Reviewed, UniProtKB/Swiss-Prot A1JRR3 (GHRA_YERE8)

Names and origin

Protein names

Recommended name:
    Glyoxylate/hydroxypyruvate reductase A
    EC=1.1.1.79
    EC=1.1.1.81
Alternative name(s):
    2-ketoacid reductase

Gene names

Name:	ghrA
Ordered Locus Names:	YE2422

Organism

Yersinia enterocolitica serotype O:8 / biotype 1B (strain 8081) [Complete proteome] [HAMAP]

Taxonomic identifier

393305 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	313 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity.
Catalytic activity	Glycolate + NADP+ = glyoxylate + NADPH. HAMAP MF_01666 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP MF_01666
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	cofactor binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activity Inferred from electronic annotation. Source: HAMAP hydroxypyruvate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 313	313	Glyoxylate/hydroxypyruvate reductase A HAMAP MF_01666		PRO_0000348378
Sites
	Active site	228	1	By similarity
	Active site	276	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A1JRR3-1 [UniParc]. Last modified February 6, 2007. Version 1. Checksum: 29D79951FB1E61DD	FASTA	313	34,854
	10 20 30 40 50 60 MNIIFYHPFF EAKQWLSGLQ SRLPTANIRQ WRRGDTQPAD YALVWQPPQE MLASRVELKG 70 80 90 100 110 120 VFALGAGVDA ILDQERRHPG TLPAGVPLVR LEDTGMSLQM QEYVVATVLR YFRRMDEYQL 130 140 150 160 170 180 QQQQKLWQPL EPHQHDKFTI GILGAGVLGK SVAHKLAEFG FTVRCWSRTP KQIDGVTSFA 190 200 210 220 230 240 GQEKLPAFIQ GTQLLINLLP HTPQTAGILN QSLFSQLNAN AYIINIARGA HLLERDLLAA 250 260 270 280 290 300 MNAGQVAAAT LDVFAEEPLP SMHPFWSHPR VTITPHIAAV TLPEVAMDQV VANIQAMEAG 310 REPVGLVDVV RGY

References

[1]

"The complete genome sequence and comparative genome analysis of the high pathogenicity Yersinia enterocolitica strain 8081." Thomson N.R., Howard S., Wren B.W., Holden M.T.G., Crossman L., Challis G.L., Churcher C., Mungall K., Brooks K., Chillingworth T., Feltwell T., Abdellah Z., Hauser H., Jagels K., Maddison M., Moule S., Sanders M., Whitehead S. , Quail M.A., Dougan G., Parkhill J., Prentice M.B. PLoS Genet. 2:2039-2051(2006) [PubMed: 17173484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM286415 Genomic DNA. Translation: CAL12470.1.
RefSeq	YP_001006636.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4714839.
GenomeReviews	Gene locus YE2422 in contig AM286415_GR.
KEGG	yen:YE2422.
NMPDR	fig|630.2.peg.2347.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A1JRR3. YALVWRA.
Family and domain databases
HAMAP	MF_01666. [Tree]
InterPro	IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GHRA_YERE8

Accession

Primary (citable) accession number: A1JRR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	February 6, 2007
Last modified:	June 16, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)