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A1JP85

- GLND_YERE8

UniProt

A1JP85 - GLND_YERE8

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene

glnD

Organism
Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
Short name:
UTase/URUniRule annotation
Alternative name(s):
Bifunctional [protein-PII] modification enzymeUniRule annotation
Bifunctional nitrogen sensor proteinUniRule annotation
Including the following 2 domains:
[Protein-PII] uridylyltransferaseUniRule annotation (EC:2.7.7.59UniRule annotation)
Short name:
PII uridylyltransferaseUniRule annotation
Short name:
UTaseUniRule annotation
[Protein-PII]-UMP uridylyl-removing enzymeUniRule annotation (EC:3.1.4.-UniRule annotation)
Short name:
URUniRule annotation
Gene namesi
Name:glnDUniRule annotation
Ordered Locus Names:YE3286
OrganismiYersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Taxonomic identifieri393305 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000000642: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 892892Bifunctional uridylyltransferase/uridylyl-removing enzymePRO_1000022356Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi393305.YE3286.

Structurei

3D structure databases

ProteinModelPortaliA1JP85.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini470 – 603134HDUniRule annotationAdd
BLAST
Domaini711 – 79282ACT 1UniRule annotationAdd
BLAST
Domaini818 – 89275ACT 2UniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 351351UridylyltransferaseAdd
BLAST
Regioni352 – 710359Uridylyl-removingAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.UniRule annotation
Contains 2 ACT domains.UniRule annotation
Contains 1 HD domain.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1JP85-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSDNHTEHSV LPAVTKVIPE QPASPATYLD SELNCPELKQ RLETFQSWLA
60 70 80 90 100
DAFNGGISAE TLIAARSDYI DRLLGRLWTF YGFDDVPETA LVAVGGYGRG
110 120 130 140 150
ELHPLSDIDV LILSKQRLND EHAQRVGQLI TLLWDLKLEV GHSVRTLEEC
160 170 180 190 200
LLEGLADLTV ATNMVESRLI CGDVALFLQM QKHIFSDNFW PSPKFFHAKV
210 220 230 240 250
VEQQERHKRY HGTSYNLEPD IKSSPGGLRD IHTLQWVARR HFGATSLSEM
260 270 280 290 300
VDFGFLTKAE RNELIESQSF LWRIRFALHL VLTRYDNRLL FDRQLSVAQL
310 320 330 340 350
LQYQGEGNEP VERMMKDFYR MTRRVSELNN MLLQLFDEAI LALDTNEKPR
360 370 380 390 400
PLDDEFQLRG DLIDLRDENL FVDKPEAIMR MFYLMVRNQD IKGIYSTTVR
410 420 430 440 450
RLRHARRHLK QPLCNIPEAR KLFMAILRHP GAVSRALLPM HRHSVLWAYM
460 470 480 490 500
PQWGSIVGQM QFDLFHAYTV DEHTIRVLLK LESFADESTR PRHPLCVELY
510 520 530 540 550
PRLPQPELLL LAALFHDIAK GRGGDHSILG AHDVLEFAEQ HGLNSREAQL
560 570 580 590 600
VAWLVRCHLL MSVTAQRRDI QDPAVIQQFS AEVQSETRLR YLVSLTVADI
610 620 630 640 650
CATNENLWNS WKQSLLRELY FATEKQLRRG MQNSPDLRER VRHHRLQALA
660 670 680 690 700
LLRMDNIDEE ALHHIWSRCR ADYFLRHSPN QLAWHARHLL EHDSTKPLVL
710 720 730 740 750
VSRQATRGGT EIFIWCPDRP SLFAAVVGEL DRRNLSVHDA QIFTNRDGMA
760 770 780 790 800
MDTFIVLEPD GSPLAQDRHP IIIHALQQAM TRQNYQHPRV RRLSPKLRHF
810 820 830 840 850
SVPTETNFLP THNERRTYLE LIALDQPGLL ARVGDIFADL GLSLHSARIT
860 870 880 890
TIGERVEDLF VLADKDRRAL SIETRRELAQ RLTDTLNPND KL
Length:892
Mass (Da):103,044
Last modified:February 6, 2007 - v1
Checksum:iF9A01583B3D41D50
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286415 Genomic DNA. Translation: CAL13316.1.
RefSeqiYP_001007460.1. NC_008800.1.

Genome annotation databases

EnsemblBacteriaiCAL13316; CAL13316; YE3286.
GeneIDi4713381.
KEGGiyen:YE3286.
PATRICi18566317. VBIYerEnt11519_3496.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM286415 Genomic DNA. Translation: CAL13316.1 .
RefSeqi YP_001007460.1. NC_008800.1.

3D structure databases

ProteinModelPortali A1JP85.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 393305.YE3286.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL13316 ; CAL13316 ; YE3286 .
GeneIDi 4713381.
KEGGi yen:YE3286.
PATRICi 18566317. VBIYerEnt11519_3496.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 13174 / 8081.

Entry informationi

Entry nameiGLND_YERE8
AccessioniPrimary (citable) accession number: A1JP85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: November 26, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3