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A1JP85

- GLND_YERE8

UniProt

A1JP85 - GLND_YERE8

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Protein
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Gene
glnD, YE3286
Organism
Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:YE3286
OrganismiYersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081)
Taxonomic identifieri393305 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
ProteomesiUP000000642: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 892892Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotation
PRO_1000022356Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi393305.YE3286.

Structurei

3D structure databases

ProteinModelPortaliA1JP85.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini470 – 603134HD
Add
BLAST
Domaini711 – 79282ACT 1
Add
BLAST
Domaini818 – 89275ACT 2
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 351351UridylyltransferaseUniRule annotation
Add
BLAST
Regioni352 – 710359Uridylyl-removingUniRule annotation
Add
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A1JP85-1 [UniParc]FASTAAdd to Basket

« Hide

MSDNHTEHSV LPAVTKVIPE QPASPATYLD SELNCPELKQ RLETFQSWLA    50
DAFNGGISAE TLIAARSDYI DRLLGRLWTF YGFDDVPETA LVAVGGYGRG 100
ELHPLSDIDV LILSKQRLND EHAQRVGQLI TLLWDLKLEV GHSVRTLEEC 150
LLEGLADLTV ATNMVESRLI CGDVALFLQM QKHIFSDNFW PSPKFFHAKV 200
VEQQERHKRY HGTSYNLEPD IKSSPGGLRD IHTLQWVARR HFGATSLSEM 250
VDFGFLTKAE RNELIESQSF LWRIRFALHL VLTRYDNRLL FDRQLSVAQL 300
LQYQGEGNEP VERMMKDFYR MTRRVSELNN MLLQLFDEAI LALDTNEKPR 350
PLDDEFQLRG DLIDLRDENL FVDKPEAIMR MFYLMVRNQD IKGIYSTTVR 400
RLRHARRHLK QPLCNIPEAR KLFMAILRHP GAVSRALLPM HRHSVLWAYM 450
PQWGSIVGQM QFDLFHAYTV DEHTIRVLLK LESFADESTR PRHPLCVELY 500
PRLPQPELLL LAALFHDIAK GRGGDHSILG AHDVLEFAEQ HGLNSREAQL 550
VAWLVRCHLL MSVTAQRRDI QDPAVIQQFS AEVQSETRLR YLVSLTVADI 600
CATNENLWNS WKQSLLRELY FATEKQLRRG MQNSPDLRER VRHHRLQALA 650
LLRMDNIDEE ALHHIWSRCR ADYFLRHSPN QLAWHARHLL EHDSTKPLVL 700
VSRQATRGGT EIFIWCPDRP SLFAAVVGEL DRRNLSVHDA QIFTNRDGMA 750
MDTFIVLEPD GSPLAQDRHP IIIHALQQAM TRQNYQHPRV RRLSPKLRHF 800
SVPTETNFLP THNERRTYLE LIALDQPGLL ARVGDIFADL GLSLHSARIT 850
TIGERVEDLF VLADKDRRAL SIETRRELAQ RLTDTLNPND KL 892
Length:892
Mass (Da):103,044
Last modified:February 6, 2007 - v1
Checksum:iF9A01583B3D41D50
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM286415 Genomic DNA. Translation: CAL13316.1.
RefSeqiYP_001007460.1. NC_008800.1.

Genome annotation databases

EnsemblBacteriaiCAL13316; CAL13316; YE3286.
GeneIDi4713381.
KEGGiyen:YE3286.
PATRICi18566317. VBIYerEnt11519_3496.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AM286415 Genomic DNA. Translation: CAL13316.1 .
RefSeqi YP_001007460.1. NC_008800.1.

3D structure databases

ProteinModelPortali A1JP85.
ModBasei Search...

Protein-protein interaction databases

STRINGi 393305.YE3286.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL13316 ; CAL13316 ; YE3286 .
GeneIDi 4713381.
KEGGi yen:YE3286.
PATRICi 18566317. VBIYerEnt11519_3496.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 2 hits.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 13174 / 8081.

Entry informationi

Entry nameiGLND_YERE8
AccessioniPrimary (citable) accession number: A1JP85
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: February 6, 2007
Last modified: June 11, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi