Reviewed, UniProtKB/Swiss-Prot A1JT62 (GHRB_YERE8)

Names and origin

Protein names

Recommended name:
    Glyoxylate/hydroxypyruvate reductase B
    EC=1.1.1.79
    EC=1.1.1.81

Gene names

Name:	ghrB
Ordered Locus Names:	YE4159

Organism

Yersinia enterocolitica serotype O:8 / biotype 1B (strain 8081) [Complete proteome] [HAMAP]

Taxonomic identifier

393305 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity. HAMAP MF_01667
Catalytic activity	Glycolate + NADP+ = glyoxylate + NADPH. HAMAP MF_01667 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP MF_01667
Subunit structure	Homodimer By similarity. HAMAP MF_01667
Subcellular location	Cytoplasm Probable HAMAP MF_01667.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activity Inferred from electronic annotation. Source: HAMAP hydroxypyruvate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Glyoxylate/hydroxypyruvate reductase B HAMAP MF_01667		PRO_0000348405
Sites
	Active site	237	1	By similarity
	Active site	266	1	By similarity
	Active site	285	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1JT62-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 44D7E34E4330849E	FASTA	326	35,256
	10 20 30 40 50 60 MKPSIVLYKS IPADLHQRLE QHFTVNSFEG LSSDNQPELL SALQQAEGLI GSGGKIDQAF 70 80 90 100 110 120 LERAPKLRAA STISVGYDNF DVDALSQRGI ALMHTPTVLT ETVADTMMAL VLSSARRVVE 130 140 150 160 170 180 LAERVKAGEW QDSIGDDWFG VDVHHKTIGI LGMGRIGMAL AQRAHFGFSM PVLYTSRRPH 190 200 210 220 230 240 EAAEKRFGAR RCSLDTLLAE VDFLCITLPM TEQTYHMIGP EQLAKMKSSA ILINAGRGPV 250 260 270 280 290 300 VDEQALIAAL QDGTIHAAGL DVFAQEPLPV ESPLLKLPNV VAVPHIGSAT HETRYNMAAC 310 320 AVDNLIAALT GTVTENCVNP QVLQQA

References

[1]

"The complete genome sequence and comparative genome analysis of the high pathogenicity Yersinia enterocolitica strain 8081." Thomson N.R., Howard S., Wren B.W., Holden M.T.G., Crossman L., Challis G.L., Churcher C., Mungall K., Brooks K., Chillingworth T., Feltwell T., Abdellah Z., Hauser H., Jagels K., Maddison M., Moule S., Sanders M., Whitehead S. , Quail M.A., Dougan G., Parkhill J., Prentice M.B. PLoS Genet. 2:2039-2051(2006) [PubMed: 17173484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM286415 Genomic DNA. Translation: CAL14175.1.
RefSeq	YP_001008293.1.
3D structure databases
SMR	A1JT62. Positions 1-324.
ModBase	Search...
Protein-protein interaction databases
STRING	A1JT62.
Genome annotation databases
GeneID	4716553.
GenomeReviews	Gene locus YE4159 in contig AM286415_GR.
KEGG	yen:YE4159.
NMPDR	fig|630.2.peg.4084.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1052.
HOGENOM	HBG731446.
OMA	DSPLRTH.
PhylomeDB	A1JT62.
Family and domain databases
HAMAP	MF_01667. 2-Hacid_dh_C_GhrB. [Tree]
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. 1 hit. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GHRB_YERE8

Accession

Primary (citable) accession number: A1JT62

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	February 6, 2007
Last modified:	January 19, 2010
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)