Reviewed, UniProtKB/Swiss-Prot A1JS35 (ASTB_YERE8)

Names and origin

Protein names

Recommended name:
    N-succinylarginine dihydrolase
    EC=3.5.3.23

Gene names

Name:	astB
Ordered Locus Names:	YE2466

Organism

Yersinia enterocolitica serotype O:8 / biotype 1B (strain 8081) [Complete proteome] [HAMAP]

Taxonomic identifier

393305 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydrolysis of N(2)-succinylarginine into N(2)-succinylornithine, ammonia and CO2 By similarity.
Catalytic activity	N(2)-succinyl-L-arginine + 2 H2O = N(2)-succinyl-L-ornithine + 2 NH3 + CO2. HAMAP MF_01172
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 2/5. HAMAP MF_01172
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the succinylarginine dihydrolase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP
Molecular function	N-succinylarginine dihydrolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	N-succinylarginine dihydrolase HAMAP MF_01172		PRO_1000065742
Regions
	Region	19 – 28	10	Substrate binding By similarity
	Region	137 – 138	2	Substrate binding By similarity
Sites
	Active site	174	1	By similarity
	Active site	249	1	By similarity
	Active site	370	1	Nucleophile By similarity
	Binding site	110	1	Substrate By similarity
	Binding site	213	1	Substrate By similarity
	Binding site	251	1	Substrate By similarity
	Binding site	364	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1JS35-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 99C6026B739549C0	FASTA	447	49,368
	10 20 30 40 50 60 MAGYEVNFDG LVGLTHHYAG LSFGNEASTR HQNTLSNPRL AAKQGLLKMK ALADLGYKQG 70 80 90 100 110 120 VLPPQERPAM GVLRQLGFSG SDEQVLSEVV RKSPRLLSAV SSASSMWTAN AATVSPAADS 130 140 150 160 170 180 ADGRVHFTVA NLNNKFHRAI EADTTSAILK SIFNNHRHFV HHDALPSVEL FGDEGAANHN 190 200 210 220 230 240 RLGGEYDRPA IQVFVYGRQG FEGGAMPSRY PARQTLEASE AVARLHLLDP ERAVFVQQNP 250 260 270 280 290 300 AVIDQGVFHN DVIAVSNQNV LFHHQHAFVP DIRVMEDLRR KMGRIEQQLF TIEVPAAQVS 310 320 330 340 350 360 VAQAVSSYLF NSQLLSKANG KMLLVIPQES QECPAVWEYL SELINSGGPI DEVRVFDLRE 370 380 390 400 410 420 SMHNGGGPAC LRLRVALNDT ELAAVNSRVM MTPALFVALN NWVDQHYRDR LQFKDLADPQ 430 440 LLQEGRQALD ELTKILNLGS IYPFQHL

References

[1]

"The complete genome sequence and comparative genome analysis of the high pathogenicity Yersinia enterocolitica strain 8081." Thomson N.R., Howard S., Wren B.W., Holden M.T.G., Crossman L., Challis G.L., Churcher C., Mungall K., Brooks K., Chillingworth T., Feltwell T., Abdellah Z., Hauser H., Jagels K., Maddison M., Moule S., Sanders M., Whitehead S. , Quail M.A., Dougan G., Parkhill J., Prentice M.B. PLoS Genet. 2:2039-2051(2006) [PubMed: 17173484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM286415 Genomic DNA. Translation: CAL12509.1.
RefSeq	YP_001006673.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4712504.
GenomeReviews	Gene locus YE2466 in contig AM286415_GR.
KEGG	yen:YE2466.
NMPDR	fig|630.2.peg.2389.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A1JS35. HFAHHPA.
Family and domain databases
HAMAP	MF_01172. [Tree]
InterPro	IPR007079. SuccinylArg_d-Hdrlase_AstB. [Graphical view]
Gene3D	G3DSA:3.75.10.20. SuccinylArg_di_hydro. 1 hit.
Pfam	PF04996. AstB. 1 hit. [Graphical view]
TIGRFAMs	TIGR03241. arg_catab_astB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTB_YERE8

Accession

Primary (citable) accession number: A1JS35

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	February 6, 2007
Last modified:	June 16, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)