Reviewed, UniProtKB/Swiss-Prot A1JS32 (ASTE_YERE8)

Names and origin

Protein names

Recommended name:
    Succinylglutamate desuccinylase
    EC=3.5.1.96

Gene names

Name:	astE
Ordered Locus Names:	YE2465

Organism

Yersinia enterocolitica serotype O:8 / biotype 1B (strain 8081) [Complete proteome] [HAMAP]

Taxonomic identifier

393305 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	330 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Transforms N(2)-succinylglutamate into succinate and glutamate By similarity.
Catalytic activity	N-succinyl-L-glutamate + H2O = succinate + L-glutamate. HAMAP MF_00767
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 5/5. HAMAP MF_00767
Sequence similarities	Belongs to the aspA/astE family. Succinylglutamate desuccinylase subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: HAMAP succinylglutamate desuccinylase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 330	330	Succinylglutamate desuccinylase HAMAP MF_00767		PRO_1000017334
Sites
	Active site	210	1	Potential
	Metal binding	53	1	Zinc By similarity
	Metal binding	56	1	Zinc By similarity
	Metal binding	147	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1JS32-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: B86527782F6E931B	FASTA	330	36,775
	10 20 30 40 50 60 MPDFLSITLS GVPPSLTSGE TSNLKWQWVD EGVLMLTPHG ACSQSVVLSA GIHGNETAPI 70 80 90 100 110 120 EILNQLVSDL LASQLPLAVR LLVILGNPPA IRAGERYLTA DINRMFGGRY KNHSLTDEAR 130 140 150 160 170 180 RAEILEQKVG EFFASDPLSL RLHYDLHTAI RGSHHNRFGL LPYRTTPYCA AMLRWLKDSE 190 200 210 220 230 240 LDALVMHTSA GGTFAHFSSE FCQAASCTLE LGKALPFGQN QLEQFRPITA GLRALVSGGQ 250 260 270 280 290 300 LPTRSTEAMI FYRVVKSLLK QHADFKLWVA DDTVNFTRYP QGTLMIEQLN EHYCVEHEYE 310 320 330 WILFPNPRVA LGLRAGIMLV QMDENDLLQA

References

[1]

"The complete genome sequence and comparative genome analysis of the high pathogenicity Yersinia enterocolitica strain 8081." Thomson N.R., Howard S., Wren B.W., Holden M.T.G., Crossman L., Challis G.L., Churcher C., Mungall K., Brooks K., Chillingworth T., Feltwell T., Abdellah Z., Hauser H., Jagels K., Maddison M., Moule S., Sanders M., Whitehead S. , Quail M.A., Dougan G., Parkhill J., Prentice M.B. PLoS Genet. 2:2039-2051(2006) [PubMed: 17173484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM286415 Genomic DNA. Translation: CAL12508.1.
RefSeq	YP_001006672.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4712503.
GenomeReviews	Gene locus YE2465 in contig AM286415_GR.
KEGG	yen:YE2465.
NMPDR	fig|630.2.peg.2388.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A1JS32. EKFAIYP.
Family and domain databases
HAMAP	MF_00767. [Tree]
InterPro	IPR007036. Aste_AspA. IPR016681. SuccinylGlu_desuccinylase. [Graphical view]
Pfam	PF04952. AstE_AspA. 1 hit. [Graphical view]
PIRSF	PIRSF017020. AstE. 1 hit.
TIGRFAMs	TIGR03242. arg_catab_astE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTE_YERE8

Accession

Primary (citable) accession number: A1JS32

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	February 6, 2007
Last modified:	June 16, 2009
This is version 20 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)