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A1JTC4

- BGAL_YERE8

UniProt

A1JTC4 - BGAL_YERE8

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Protein

Beta-galactosidase

Gene

lacZ

Organism
Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotationNote: Binds 2 magnesium ions per monomer.UniRule annotation
  • Na(+)UniRule annotationNote: Binds 1 sodium ion per monomer.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei110 – 1101SubstrateUniRule annotation
Metal bindingi209 – 2091SodiumUniRule annotation
Binding sitei209 – 2091SubstrateUniRule annotation
Sitei373 – 3731Transition state stabilizerUniRule annotation
Sitei407 – 4071Transition state stabilizerUniRule annotation
Metal bindingi432 – 4321Magnesium 1UniRule annotation
Metal bindingi434 – 4341Magnesium 1UniRule annotation
Active sitei477 – 4771Proton donorUniRule annotation
Metal bindingi477 – 4771Magnesium 1UniRule annotation
Binding sitei477 – 4771SubstrateUniRule annotation
Active sitei553 – 5531NucleophileUniRule annotation
Metal bindingi613 – 6131Magnesium 2UniRule annotation
Metal bindingi617 – 6171Sodium; via carbonyl oxygenUniRule annotation
Metal bindingi620 – 6201SodiumUniRule annotation
Binding sitei620 – 6201SubstrateUniRule annotation
Binding sitei1023 – 10231SubstrateUniRule annotation

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB-EC
  2. carbohydrate binding Source: InterPro
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Sodium

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidaseUniRule annotation (EC:3.2.1.23UniRule annotation)
Short name:
Beta-galUniRule annotation
Alternative name(s):
LactaseUniRule annotation
Gene namesi
Name:lacZUniRule annotation
Ordered Locus Names:YE2592
OrganismiYersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Taxonomic identifieri393305 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000000642: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. beta-galactosidase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10501050Beta-galactosidasePRO_0000367014Add
BLAST

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi393305.YE2592.

Structurei

3D structure databases

ProteinModelPortaliA1JTC4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni553 – 5564Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000252443.
KOiK01190.
OMAiDPKKPYI.
OrthoDBiEOG6XWV0T.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_01687. Beta_gal.
InterProiIPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
PRINTSiPR00132. GLHYDRLASE2.
SMARTiSM01038. Bgal_small_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEiPS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1JTC4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTAQQEVKPQ ATPALSQILF RRDWENPQIT QYNRLEAHPP FYSWRHLDAA
60 70 80 90 100
QNDTPSPQRQ LLNGQWSFSY FTQPESVPDE WVEHDLPEAI SMPVPSNWQL
110 120 130 140 150
HGYDIPIYTN VQYPIPVDPP RVPQNNPTGC YSYNFTLEPD WILSGQTRII
160 170 180 190 200
FDGVNSAFYL WCNGRWVGYS QDSRLPAEFD LTPYLKAGNN RIAVLVLRWS
210 220 230 240 250
DGSYLEDQDM WRMSGIFRDV SLLHKPDIHL RDIHISTHLS PEFSSAHLEV
260 270 280 290 300
MAAVNIPLLD INNSQVTKAY QIQVQLWLAD SLVASLRQPL GTQPIDERGH
310 320 330 340 350
YTDRTHLSLR VEHPLLWSAE QPALYRTVVS LLDSQQKLIE AEAYDVGFRQ
360 370 380 390 400
VAIHQGLLKI NGKAVLIRGV NRHEHHPQTG QAIDEESMLQ DIILMKQHNF
410 420 430 440 450
NAVRCSHYPN HPLWYRLCDR YGLYVVDEAN IETHGMQPMR RLADDPQWFS
460 470 480 490 500
AFSERVTRMV QRDRNHPCII IWSLGNESGH GATHDALYRW IKTNDPTRPV
510 520 530 540 550
QYEGGGANTQ ATDIVCPMYA RVDEDQPFPA VPKWAIKKWI GLPNESRPLI
560 570 580 590 600
LCEYAHAMGN SFGGFARYWQ AFRQYPRLQG GFVWDWVDQS LTRNDENGQP
610 620 630 640 650
YWAYGGDFGD SPNDRQFCMN GLVFPDRTPH PCLYEAQCAQ QFFQFSLVST
660 670 680 690 700
SPLIIKVTSE YLFRNSDNEH LYWRIELAGK SVLEGSFPLD LLPESTQQFS
710 720 730 740 750
LTERLPAICG PGDLWLNVEV RQVEETPWSP SHHRCAWFQW RLPHSLAVLS
760 770 780 790 800
RGLSDSATSN NLKLHQDIQH ITVTHQQQHW QFNRQTGLLE QWCVGGENRL
810 820 830 840 850
LTPLRDQFVR APLDNDIGIS ETTRIDPNAW VERWKKAGIY QLEQRCLSLH
860 870 880 890 900
ADTLSQAIQI SAEYIYEFAQ EQLLHTHWLY RFDQQGHMTI DVRVQIATSL
910 920 930 940 950
PSLARVGMCC QLSDIYENVE WLGLGPHENY PDRQLSAQHS HWSQPLDQMH
960 970 980 990 1000
TPYIFPSENG LRCNTSMLSY GNWQLTGQFH FGISRYSTQQ LMAASHQHLL
1010 1020 1030 1040 1050
RSEAGTWLNI DGFHMGVGGD DSWSPSVHAD NLLTNEIYQY QVCWQYKDSI
Length:1,050
Mass (Da):121,234
Last modified:February 6, 2007 - v1
Checksum:i86889BDB17379594
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286415 Genomic DNA. Translation: CAL12630.1.
RefSeqiWP_011816626.1. NC_008800.1.
YP_001006792.1. NC_008800.1.

Genome annotation databases

EnsemblBacteriaiCAL12630; CAL12630; YE2592.
GeneIDi4714238.
KEGGiyen:YE2592.
PATRICi18564786. VBIYerEnt11519_2750.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286415 Genomic DNA. Translation: CAL12630.1 .
RefSeqi WP_011816626.1. NC_008800.1.
YP_001006792.1. NC_008800.1.

3D structure databases

ProteinModelPortali A1JTC4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 393305.YE2592.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL12630 ; CAL12630 ; YE2592 .
GeneIDi 4714238.
KEGGi yen:YE2592.
PATRICi 18564786. VBIYerEnt11519_2750.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000252443.
KOi K01190.
OMAi DPKKPYI.
OrthoDBi EOG6XWV0T.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
2.60.40.320. 2 hits.
2.70.98.10. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_01687. Beta_gal.
InterProi IPR004199. B-gal_small/dom_5.
IPR011013. Gal_mutarotase_SF_dom.
IPR008979. Galactose-bd-like.
IPR014718. Glyco_hydro-type_carb-bd_sub.
IPR006101. Glyco_hydro_2.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR023232. Glyco_hydro_2_AS.
IPR023933. Glyco_hydro_2_beta_Galsidase.
IPR023230. Glyco_hydro_2_CS.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02929. Bgal_small_N. 1 hit.
PF00703. Glyco_hydro_2. 1 hit.
PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
PRINTSi PR00132. GLHYDRLASE2.
SMARTi SM01038. Bgal_small_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
SSF74650. SSF74650. 1 hit.
PROSITEi PS00719. GLYCOSYL_HYDROL_F2_1. 1 hit.
PS00608. GLYCOSYL_HYDROL_F2_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 13174 / 8081.

Entry informationi

Entry nameiBGAL_YERE8
AccessioniPrimary (citable) accession number: A1JTC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: February 6, 2007
Last modified: November 26, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3