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A1JSJ0 (GLGB_YERE8) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:YE4013
OrganismYersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081) [Complete proteome] [HAMAP]
Taxonomic identifier393305 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia

Protein attributes

Sequence length727 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7277271,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_1000045006

Sites

Active site4051Nucleophile By similarity
Active site4581Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
A1JSJ0 [UniParc].

Last modified February 6, 2007. Version 1.
Checksum: C7B3DDA329DEAF7E

FASTA72783,951
        10         20         30         40         50         60 
MSVLPDRQVI NQIISGHYAD PFSVLGMHQT ERGLQVCALL PDAQEVWLID TKTGRRVAQL 

        70         80         90        100        110        120 
DCEDPRGFFA VQLTRRKNPF RYQFAVTWQE STQIIEDPYR FGTLLQDIDT WLLAEGTHLR 

       130        140        150        160        170        180 
PYERLGAHLM SLDEVPGVSF AVWAPNAQRV SVVGEFNFWD GRRHPMRLRR ENGIWELFLP 

       190        200        210        220        230        240 
GVQAGQLYKF EIIDCHGQVR LKADPYAFKA QMRPETASLI SPLPDVVENT PARQKANDLR 

       250        260        270        280        290        300 
SPISIYEVHL GSWRRHTDNN FWLSYGELAD QLVEYVKYMG FTHVELLPIN EHPFDGSWGY 

       310        320        330        340        350        360 
QPLGLYAPTR RFGTPQDFKD FVAKFHEAGI NVILDWVPGH FPSDEHGLST FDGTALYEYA 

       370        380        390        400        410        420 
DPREGYHQDW NTLIYNYGRN EVRNYLAGNA FYWMERFGID ALRIDAVASM IYRDYSRAEG 

       430        440        450        460        470        480 
QWVPNYYGGR ENLEAIAFLR YTNHTIGVER PGGVTMAEES TDFPGVTLPP DAGGLGFNYK 

       490        500        510        520        530        540 
WNMGWMHDTL NYMQCDPVHR KYHHNLMTFG MLYAYTENFI LPISHDEVVH GKRSVLDRMP 

       550        560        570        580        590        600 
GDAWQKFANL RAYYGFMWAH PGKKLLFMGC EFAQGREWNF DTSLDWHLLD DENGWHKGVQ 

       610        620        630        640        650        660 
RWVRDLNHCY QQYAPLYELD YQPAGFEWLV VDDHENSVFA FLRRDADGNE LIVISNFTPV 

       670        680        690        700        710        720 
PRYNYRVGVP QSGHYREVLN SDSAFYRGSN MGNQGGIHSH PVSSHNHAHS LLLTLPPLST 


IYLTRGG 

« Hide

References

[1]"The complete genome sequence and comparative genome analysis of the high pathogenicity Yersinia enterocolitica strain 8081."
Thomson N.R., Howard S., Wren B.W., Holden M.T.G., Crossman L., Challis G.L., Churcher C., Mungall K., Brooks K., Chillingworth T., Feltwell T., Abdellah Z., Hauser H., Jagels K., Maddison M., Moule S., Sanders M., Whitehead S. expand/collapse author list , Quail M.A., Dougan G., Parkhill J., Prentice M.B.
PLoS Genet. 2:2039-2051(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCTC 13174 / 8081.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AM286415 Genomic DNA. Translation: CAL14031.1.
RefSeqYP_001008157.1. NC_008800.1.

3D structure databases

ProteinModelPortalA1JSJ0.
SMRA1JSJ0. Positions 117-725.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING393305.YE4013.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL14031; CAL14031; YE4013.
GeneID4716273.
KEGGyen:YE4013.
PATRIC18567894. VBIYerEnt11519_4272.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAGELSHAW.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_YERE8
AccessionPrimary (citable) accession number: A1JSJ0
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: June 11, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries