAHLEV 250
    MAAVNIPLLD INNSQVTKAY QIQVQLWLAD SLVASLRQPL GTQPIDERGH 300
    YTDRTHLSLR VEHPLLWSAE QPALYRTVVS LLDSQQKLIE AEAYDVGFRQ 350
    VAIHQGLLKI NGKAVLIRGV NRHEHHPQTG QAIDEESMLQ DIILMKQHNF 400
    NAVRCSHYPN HPLWYRLCDR YGLYVVDEAN IETHGMQPMR RLADDPQWFS 450
    AFSERVTRMV QRDRNHPCII IWSLGNESGH GATHDALYRW IKTNDPTRPV 500
    QYEGGGANTQ ATDIVCPMYA RVDEDQPFPA VPKWAIKKWI GLPNESRPLI 550
    LCEYAHAMGN SFGGFARYWQ AFRQYPRLQG GFVWDWVDQS LTRNDENGQP 600
    YWAYGGDFGD SPNDRQFCMN GLVFPDRTPH PCLYEAQCAQ QFFQFSLVST 650
    SPLIIKVTSE YLFRNSDNEH LYWRIELAGK SVLEGSFPLD LLPESTQQFS 700
    LTERLPAICG PGDLWLNVEV RQVEETPWSP SHHRCAWFQW RLPHSLAVLS 750
    RGLSDSATSN NLKLHQDIQH ITVTHQQQHW QFNRQTGLLE QWCVGGENRL 800
    LTPLRDQFVR APLDNDIGIS ETTRIDPNAW VERWKKAGIY QLEQRCLSLH 850
    ADTLSQAIQI SAEYIYEFAQ EQLLHTHWLY RFDQQGHMTI DVRVQIATSL 900
    PSLARVGMCC QLSDIYENVE WLGLGPHENY PDRQLSAQHS HWSQPLDQMH 950
    TPYIFPSENG LRCNTSMLSY GNWQLTGQFH FGISRYSTQQ LMAASHQHLL 1000
    RSEAGTWLNI DGFHMGVGGD DSWSPSVHAD NLLTNEIYQY QVCWQYKDSI 1050
    Length:1,050
    Mass (Da):121,234
    Last modified:February 6, 2007 - v1
    Checksum:i86889BDB17379594
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM286415 Genomic DNA. Translation: CAL12630.1.
    RefSeqiYP_001006792.1. NC_008800.1.

    Genome annotation databases

    EnsemblBacteriaiCAL12630; CAL12630; YE2592.
    GeneIDi4714238.
    KEGGiyen:YE2592.
    PATRICi18564786. VBIYerEnt11519_2750.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AM286415 Genomic DNA. Translation: CAL12630.1 .
    RefSeqi YP_001006792.1. NC_008800.1.

    3D structure databases

    ProteinModelPortali A1JTC4.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 393305.YE2592.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAL12630 ; CAL12630 ; YE2592 .
    GeneIDi 4714238.
    KEGGi yen:YE2592.
    PATRICi 18564786. VBIYerEnt11519_2750.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000252443.
    KOi K01190.
    OMAi DPKKPYI.
    OrthoDBi EOG6XWV0T.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    2.60.40.320. 2 hits.
    2.70.98.10. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_01687. Beta_gal.
    InterProi IPR004199. B-gal_small/dom_5.
    IPR011013. Gal_mutarotase_SF_dom.
    IPR008979. Galactose-bd-like.
    IPR014718. Glyco_hydro-type_carb-bd_sub.
    IPR006101. Glyco_hydro_2.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR023232. Glyco_hydro_2_AS.
    IPR023933. Glyco_hydro_2_beta_Galsidase.
    IPR023230. Glyco_hydro_2_CS.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02929. Bgal_small_N. 1 hit.
    PF00703. Glyco_hydro_2. 1 hit.
    PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00132. GLHYDRLASE2.
    SMARTi SM01038. Bgal_small_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 2 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    SSF74650. SSF74650. 1 hit.
    PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
    PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NCTC 13174 / 8081.

    Entry informationi

    Entry nameiBGAL_YERE8
    AccessioniPrimary (citable) accession number: A1JTC4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: February 6, 2007
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3