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Protein

DNA topoisomerase 3

Gene

topB

Organism
Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationNote: Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi8Magnesium 1; catalyticUniRule annotation1
Metal bindingi104Magnesium 1; catalyticUniRule annotation1
Metal bindingi104Magnesium 2UniRule annotation1
Metal bindingi106Magnesium 2UniRule annotation1
Active sitei329O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase, TopoisomeraseUniRule annotationSAAS annotation

Keywords - Ligandi

DNA-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IIIUniRule annotation
Gene namesi
Name:topBUniRule annotationImported
Ordered Locus Names:YE2257Imported
OrganismiYersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)Imported
Taxonomic identifieri393305 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesYersiniaceaeYersinia
Proteomesi
  • UP000000642 Componenti: Chromosome

Interactioni

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei62Interaction with DNAUniRule annotation1
Sitei171Interaction with DNAUniRule annotation1
Sitei179Interaction with DNAUniRule annotation1
Sitei186Interaction with DNAUniRule annotation1
Sitei331Interaction with DNAUniRule annotation1

Protein-protein interaction databases

STRINGi393305.YE2257.

Structurei

3D structure databases

ProteinModelPortaliA1JQE7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 135ToprimInterPro annotationAdd BLAST134

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni195 – 200Interaction with DNAUniRule annotation6

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotationSAAS annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108IYH. Bacteria.
COG0550. LUCA.
HOGENOMiHOG000086848.
KOiK03169.
OMAiFCPDAEY.
OrthoDBiPOG091H028X.

Family and domain databases

CDDicd00186. TOP1Ac. 1 hit.
Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00953. Topoisom_3_prok. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 2 hits.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01056. topB. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1JQE7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMRLFIAEKP SLARAIADIL PKPHRRGDGF IACGSDNMVT WCVGHLLEQA
60 70 80 90 100
QPDAYDSRYA RWSLADLPII PEKWRLQPRP SVSKQLNVIK QLLQQADEVI
110 120 130 140 150
HAGDPDREGQ LLVDEVLDYL DLAAEKRQNV RRCLINDLNP QAVERAVERL
160 170 180 190 200
RYNREFIPLC VSALARARAD WLYGINMTRA YTILGRNAGY DGVLSVGRVQ
210 220 230 240 250
TPVLGLVVRR DEEIEDFVAK DFFEVKAHIV TPADERFIAL WQPSDSCESY
260 270 280 290 300
QDEEGRLLHR PLAEHVVNRI AGQPALVTSY NDKRESETAP LPFSLSTLQI
310 320 330 340 350
EAAKRFGLSA QQVLDICQRL YETHKLITYP RSDCRYLPEE HFAGRHSVLN
360 370 380 390 400
AISMHQADLL PLDVMDSDRR NRCWDDKKVD AHHAIIPTAR ASKVNLTTDE
410 420 430 440 450
SKVYRLVAQQ YLMQFCPDAQ FRKCVIELDI AGGKFIAKAR FLAEAGWRTL
460 470 480 490 500
LGSKERDEEN EGAPLPVVEK GAELLCERGE VVERQTQPPR PFTDASLLSA
510 520 530 540 550
MTGIARFVQD KELKKVLRAT DGLGTEATRA GIIELLFKRT FLFKKGRYIH
560 570 580 590 600
ASPAGRALIH ALPDIAARPD MTAHWESTLT QISEKNCRYQ DFMQPLVATL
610 620 630 640
QDLISQAKQN RSPQVFRGLP AAPSGAIKKR KKTSSKTKEN KS
Length:642
Mass (Da):72,560
Last modified:February 6, 2007 - v1
Checksum:i016B4ED0A767C827
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286415 Genomic DNA. Translation: CAL12317.1.
RefSeqiWP_011816425.1. NC_008800.1.
YP_001006485.1. NC_008800.1.

Genome annotation databases

EnsemblBacteriaiCAL12317; CAL12317; YE2257.
GeneIDi4715370.
KEGGiyen:YE2257.
PATRICi18564116. VBIYerEnt11519_2417.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286415 Genomic DNA. Translation: CAL12317.1.
RefSeqiWP_011816425.1. NC_008800.1.
YP_001006485.1. NC_008800.1.

3D structure databases

ProteinModelPortaliA1JQE7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi393305.YE2257.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL12317; CAL12317; YE2257.
GeneIDi4715370.
KEGGiyen:YE2257.
PATRICi18564116. VBIYerEnt11519_2417.

Phylogenomic databases

eggNOGiENOG4108IYH. Bacteria.
COG0550. LUCA.
HOGENOMiHOG000086848.
KOiK03169.
OMAiFCPDAEY.
OrthoDBiPOG091H028X.

Family and domain databases

CDDicd00186. TOP1Ac. 1 hit.
Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00953. Topoisom_3_prok. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 2 hits.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01056. topB. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiA1JQE7_YERE8
AccessioniPrimary (citable) accession number: A1JQE7
Entry historyi
Integrated into UniProtKB/TrEMBL: February 6, 2007
Last sequence update: February 6, 2007
Last modified: November 2, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.