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Protein

Bifunctional protein Aas

Gene

aas

Organism
Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Plays a role in lysophospholipid acylation. Transfers fatty acids to the 1-position via an enzyme-bound acyl-ACP intermediate in the presence of ATP and magnesium. Its physiological function is to regenerate phosphatidylethanolamine from 2-acyl-glycero-3-phosphoethanolamine (2-acyl-GPE) formed by transacylation reactions or degradation by phospholipase A1.UniRule annotation

Catalytic activityi

Acyl-[acyl-carrier-protein] + O-(2-acyl-sn-glycero-3-phospho)ethanolamine = [acyl-carrier-protein] + O-(1-beta-acyl-2-acyl-sn-glycero-3-phospho)ethanolamine.UniRule annotation
ATP + a long-chain fatty acid + an [acyl-carrier-protein] = AMP + diphosphate + a long-chain acyl-[acyl-carrier-protein].UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei36 – 361UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Ligase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein AasUniRule annotation
Including the following 2 domains:
2-acylglycerophosphoethanolamine acyltransferaseUniRule annotation (EC:2.3.1.40UniRule annotation)
Alternative name(s):
2-acyl-GPE acyltransferaseUniRule annotation
Acyl-[acyl-carrier-protein]--phospholipid O-acyltransferaseUniRule annotation
Acyl-[acyl-carrier-protein] synthetaseUniRule annotation (EC:6.2.1.20UniRule annotation)
Alternative name(s):
Acyl-ACP synthetaseUniRule annotation
Long-chain-fatty-acid--[acyl-carrier-protein] ligaseUniRule annotation
Gene namesi
Name:aasUniRule annotation
Ordered Locus Names:YE3327
OrganismiYersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Taxonomic identifieri393305 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
Proteomesi
  • UP000000642 Componenti: Chromosome

Subcellular locationi

  • Cell inner membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei258 – 27720HelicalUniRule annotationAdd
BLAST
Transmembranei409 – 43325HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 718718Bifunctional protein AasPRO_1000065645Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi393305.YE3327.

Structurei

3D structure databases

ProteinModelPortaliA1JPF0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni15 – 138124AcyltransferaseAdd
BLAST
Regioni233 – 646414AMP-bindingAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the 2-acyl-GPE acetyltransferase family.UniRule annotation
In the C-terminal section; belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CEY. Bacteria.
COG0204. LUCA.
COG0318. LUCA.
HOGENOMiHOG000004907.
KOiK05939.
OMAiANWVYLE.
OrthoDBiPOG091H0GSG.

Family and domain databases

HAMAPiMF_01162. Aas. 1 hit.
InterProiIPR023775. Aas.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1JPF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYRLLRALF RGLFRVTIDG ITDQFSHQKL IITPNHVSFL DGALLALFLP
60 70 80 90 100
IKPVFAVYSN ITESWYMRWL KPYVDFVALD PTKPMAIKHL VRMVEQGRPV
110 120 130 140 150
VIFPEGRITV TGSLMKIYDG AAFVAAKSGA AVVPIRLEGP EFTRFGRLGD
160 170 180 190 200
VLKVRWFPKI SIHVLPATTL PMPQAPRARD RRVLAGERLH AIMMAARMAI
210 220 230 240 250
VPRETLFEAL LSAQTRYGRF KPCIEDISFK EDSYQTLLKK ILGVSRILQR
260 270 280 290 300
FTAQGEHVGM LLPNATITAA AIFGATLRGR IPALLNYTSG AKGLKSAITA
310 320 330 340 350
ASLKTIITSR QFLEKGKLTH LPEQVTEANW VYLEDLKDTV TLADKLWILF
360 370 380 390 400
HLCCPRRAMV PQQADDSALI LFTSGSEGNP KGVVHSHASL LANVEQIRTI
410 420 430 440 450
ADFTPRDRFM SSLPLFHAFG LTVGLFTPLM TGSRVFLYPS PLHYRVVPEL
460 470 480 490 500
VYDRNCTVLF GTSTFLGNYA RFAHPYDFAR LRYVVAGAEK LADSTKQIWQ
510 520 530 540 550
DKFGIRILEG YGVTECAPVV AINVPMAAKV NTVGRILPGM ESRVIPVPGI
560 570 580 590 600
EQGGRLQLRG PNIMRGYLRV EKPGVLEQPS AENTQGEQEA GWYDTGDIVA
610 620 630 640 650
IDEQGFCTIR GRMKRFAKLA GEMVSLESVE QLVLRISPEG QHAAATKTDS
660 670 680 690 700
AKGEALVLFT TDSEITREKL VKAARESGVP ELAVPRDIRV VKALPLLGSG
710
KPDFVTLSKM AEDPEMSA
Length:718
Mass (Da):79,473
Last modified:February 6, 2007 - v1
Checksum:i2F8F2774A5950426
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286415 Genomic DNA. Translation: CAL13356.1.
RefSeqiWP_011816988.1. NC_008800.1.
YP_001007500.1. NC_008800.1.

Genome annotation databases

EnsemblBacteriaiCAL13356; CAL13356; YE3327.
GeneIDi4713136.
KEGGiyen:YE3327.
PATRICi18566401. VBIYerEnt11519_3535.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286415 Genomic DNA. Translation: CAL13356.1.
RefSeqiWP_011816988.1. NC_008800.1.
YP_001007500.1. NC_008800.1.

3D structure databases

ProteinModelPortaliA1JPF0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi393305.YE3327.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL13356; CAL13356; YE3327.
GeneIDi4713136.
KEGGiyen:YE3327.
PATRICi18566401. VBIYerEnt11519_3535.

Phylogenomic databases

eggNOGiENOG4105CEY. Bacteria.
COG0204. LUCA.
COG0318. LUCA.
HOGENOMiHOG000004907.
KOiK05939.
OMAiANWVYLE.
OrthoDBiPOG091H0GSG.

Family and domain databases

HAMAPiMF_01162. Aas. 1 hit.
InterProiIPR023775. Aas.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAAS_YERE8
AccessioniPrimary (citable) accession number: A1JPF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: February 6, 2007
Last modified: September 7, 2016
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.